Histamine-3 receptor antagonists

ABSTRACT

This invention is directed to a compound of formula I,  
                 
as defined herein, or a pharmaceutically acceptable salt thereof; a pharmaceutical composition containing a compound of formula I, a process of preparation of a compound of formula I, a method of treatment of a disorder or condition that may be treated by antagonizing histamine H3 receptors, the method comprising administering to a mammal in need of such treatment a compound of formula I as described above, and a method of treatment of a disorder or condition selected from the group consisting of depression, mood disorders, schizophrenia, anxiety disorders, Alzheimer&#39;s disease, attention-deficit hyperactivity disorder (ADHD), psychotic disorders, cognitive disorders, sleep disorders, obesity, dizziness, epilepsy, motion sickness, respiratory diseases, allergy, allergy-induced airway responses, allergic rhinitis, nasal congestion, allergic congestion, congestion, hypotension, cardiovascular disease, diseases of the GI tract, hyper and hypo motility and acidic secretion of the gastro-intestinal tract, the method comprising administering to a mammal in need of such treatment a compound of formula I as described above.

BACKGROUND OF THE INVENTION

This invention is directed to compounds of formula I described herein, to a pharmaceutical composition comprising such compounds, to a process of preparation of such compounds, and to methods of treatment of disorders or conditions that may be treated by antagonizing histamine-3 (H3) receptors using such compounds.

Histamine is a well-known mediator in hypersensitive reactions (e.g. allergies, hay fever, and asthma) that are commonly treated with antagonists of histamine or “antihistamines.” It has also been established that histamine receptors exist in at least two distinct types, referred to as H1 and H2 receptors.

A third histamine receptor (H3 receptor) is believed to play a role in neurotransmission in the central nervous system, where the H3 receptor is thought to be disposed presynaptically on histaminergic nerve endings (Nature, 302, S32-837 (1983). The existence of the H3 receptor has been confirmed by the development of selective H3 receptor agonists and antagonists (Nature, 327, 117-123 (1987)) and has subsequently been shown to regulate the release of the neurotransmitters in both the central nervous system and peripheral organs, particularly the lungs, cardiovascular system and gastrointestinal tract.

A number of diseases or conditions may be treated with histamine-3 receptor ligands wherein the H3 ligand may be an antagonist, agonist or partial agonist, see: (Imamura et al., Circ. Res., (1996) 78, 475-481); (Imamura et. al., Circ. Res., (1996) 78, 863-869); (Lin et al., Brain Res. (1990) 523, 325-330); (Monti et al., Neuropsychopharmacology (1996) 15, 31 35); (Sakai, et al., Life Sci. (1991) 48, 2397-2404); (Mazurkiewiez-Kwilecki and Nsonwah, Can. J. Physiol. Pharmacol. (1989) 67, 75-78); (Panula, P. et al., Neuroscience (1998) 44, 465-481); (Wada et al., Trends in Neuroscience (1991) 14,415); (Monti et al., Eur. J. Pharmacol. (1991) 205, 283); (Mazurkiewicz-Kwilecki and Nsonwah, Can. J. Physiol. Pharmacol. (1989) 67, 75-78); (Haas et al., Behav. Brain Res. (1995) 66, 41-44); (De Almeida and Izquierdo, Arch. Int. Pharmacodyn. (1986) 283, 193-198); (Kamei et al., Psychopharmacology (1990) 102, 312-318); (Kamei and Sakata, Japan. J. Pharmacol. (1991) 57, 437-482): (Schwartz et al., Psychopharmacology; The fourth Generation of Progress, Bloom and Kupfer (eds.), Raven Press, New York, (1995) 3 97); (Shaywitz et al. Psychopharmacology (1984) 82, 73-77); (Dumery and Blozovski, Exp. Brain Res. (1987) 67, 61-69); (Tedford et al., J. Pharmacol. Exp. Ther. (1995) 275, 598-604); (Tedford et al., Soc. Neurosci. Abstr. (1996) 22, 22); Yokoyama et al., Eur. J. Pharmacol. (1993) 234, 129); (Yokoyama and Iinuma, CNS Drugs (1996) 5, 321); (Onodera et al., Prog. Neurobiol. (1994) 42, 685); (Leurs and Timmerman, Prog. Drug Res. (1992) 39, 127); (The Histamine H3 Receptor, Leurs and Timmerman (ed.), Elsevier Science, Amsterdam, The Netherlands (1998); (Leurs et al., Trends in Pharm. Sci. (1998) 19, 177-183); (Phillips et al., Annual Reports in Medicinal Chemistry (1998) 33, 31-40); (Matsubara et al., Eur. J. Pharmacol. (1992) 224, 145); (Rouleau et al., J. Pharmacol. Exp. Ther. (1997) 281, 1085); (Adam Szelag, “Role of histamine H3-receptors in the proliferation of neoplastic cells in vitro”, Med. Sci. Monit., 4(5): 747-755, (1998)); (Fitzsimons, C., H. Duran, F. Labombarda, B. Molinari and E. Rivera, “Histamine receptors signalling in epidermal tumor cell lines with H-ras gene alterations”, Inflammation Res., 47 (Suppl. 1): S50-S51 (1998)); (R. Leurs, R. C. Vollinga and H. Timmerman, “The medicinal chemistry and therapeutic potentials of ligand of the histamine H3 receptor”, Progress in Drug Research 45: 170-165, (1995)); (R. Levi and N. C. E. Smith, “Histamine H3-receptors: A new frontier in myocardial ischemia”, J. Pharm. Exp. Ther., 292: 825-830, (2000)); (Hatta, E., K Yasuda and R. Levi, “Activation of histamine H3 receptors inhibits carrier-mediated norepinephrine release in a human model of protracted myocardial ischemia”, J. Pharm. Exp. Ther., 283: 494-500, (1997); (H. Yokoyama and K. Iinuma, “Histamine and Seizures: Implications for the treatment of epilepsy”, CNS Drugs, 5(5); 321-330, 1995)); (K. Hurukami, H. Yokoyama, K. Onodera, K. Iinuma and T. Watanabe, AQ-0 145, “A newly developed histamine H3 antagonist, decreased seizure susceptibility of electrically induced convulsions in mice”. Meth. Find. Exp. Clin. Pharmacol., 17(C): 70-73, (1995); (Delaunois A., Gustin P., Garbarg M., and Ansay M., “Modulation of acetylcholine, capsaicin and substance P effects by histamine H3 receptors in isolated perfused rabbit lungs”, European Journal of Pharmacology 277(2-3):243-50, (1995)); and (Dimitriadou, et al., “Functional relationship between mast cells and C-sensitive nerve fibres evidenced by histamine H3-receptor modulation in rat lung and spleen”, Clinical Science 87(2)151-63, (1994). Such diseases or conditions include cardiovascular disorders such as acute myocardial infarction; memory processes, dementia and cognitive disorders such as Alzheimer's disease and attention-deficit hyperactivity disorder; neurological disorders such as Parkinson's disease, schizophrenia, depression, epilepsy, and seizures or convulsions; cancer such as cutaneous carcinoma, medullary thyroid carcinoma and melanoma; respiratory disorders such as asthma; sleep disorders such as narcolepsy; vestibular dysfunction such as Meniere's disease; gastrointestinal disorders, inflammation, migraine, motion sickness, obesity, pain, and septic shock.

H3 receptor antagonists have also been previously described in, for example, WO 03/050099, WO 02/0769252, WO 02/12224, and U.S. Patent Pubtication No. 2005/0171181 A1. The histamine H3 receptor (H3R) regulates the release of histamine and other neurotransmitters, including serotonin and acetylcholine. H-3R is relatively neuron specific and inhibits the release of certain monoamines such as histamine. Selective antagonism of H3R receptors raises brain histamine levels and inhibits such activities as food consumption while minimizing non-specific peripheral consequences. Antagonists of the receptor increase synthesis and release of cerebral histamine and other monoamines. By this mechanism, they induce a prolonged wakefulness, improved cognitive function, reduction in food intake and normalization of vestibular reflexes. Accordingly, the receptor is an important target for new therapeutics in Alzheimer disease, mood disorders and cognitive disorders, including attention deficit hyperactive disorder (ADHD), attention deficit disorder (ADD), cognitive deficiencies, obesity, dizziness, schizophrenia, epilepsy, sleeping disorders, narcolepsy and motion sickness, and various forms of anxiety.

The majority of histamine H3 receptor antagonists to date resemble histamine in possessing an imidazole ring that may be substituted, as described, for example, in WO 96/38142. Non-imidazole neuroactive compounds such as beta histamines (Arrang, Eur. J. Pharm. 1985, 111:72-84) demonstrated some histamine H3 receptor activity but with poor potency. EP 978512 and EP 0982300A2 disclose non-imidazole alkyamines as histamine H3 receptor antagonists. WO 02/12224 (Ortho McNeil Pharmaceuticals) describes non-imidazole bicyclic derivatives as histamine H3 receptor ligands. Other receptor antagonists have been described in WO 02/32893 and WO 02/06233.

The compounds of this invention are highly selective for the H3 receptor (vs. other histamine receptors), and possess remarkable drug disposition properties (pharmacokinetics). In particular, the compounds of this invention selectively distinguish H3R from the other receptor subtypes H1R, H2R. In view of the increased level of interest in histamine H3 receptor agonists, inverse agonists and antagonists in the art, novel compounds that interact with the histamine H3 receptor would be a highly desirable contribution to the art. The present invention provides such a contribution to the art being based on the finding that a novel class of cyclobutyl reverse amides has a high and specific affinity to the histamine H3 receptor and have a superior drug profile.

SUMMARY OF THE INVENTION

This invention is directed to a compound of formula I:

or a pharmaceutically acceptable salt thereof, wherein:

R¹ and R² are each independently selected from the group consisting of hydrogen;

C₁-C₈ alkyl optionally substituted with 1 to 4 halogens;

C₁-C₄ alkyl group optionally substituted with a substituent selected from the group consisting of OH, one to four C₁-C₄ alkyl, C₃-C₇ cycloalkyl, C₁-C₄ dialkylamino, C₈-C₁₄ aryl optionally substituted with a halogen and optionally substituted with C₈-C₁₀ aryloxy optionally substituted with one to two halogens, and 5-10-membered heteroaryl optionally substituted with a C₈-C₁₀ aryl group and optionally substituted with one to three C₁-C₄ alkyl groups;

C₃-C₇ cycloalkyl;

C₈-C₁₄aryl;

—(C₀-C₃)alkyl-O—(C₁-C₃)alkyl optionally substituted with (C₁-C₃)alkyl;

—(C₁-C₃)alkyl-C(═O)O—(C₁-C₃)alkyl;

3-8-membered heterocycloalkyl optionally substituted with one or more C₁-C₄ alkyl-carbonyl groups;

C₈-C₁₀ arylsulfonyl optionally substituted with one or more C₁-C₂ alkyl;

5-10-membered heteroaryl; and

C₈-C₁₄ aryl-C₀-C₄ alkylene-O—C₀-C₄ alkyl, wherein each C₀-C₄ alkyl and each C₀-C₄ alkylene is optionally substituted with one to four C₁-C₄ alkyl;

or optionally R¹ and R², together with the nitrogen to which they are attached, form a 4-, 5-, 6-, or 7-membered saturated or unsaturated aliphatic ring, wherein one of the carbons in said aliphatic ring is optionally replaced by O, S, NR³, or CO, and wherein said ring is optionally fused to a C₈-C₁₀ arylene and is optionally substituted at a ring carbon with a substituent selected from the group consisting of

—OH, 5-10-membered heteroaryl optionally substituted with one or more halogens and optionally substituted with one or more C₁-C₂ alkyl,

C₁-C₄ alkoxy optionally substituted with one or more C₁-C₂ alkoxy and optionally substituted with one or more C₁-C₄ dialkylaminocarbonyl, and

one or two C₁-C₄ alkyl optionally and independently substituted with one or more C₁-C₂ alkoxy;

wherein R³ is

hydrogen;

C₁-C₈ alkyl optionally substituted with 1 to 4 halogens;

5-10-membered heteroaryl optionally substituted with a substituent selected from the group consisting of halogen, C₁-C₄ alkyl, C₁-C₂ alkoxy, C₈-C₁₀ aryl, C₁-C₄ alkylaminocarbonyl, and cyano;

C₁-C₄ alkyl group optionally substituted with a substituent selected from the group consisting of C₁-C₂ alkoxycarbonyl, 5-10-membered heteroaryl optionally substituted with one or more C₁-C₂ alkyl, one to four C₁-C₄ alkyl, C₃-C₇ cycloalkyl, and C₈-C₁₄ aryl;

C₈-C₁₀ aryl optionally substituted with one or two C₁-C₂ alkyl;

C₁-C₄ alkylcarbonyl;

or C₈-C₁₄ aryl-C₀-C₄ alkylene-O—C₀-C₄ alkyl, wherein each C₀-C₄ alkyl and each C₀-C₄ alkylene is optionally substituted with one to four C₁-C₄ alkyl;

R⁴ is independently selected from the group consisting of hydrogen, C₁-C₄ alkyl, C₁-C₄ alkoxyl, halogen, nitrile, —SO₂C₁-C₄, —SO₂NHC₁-C₄, and —C(═O)NHC₁-C₄;

n is 0, 1, 2, 3, or 4;

R⁵ is OH, —O (C₁-C₃)alkyl, halogen or hydrogen;

R⁶ is hydrogen, C₁-C₄ alkyl optionally substituted with 1 to 4 halogens, or C₃-C₇ cycloalkyl-C₀-C₄ alkyl;

R⁷ is hydrogen, C₁-C₈ alkyl optionally substituted with 1 to 4 halogens, or C₃-C₇ cycloalkyl-C₀-C₄ alkyl, wherein each C₀-C₄ is optionally substituted with one to four C₁-C₄ alkyl and,

R⁸ is hydrogen, C₁-C₈ alkyl optionally substituted with 1 to 4 halogens, or C₃-C₇ cycloalkyl-C₀-C₄ alkyl;

or optionally R⁷ and R⁸, together with the nitrogen to which they are attached, form a 4-, 5-, 6-, or 7-membered heterocyclic ring, wherein said heterocyclic ring is optionally substituted with one or two C₁-C₄ alkyl; and wherein one of the carbons of said heterocyclic ring that is separated by at least two atoms from said nitrogen in said heterocyclic ring is optionally replaced by O, S, NR⁹, or C═O, wherein R⁹ is hydrogen, C₁-C₈ alkyl optionally substituted with 1 to 4 halogens, or C₃-C₇ cycloalkyl-C₀-C₄ alkyl, and wherein each C₀-C₄ alkyl is optionally substituted with one to four C₁-C₄ alkyl.

A preferred embodiment of the invention includes those compounds of formula I wherein R⁷ and R⁸, together with the nitrogen to which they are attached, form a 4-, 5-, 6-, or 7-membered heterocyclic ring, wherein said heterocyclic ring is optionally substituted with one or two C₁-C₄ alkyl; and wherein one of the carbons of said heterocyclic ring that is separated by at least two atoms from said nitrogen in said heterocyclic ring is optionally replaced by O, S, NR⁹, or C═O, wherein R⁹ is hydrogen, C₁-C₈ alkyl optionally substituted with 1 to 4 halogens, or C₃-C₇ cycloalkyl-C₀-C₄ alkyl, and wherein each C₀-C₄ alkyl is optionally substituted with one to four C₁-C₄ alkyl.

A more preferred embodiment of the invention includes those compounds of formula I wherein R⁷ and R⁸, together with the nitrogen to which they are attached, form a 5- or 6-membered saturated heterocycle.

The most preferred embodiment of the invention includes those compounds of formula I wherein R⁷ and R⁸, together with the nitrogen to which they are attached, form a pyrrolidinyl group.

Another embodiment of the invention includes those compounds of formula I wherein R¹ is hydrogen; R⁴ and R⁵ are independently hydrogen or F; R⁵ is hydrogen or C₁-C₈ alkyl.

Another embodiment of the invention includes those compounds of formula I wherein R⁵ is H.

Another embodiment of the invention includes those compounds of formula I wherein R⁵ is F.

Another embodiment of the invention includes the cis cyclobutyl isomers of formula I.

Another embodiment of the invention includes the trans cyclobutyl isomers of formula I.

The most preferred embodiment of the present invention includes both the following cis and trans compounds of formula I:

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid dimethylamide;

[3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl-methanone;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethyl-methyl-amide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide;

{3-[3-Chloro-4-((R)-2-methyl-pyrrolidin-1-ylmethyl)-phenyl]-cyclobutyl}-pyrrolidin-1-yl-methanone;

3-(2,3-Dichloro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid dimethylamide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethylamide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid isobutyl-amide; and

(3-Aza-bicyclo[3.2.2]non-3-yl)-[3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-methanone.

[3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]pyrrolidin-1-yl-methanone;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid dimethylamide;

[3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutyl)-piperidin-1-yl-methanone;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanercarboxylic acid isobutyl-methyl-amide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid cyclopropylmethyl-amide;

[3-(3,5-Difluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutyl]-pyrrolidin-1-yl-methanone;

3-(2,6-Difluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid methylamide;

3-(5-Chloro-2-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid isobutyl-amide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid ethylamide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid methyl-(tetrahydro-pyran-4-ylmethyl)-amide;

3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide;

{3-[3-Chloro-4-((R)-2-methyl-pyrrolidin-1-ylmethyl)-phenyl]-3-fluoro-cyclobutyl}-pyrrolidin-1-yl-methanone;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid cyclopropylmethyl-methyl-amide;

3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid dimethylamide;

3-Fluoro-3-[3-fluoro-4-((S)-2-methyl-pyrrolidin-1-ylmethyl)-phenyl]-cyclobutanecarboxylic acid ethylamide;

[3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutyl]-(2,3-dihydro-5H-benzo[f][1,4]oxazepin-4-yl)-methanone;

3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethylamide;

3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethyl-methyl-amide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid methyl-(3-methyl-pyridin-2-ylmethyl)-amide;

3-Fluoro-3-[3-fluoro-4-((R)-2-methyl-pyrrolidin-1-ylmethyl)-phenyl]-cyclobutanecarboxylic acid ethylamide;

3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid isobutyl-amide;

[3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl-methanone;

[3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl-methanone; and

3-(2,3-Dichloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid dimethylamide.

This invention is also directed to pharmaceutical composition for treating a disorder or condition that may be treated by antagonizing histamine-3 receptors, the composition comprising a compound of formula I and optionally a pharmaceutically acceptable carrier.

This invention is also directed to a method of treatment of a disorder or condition that may be treated by antagonizing histamine-3 receptors, the method comprising administering to a mammal in need of such treatment a compound of formula I.

This invention is also directed to a method of treatment of a disorder or condition selected from the group consisting of depression, mood disorders, schizophrenia, anxiety disorders, cognitive disorders, Alzheimer's disease, attention-deficit disorder (ADD), attention-deficit hyperactivity disorder (ADHD), psychotic disorders, sleep disorders, obesity, dizziness, epilepsy, motion sickness, respiratory diseases, allergy, allergy-induced airway responses, allergic rhinitis, nasal congestion, allergic congestion, congestion, hypotension, cardiovascular disease, diseases of the GI tract, hyper and hypo motility and acidic secretion of the gastro-intestinal tract, the method comprising administering to a mammal in need of such treatment a compound of formula I.

This invention is also directed to a pharmaceutical composition for treating allergic rhinitis, nasal congestion or allergic congestion comprising: (a) an H3 receptor antagonist compound of formula I or a pharmaceutically acceptable salt thereof; (b) an H1 receptor antagonist or a pharmaceutically acceptable salt thereof; and (c) a pharmaceutically acceptable carrier; wherein the active ingredients (a) and (b) above are present in amounts that render the composition effective in treating allergy rhinitis, nasal congestion or allergic congestion.

This invention is also directed to a pharmaceutical composition for treating ADD, ADHD, depression, mood disorders, or cognitive disorders comprising: (a) an H3 receptor antagonist compound of Formula I or a pharmaceutically acceptable salt thereof; (b) a neurotransmitter re-uptake blocker or a pharmaceutically acceptable salt thereof; (c) a pharmaceutically acceptable carrier; wherein the active ingredients (a) and (b) above are present in amounts that render the composition effective in treating depression, mood disorders, and cognitive disorders.

This invention is also directed to a process for the preparation of a compound according to formula I, wherein the process comprises the step of reacting a compound of the formula 4.

with an organometallic reagent derived from a compound of formula 2,

followed by the direct amide formation to yield a compound of the formula I.

In the general formula I according to the present invention, when a radical is mono- or poly-substituted, said substituent(s) can be located at any desired position(s), unless otherwise stated. Also, when a radical is polysubstituted, said substituents can be identical or different, unless otherwise stated.

The histamine-3 (H3) receptor antagonists of the invention are useful for treating, in particular, ADD, ADHD, obesity, anxiety disorders and respiratory diseases. Respiratory diseases that may be treated by the present invention include adult respiratory distress syndrome, acute respiratory distress syndrome, bronchitis, chronic bronchitis, chronic obstructive pulmonary disease, cystic fibrosis, asthma, emphysema, rhinitis and chronic sinusitis.

The pharmaceutical composition and method of this invention may also be used for preventing a relapse in a disorder or condition described in the previous paragraphs. Preventing such relapse is accomplished by administering to a mammal in need of such prevention a compound of formula I as described above.

The disclosed compounds may also be used as part of a combination therapy, including their administration as separate entities or combined in a single delivery system, which employs an effective dose of a histamine H3 antagonist compound of general formula I and an effective dose of a histamine H1 antagonist, such as cetirizine (Zyrtec™), chlorpheniramine (Chlortrimeton™), loratidine (Claritin™), fexofenadine (Allegra™), or desloratadine (Clarinex™) for the treatment of allergic rhinitis, nasal congestion, and allergic congestion.

The disclosed compounds may also be used as part of a combination therapy, including their administration as separate entities or combined in a single delivery system, which employs an effective dose of a histamine H3 antagonist compound of general formula I and an effective dose of a neurotransmitter reuptake blocker. Examples of neurotransmitter reuptake blockers will include the serotonin-selective reuptake inhibitors (SSRI's) like sertraline (Zoloft™), fluoxetine (Prozac™), and paroxetine (Paxil™), or non-selective serotonin, dopamine or norepinephrine reuptake inhibitors for treating ADD, ADHD, depression, mood disorders, or cognitive disorders.

The compounds of the present invention may have optical centers and therefore may occur in different enantiomeric configurations. Formula I, as depicted above, includes all enantiomers, diastereomers, and other stereoisomers of the compounds depicted in structural formula I, as well as racemic and other mixtures thereof. Individual isomers can be obtained by known methods, such as optical resolution, optically selective reaction, or chromatographic separation in the preparation of the final product or its intermediate.

The present invention also includes isotopically labeled compounds, which are identical to those recited in formula I, but for the fact that one or more atom--s are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes that can be incorporated into compounds of the present invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, sulfur, fluorine and chlorine, such as ²H, ³H, ¹³C, ¹¹C, ¹⁴C, ¹⁵N, ¹⁸O, ¹⁷O, ¹⁵O, ³¹P, ³²P, ³⁵S, ¹⁸F, and ³⁶Cl, ¹²³I respectively. Compounds of the present invention and pharmaceutically acceptable salts of said compounds which contain the aforementioned isotopes and/or other isotopes of other atoms are within the scope of this invention. Certain isotopically labeled compounds of the present invention, for example those into which radioactive isotopes such as ³H and ¹⁴C are incorporated, are useful in drug and/or substrate tissue distribution assays. Tritiated, i.e., ³H, and carbon-14, i.e., ¹⁴C, isotopes are particularly preferred for their ease of preparation and detectability. Further, substitution with heavier isotopes such as deuterium, i.e., ²H, can afford certain therapeutic advantages resulting from greater metabolic stability, for example increased in vivo half-life or reduced dosage requirements and, hence, may be preferred in some circumstances.

Substitution with positron emitting isotopes, such as ¹¹C, ¹⁸F, ¹⁵O and ¹³N, can be useful in Positron Emission Topography (PET) studies for examining substrate receptor occupancy.

Anxiety disorders include, for example, generalized anxiety disorder, panic disorder, PTSD, and social anxiety disorder. Mood disorders include, for example, depressed mood, mixed anxiety and depressed mood, disturbance of conduct, and mixed disturbance of conduct and depressed mood. Cognitive disorders include, for example, in addition to ADHD, attention-deficit disorder (ADD) or other attention adjustment or Cognitive disorders due to general medical conditions. Psychotic disorders include, for example, schizoaffective disorders and schizophrenia, sleep disorders include, for example, narcolepsy and enuresis.

Examples of the disorders or conditions which may be treated by the compound, composition and method of this invention are also as follows: depression, including, for example, depression in cancer patients, depression in Parkinson's patients, post-myocardial infarction depression, depression in patients with human immunodeficiency virus (HIV), Subsyndromal Symptomatic depression, depression in infertile women, pediatric depression. major depression, single episode depression, recurrent depression, child abuse induced depression, post partum depression, DSM-IV major depression, treatment-refractory major depression, severe depression, psychotic depression, post-stroke depression, neuropathic pain, manic depressive illness, including manic depressive illness with mixed episodes and manic depressive illness with depressive episodes, seasonal affective disorder, bipolar depression BP I, bipolar depression BP II, or major depression with dysthymia; dysthymia; phobias, including, for example, agoraphobia, social phobia or simple phobias; eating disorders, including, for example, anorexia nervosa or bulimia nervosa; chemical dependencies including, for example, addictions to alcohol, cocaine, amphetamine and other psychostimulants, morphine, heroin and other opioid agonists, phenobarbital and other barbiturates, nicotine, diazepam, benzodiazepines and other psychoactive substances; Parkinson's diseases, including, for example, dementia in Parkinson's disease, neuroleptic-induced parkinsonism or tardive dyskinesias; headache, including, for example, headache associated with vascular disorders; withdrawal syndrome; age-associated learning and mental disorders, apathy, bipolar disorder, chronic fatigue syndrome, chronic or acute stress, conduct disorder; cyclothymic disorder; somatoform disorders such as somatization disorder, conversion disorder, pain disorder, hypochondriasis, body dysmorphic disorder, undifferentiated disorder, and somatoform NOS; incontinence; inhalation disorders; intoxication disorders; mania; oppositional defiant disorder; peripheral neuropathy; post traumatic stress disorder; late luteal phase dysphoric disorder; specific developmental disorders; SSRI “poop out” syndrome, or a patient's failure to maintain a satisfactory response to SSRI therapy after an initial period of satisfactory response; and tic disorders including Tourette's disease.

As an example, the mammal in need of the treatment or prevention may be a human. As another example, the mammal in need of the treatment or prevention may be a mammal other than a human.

Pharmaceutically acceptable salts of the compounds of formula I include the acid addition and base salts thereof.

Suitable acid addition salts are formed from acids which form non-toxic salts. Examples include the acetate, aspartate, benzoate, besylate, bicarbonate/carbonate, bisulphate/sulphate, borate, camsylate, citrate, edisylate, esylate, formate, fumarate, gluceptate, gluconate, glucuronate, hexafluorophosphate, hibenzate, hydrochloride/chloride. hydrobromide/bromide, hydroiodide/iodide, isethionate, lactate, malate, maleate, malonate, mesylate, methylsulphate, naphthylate, 2-napsylate, nicotinate, nitrate, orotate, oxalate, palmitate, pamoate, phosphate/hydrogen phosphate/dihydrogen phosphate, saccharate, stearate, succinate, tartrate, tosylate and trifluoroacetate salts.

Suitable base salts are formed from bases which form non-toxic salts. Examples include the aluminium, arginine, benzathine, calcium, choline, diethylamine, diolamine, glycine, lysine, magnesium, meglumine, olamine, potassium, sodium, tromethamine and zinc salts.

Hemisalts of acids and bases may also be formed, for example, hemisulphate and hemicalcium salts.

For a review on suitable salts, see “Handbook of Pharmaceutical Salts: Properties, Selection, and Use” by Stahl and Wermuth (Wiley-VCH, Weinheim, Germany, 2002).

The compounds of the invention may exist in both unsolvated and solvated forms. The term 'solvate' is used herein to describe a molecular complex comprising the compound of the invention and a stoichiometric amount of one or more pharmaceutically acceptable solvent molecules, for example, ethanol. The term 'hydrate' is employed when said solvent is water.

Included within the scope of the invention are complexes such as clathrates, drug-host inclusion complexes wherein, in contrast to the aforementioned solvates, the drug and host are present in stoichiometric or non-stoichiometric amounts. Also included are complexes of the drug containing two or more organic and/or inorganic components which may be in stoichiometric or non-stoichiometric amounts. The resulting complexes may be ionised, partially ionised, or non-ionised. For a review of such complexes, see J Pharm Sci. 64 (8), 1269-1288 by Haleblian (August 1975).

Hereinafter all references to compounds of formula I include references to salts, solvates and complexes thereof and to solvates and complexes of salts thereof.

The compounds of the invention include compounds of formula I as hereinbefore defined, including all polymorphs and crystal habits thereof, and isomers thereof (including optical, geometric and tautomeric isomers) as hereinafter defined and isotopically-labeled compounds of formula I.

Compounds of formula I containing one or more asymmetric carbon atoms can exist as two or more stereoisomers. Where structural isomers are interconvertible via a low energy barrier, tautomeric isomerism ('tautomerism') can occur. This can take the form of proton tautomerism in compounds of formula I containing, for example, an imino, keto, or oxime group, or so-called valence tautomerism in compounds which contain an aromatic moiety. It follows that a single compound may exhibit more than one type of isomerism.

Included within the scope of the present invention are all stereoisomers, geometric isomers and tautomeric forms of the compounds of formula I, including compounds exhibiting more than one type of isomerism, and mixtures of one or more thereof. Also included are acid addition or base salts wherein the counterion is optically active, for example, d-lactate or l-lysine, or racemic, for example, dl-tartrate or dl-arginine.

Unless otherwise indicated, the term "halo", as used herein, includes fluoro, chloro, bromo and iodo.

Unless otherwise indicated, the term "(C₁-C₄)alkyl", as used herein, includes saturated, straight-chain or branched hydrocarbon group having from 1 to 4 carbon atoms and includes for example methyl, ethyl, propyl, i-propyl, n-butyl, i-butyl, sec-butyl and t-butyl. This also applies if the alkyl group carries substituents or is a substituent for another group, e.g. in —O—(C₁-C₄)alkyl and —C(O)(C₁-C₄)alkyl.

Unless otherwise indicated, the term "(C₁-C₄)alkoxy", as used herein, includes straight-chain and branched alkoxy groups and includes for example methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, i-butoxy, sec-butoxy and t-butoxy.

Unless otherwise indicated, the term "(C₂-C₆)alkylene", as used herein, includes a divalent radical derived from straight-chain or branched alkane containing from 2 to 6 carbon atoms. Examples of (C₂-C₆)alkylene radicals are methylene, ethylene (1,2-ethylene or 1,1-ethylene), trimethylene (1,3-propylene), tetramethylene (1,4-butylene), pentamethylene and hexamethylene.

Unless otherwise indicated, the term "(C₃-C+₈)cycloalkyl", as used herein includes saturated monocyclic carbocyclic group having 3 to 6 carbon atoms and includes for example cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.

Unless otherwise indicated, the term "saturated heterocycle". as used herein, includes a saturated monocyclic groups having 4 to 7 ring members, which contains 1 nitrogen atom. Examples of saturated heterocycles are azetidinyl, pyrrolidinyl and piperidinyl.

Unless otherwise indicated, the terms "heteroaromatic" as used herein, includes monocyclic or bicyclic heteroaromatic groups having 5 to 9 and 5 to 10 ring members respectively, which contain 1, 2, 3 or 4 heteroatom(s) selected from nitrogen, oxygen and sulphur. The heteroaromatic group can be unsubstituted, monosubstituted or disubstituted. Examples of heteroaryl groups include, but are not limited to thiophenyl, furanyl, pyrrolyl, pyrazolyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, thiazolyl, oxadiazolyl, thiadiazolyl, tetrazolyl, pyranyl, pyridinyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, thiadiazinyl, isobenzofuranyl, benzofuranyl, chromenyl, indolizinyl, isoindolyl, indolyl, indazolyl, purinyl, quinolinyl, isoquinolyl, cinnolinyl, phthalazinyl, naphthyridinyl, quinazolinyl, quinoxalinyl, benzoxazolyl, benzothiazolyl, benzimidazolyl, benzofuranyl, benzothiophenyl, pyrrolopyrazinyl, pyrrolopyridinyl, and imidazopyridinyl.

DETAILED DESCRIPTION OF THE INVENTION

The compound of formula I according to the invention may be prepared by the general procedure shown in Scheme 1.

In Scheme 1, compounds of the formula (I) are prepared as follows.

Step A:

An aryl bromide of the general structure 2 is converted to an organometallic reagent such as: an organolithium, organomagnesium halide, organocerium, organotitanium, organozinc, organocopper, or organoaluminum reagent. An organomagnesium halide (Grignard reagent) or organolithium reagent is preferred. For example, the organolithium reagent can be prepared by reaction of aryl bromide (2) with nBuLi. The reaction is typically effected in a reaction-inert-solvent, such as tetrahydrofuran, at a temperature between about −78° C. and about room temperature. To this organolithium reagent, at about −78° C., is added a solution of 3-oxocyclobutanecarboxylic acid (J. Org. Chem. 1988, 53, 3841 and J. Org. Chem. 1996, 61, 2174), pre-cooled to −78° C., where preferred solvent is tetrahydrofuran. After complete addition the reaction is allowed to slowly warm to room temperature to yield a compound of the general structure 3.

Step B:

Intermediate of the general structure 3 may be reacted with a primary or secondary amine of general formula HNR¹R², where R¹ and R² are as defined in the specification amine, in the presence of a coupling reagent such as dicyclohexyl carbodiimide, carbonyl diimidazole, tripropylphosphonic anhydride, alkyl chloroformate, bis(2-oxo-3-oxazolidinyl)phosphinic chloride, benzotriazol-1-yloxy-tris(dimethylamino)phosphonium hexafluorophosphate, or any other such standard literature reagents in the presence of a trialkyl amine base, such as triethyl amine or diisopropylethyl amine, wherein tripropylphosphonic anhydride and triethylamine are a preferred combination in reaction-inert-solvent, where ethyl acetate is preferred, from −78° C. to 40° C., where room temperature is preferred, to afford the N-acylated compounds of the general structure 4, a compound of Formula I.

Step C:

Elimination of the benzyl alcohol (4) is accomplished by reaction of (4) with an acid preferably With trifluoroacetic acid either neat or in a reaction-inert-solvent, such as methylene chloride or 1,2-dichloroethane at a reaction temperature from about room temperature to the reflux temperature of the solvent employed, where about 75° C. is the preferred reaction temperature, to yield a compound with the general structure 5.

Step D:

Reduction of the cyclobutene, (5) can be accomplished by reaction of (5) in a reaction-inert-solvent, where preferred solvents are ethyl alcohol and ethyl acetate. The reduction can be accomplished using hydrogen gas at about 45 psi and a catalyst, where preferred hydrogenation catalysts are Wilkinson's catalyst [chlorotris(triphenylphosphine)rhodium(I)], or palladium, 5-10 wt % on activated carbon to give (6), a compound of the general Formula I.

Step E:

Conversion of benzyl amines of the general structure (6,) to benzyl chlorides of the general structure (7) is accomplished using condition described in the literature, for example (Nevill, C. R.; Fuchs, P. L.; SYNCAV; Synth. Commun.; EN; 20; 5 1990; 761-772). Reaction of (6) with ethyl chloroformate in a reaction-inert-solvent, where 1,2-dichloroethane, or methylene chloride are preferred at a reaction temperature from about −78° C. to room temperature, where room temperature is preferred, gives benzyl chlorides of the general structure (7).

Step F:

Reaction of the benzyl chloride (7) with a primary or secondary amine of general formula HNR¹R², where R¹ and R² are as defined in the specification amine, in a reaction-inert-solvent, where 1,2-dichlorethane or methylene chloride are preferred, in the presence of a tertiary amine base, where triethylamine is preferred, at a reaction temperature from About room temperature to the reflux temperature of the solvent employed, about 55° C. is preferred gives (8), a compound of the general formula I.

In Scheme 2, compounds of the formula (I) are prepared as follows.

Step G:

Fisher etherification can be accomplished using standard conditions that appear in the literature and known to those skilled in the art For example: reaction of hydroxyl (4) with an alkyl halide, such as: alkyl chloride, alkyl bromide, or alkyl iodide in the presence of a base where NaH is preferred, and in the presence of Nal or NaBr, in a reaction-inert-solvent, where dimethyl formamide is preferred, at a reaction temperature of room temperature to 100° C., where 65° C. is preferred gives (8), a compound of the formula I.

Step E:

(See Step E above)

Step F:

(See Step F above)

In Scheme 3, compounds of the formula (I) are prepared as follows.

Step H:

Reaction of a compound of the general structure (4) with a fluorinating reagent gives compounds of the general formula 13 Several reagents are available for conversion of alcohols to alkyl fluorides, for example, Caldwell, Charles G; et al (Bioorg. Med. Chem. Lett.; EN; 14; 5; 2004; 1265-1268) utilizes BAST. Other examples in the literature utilize DAST for the direct conversion of alcohols to alkyl fluorides. Reaction of hydroxyl (3) with bis(2-methoxyethyl)aminosulfur trifluoride in a reaction-inert-solvent, where methylene chloride or tetrahydrofuran is preferred, at a reaction temperature from about −78° C. to room temperature gives (11), a compound of the formula I.

Step E:

(see Step E above)

Step F:

(see Step F above)

Exemplary compounds of formula I in accordance with the present invention are the following:

N-Methyl-2-pyridin-3-yl-N-[3-(4-pyrrolidin-1-ylmethyl-phenoxy)-cyclobutylmethyl]-acetamide;

[3-Hydroxy-3-(4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-morpholin-4-yl-methanone;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid methylamide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid methylamide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid dimethylamide;

[3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutyl]-piperidin-1-yl-methanone;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid isobutyl-methyl-amide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid cyclopropylmethyl-amide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid methyl-(tetrahydro-pyran-4-ylmethyl)-amide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid cyclopropylmethyl-methyl-amide;

[3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutyl]-(2,3-dihydro-5H-benzo[f][1,4]oxazepin-4-yl)-methanone;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid methyl-(3-methyl-pyridin-2-ylmethyl)-amide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid dimethylamide;

[3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutyl]-pyrrolidin-1-yl-methanone;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid isobutyl-amide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid ethylamide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclabutanecarboxylic acid ethyl-methyl-amide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxyic acid dimethylamide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid dimethylamide;

[3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl-methanone;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid isobutyl-amide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethylamide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethyl-methyl-amide;

[3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cylobutyl]-piperidin-1-yl-methanone;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid isobutyl-methyl-amide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid cyclopropylmethyl-amide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cycolobutanecarbaxyic acid methylamide,

3-(2,6-Difluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acd methylamide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid methyl-(tetrahydro-pyran-4-ylmethyl)-amide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid cyclopropylmethyl-methyl-amide;

[3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutyl]-(2,3-dihydro-5H-benzo[f][1,4]oxazepin-4-yl)-methanone;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid methyl-(3-methyl-pyridin-2-ylmethyl)-amide;

[3-(3,5-Difluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutyl]-pyrrolidin-1-yl-methanone;

[3-(3 5-Difluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cycobutyl]-pyrrolidin-1-yl-methanone;

3-(5-Chloro-2-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid isobutyl-amide;

3-(5-Chloro-2-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid isobutyl-amide:

3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide;

3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid dimethylamide;

3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethylamide;

3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylio acid ethyl-methyl-amide;

3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxyldc acid isobutyl-amide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-methoxy-cyclobutanecarboxylic acid ethyl-methyl-amide;

[3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl-methanone;

3-(2,3-Dichloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid dimethylamide;

3-(2,3-Dichloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid dimethylamide;

{3-[3-Chloro-4-((R)-2-methyl-pyrrolidin-1-ylmethyl)-phenyl]-3-hydroxyy-cyclobtutyl}-pyrrolidin-1-yl-methanone;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid ethylamide;

3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid ethylanmide;

{3-[3-Chloro-4-((R)-2-methyl-pyrrolidin-1-ylmethyl)-phenyl]-3-fluoro-cyolobutyl}-pyrrolidi-1-yl-methanone;

{3-[3-Chloro-4-((R)-2-methyl-pyrrolidin-1-ylmethyl)-phenyl]-cyclobutyl}-pyrrolidin-1-yl-methanone;

3-(2,3-Dichloro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid dimethylamide;

3-Fluoro-3-[3-fluoro-4-((S)-2-methyl-pyrrolidin-1-ylmethyl)-phenyl]-cyclobutanecarboxylic acid ethylamide;

3-Fluoro-3-[3-fluoro-4-((R)-2-methyl-pyrrolidin-1-ylmethyl)-phenyl]-cyclobutanecarboxylic acid ethylamide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethylamide;

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid isobutyl-amide; and

3-aza-bicyclo[3.2.2]nonan-3-yl(3-3-fluoro-4-(pyrrolidin-1-ylmethyl)phenyl)-3-hydroxycyclobutyl)methanone.

In the examples below the following terms are intended to have the following, general meaning:

BAST: [BIS(2-METHOXYETHYL)AMlNO]SULFUR TRIFLUORIDE

Deoxo-Fluor: [BIS(2-METHOXYETHYL)AMINO]SULFUR TRIFLUORIDE

T₃P: 2,4,6-tripropyl-1,3,5,2,4,6-trioxatriphosphorinane-2,4,6-trioxide

DIPEA: diisopropylethylamine DMF: dimethyformamide

MgSO₄: magnesium sulfate DMA: dimethyl acetamide

LRMS: low resolution mass spectrometry ° C.: degrees Celsius

calcd: calculated d: day(s); doublet (spectral) DCE: 1,2-dichloroethane

EtOAc: ethyl acetate g: grams

hr: hours Hz: hertz

J: coupling constant (in NMR) L: liter(s)

LAH: lithium aluminum hydride MHz: megahertz

m/z: mass to charge ratio (mass spectrometry) Min: minute(s)

obsd: observed PPTs: pyridinium p-toluenesulfonate

TsO: p-toluenesulfonate Rf: retention factor (in chromatography) Rt: retention time (in chromatography) rt: room temperature

s: singlet (NMR); second(s) t: triplet

TFA: trifluoroacetic acid TFAA: trifluoroacetic anhydride

THF: tetrahydrofuran TLC: thin layer chromatography

Ts: tosyl, p-toluenesulfonyl TsOH: p-toluenesulfonic acid

apt: apparent triplet

Solvents were purchased and used without purification. Yields were calculated for material judged homogenous by thin layer chromatography and NMR. Thin layer chromatography was performed on Merck Kieselgel 60 F. 254 plates eluting with the solvents indicated, visualized by a 254 nm UV lamp, and stained with either an aqueous KMnO₄ solution or an ethanolic solution of 12-molybdophosphoric acid. Flash column chromatography unless otherwise stated, was performed with using either pre-packed Biotage™ or ISCO™ columns using the size indicated. Nuclear magnetic resonance (NMR) spectra were acquired on a Unity 400 or 500 at 400 MHz or 500 MHz for ¹H, respectively, and 100 MHz or 125 MHz for ¹³C NMR, respectively. Chemical shifts for proton ¹H NMR spectra are reported in parts per million relative to the singlet of CDCl₃ at 7.24 ppm. Chemical shifts for ¹³C NMR spectra are reported in parts per million downfield relative to the centerline of the triplet of CDCL₃ at 77.0 ppm. Mass spectra analyses were performed on a APCI Gilson 215, micromass ZMD (50% Acetonitrile/50% water) spectrometer.

HPLC was performed according to the following methods:

Method A: Preparative conditions (Waters 600 & Waters 2767 Sample Manager); Column: Waters Symmetry C₁₈, 5 μm, 30×150 mm steel column, part #WAT248000, serial # M12921A01; solvent A—0.1% Trifluoroacetic acid/water; solvent B—Acetonitrile; volume of injection: 850 μL; time 0.0, 100% solvent A, 0% solvent B, flow 20; time 2.0, 100% solvent A, 0% solvent B, flow 20; time 12.0, 0% solvent A, 100% solvent B, flow 20; time 15.0, 0% solvent A, 100% solvent B, flow 20; time 15.1, 100% solvent A, 0% solvent B, flow 20; time 20.0, 100% solvent A, 0% solvent B, flow 20.

Mass spectral (micromassZO) conditions; Capillary(kV): 3.0 Cone (V): 20; Extractor (V): 3.0; RF Lens (V): 0.5; Source temp. (° C.): 120; Desolvation temp. (° C.): 360; Desolvation gas flow (L/hr): 450; Cone gas flow (L/hr): 150; LM Resolution: 15; HM Resolution: 15; Ion Energy: 0.2; Multiplier: 550.

Splitter; Acurate by LC Packings, 1/10,000; Upchurch needle valve setting: 14; Make up pump (Waters 515) Flow (ml/min.): 1.

PDA (Waters 996) Settings, Start/End wavelength (nm): 200/600; Resolution: 1.2; Sample Rate: 1; Channels: TIC, 254 nm and 220 nm.

Method B: Preparative conditions (Waters 600 & Waters 2767 Sample Manager); Column: Waters Xterra PrepMS C₁₈ column, 5 μm, 30×150 mm steel column, part # 186001120, serial # T22881T 09; solvent A—0.1% Trifluoroacetic acid/water, solvent B—Acetonitrile: volume of injection: 1050 μL; time 0.0, 100% solvent A, 0% solvent B, flow 20; time 2.0, 100% solvent A, 0% solvent B, flow 20; time 12.0, 0% solvent A, 100% solvent B, flow 20; time 14.0, 0% solvent A, 100% solvent B, flow 20; time 14.1, 100% solvent A, 0% solvent B, flow 20; time 19.1, 100% solvent A, 0% solvent B, flow 20.

Mass spectral (micromassZO) conditions; Capillary(kV): 3.0; Cone (V): 20; Extractor (V): 3.0; RF Lens (V): 0.5; Source temp. (° C.): 120; Desolvation temp. (° C.): 360; Desolvation gas flow (L/hr): 450; Cone gas flow (L/hr): 150; LM Resolution: 15; HM Resolution: 15; Ion Energy: 0.2; Multiplier: 550.

Splitter: Acurate by LC Packings, 1/10,000: Upchurch needle valve setting: 14; Make up pump (Waters 515) Flow (ml/min.): 1.

PDA (Waters 996) Settings; Start/End wavelength (nm): 200/600; Resolution: 1.2; Sample Rate: 1; Channels: TIC, 254 nm and 220 nm.

Method C: Preparative conditions (Waters 600 & Waters 2767 Sample Manager); Column: Waters Symmetry C₁₈, 5 μm, 30×150 mm steel column, pail #WAT248000, serial # M12921A01: solvent A—0.1% Trifluoroacetic acid/water; solvent B—Acetonitrile; volume of injection: 850 μL; time 0.0, 90% solvent A, 10% solvent B, flow 20; time 10.0, 0% solvent A, 100% solvent B, flow 20; time 12.0, 0% solvent A, 100% solvent B, flow 20.

Mass spectral (micromassZO) conditions: Capillary(kV): 3.0: Cone (V): 20; Extractor (V): 3.0; RF Lens (V): 0.5; Source temp. (° C.): 120; Desolvation temp. (° C.): 360; Desolvation gas flow (L/hr): 450; Cone gas flow (L/hr): 150, LM Resolution: 15; HM Resolution: 15; Ion Energy: 0.2; Multiplier: 550.

Splitter; Acurate by LC Packings, 1/10,000; Upchurch needle valve setting: 14; Make up pump (Waters 515) Flow (ml/min.): 1.

PDA (Waters 996) Settings; Start/End wavelength (non): 200/600; Resolution: 1.2; Sample Rate: 1; Channels: TIC, 254 nm and 220 nm.

The following intermediates may be prepared by the procedures shown:

Intermediate 1

1-(2,3-Dichlorobenzyl)pyrrolidine

NaHB(OAc)₃ (15.1 g, 0.0714 mmol) was added under vigorous stirring in portions to a solution of 2,3-dichlorobenzaldehyde (10 g, 0.057 mmol) and pyrrolidine (5.97 mL, 0.0714 mmol in CH₂Cl₂ (200 mL). The reaction mixture was vigorously stirred overnight. Then 5 N NaOH (50 mL) was added, and the layers were separated. The product was extracted from the aqueous layer with CH₂Cl₂ (2×50 mL). The combined extracts were washed with 5 N NaOH (50 mL), water, brine, dried with anhydrous Na₂SO₄, and evaporated. The residue was distilled in vacuum to give title compound (10.5 g, 90%) as a colorless liquid (bp 80-84° C./0.5 mmHg). LC/MS data: 229.9, 230.9, 231.9 (M+H) (calculated for C₁₁H₁₃Cl₂N 230.14). ¹H NMR data (DMSO-d6): δ7.52 (dd, 1H, J1=1.5 Hz, J2=7.8 Hz), 7.46 (dd, 1H, J1=1.5 Hz, J2=7.8 Hz), 7.33 (t, 1H, J=7.8 Hz), 3.70 (s, 2H), 2.46-2.52 (m, 4H), 1.66-1.75 (m, 4H).

EXAMPLE 1

3-[2,3-Dichloro-4-(pyrrolidin-1-ylmethyl)phenyl]-3-hydroxy-N,N-dimethylcyclobutanecarboxamide

A 1.3 M solution of s-BuLi in cyclohexane (3.7 mL, 4.8 mmol) was added over 5 min to a solution of Intermediate 1, 1-(2,3-dichlorobenzyl)pyrrolidine (1.0 g, 4.4 mmol) and TMEDA (0.73 ml, 4.8 mmol) in absolute THE (10 ml) in a flow of argon at −90 to −100° C. The reaction mixture was stirred at −85 to −90° C. for 30 min. Then a solution of 3-oxocyclobutanecarboxylic acid (250 mg, 2.2 mmol), (J. Org. Chem. 1988, 53, 3841 and J. Org. Chem. 1996, 61, 2174) in THF (2 mL) was added drop wise over 2 min at −100° C. The mixture was then warmed to 0° C. for 30 min and evaporated to dryness. The residue was dissolved in DMF (10 mL), and dimethylamine hydrochloride (410 mg, 5.0 mmol) was added. Then BOP (1.3 g, 3.0 mmol) was added in portions under cooling in an ice bath. The reaction mixture was stirred for 16 h at room temperature. The disappearance of the starting hydroxy acid was monitored by LC/MS. The reaction mass was evaporated to dryness under 1 mmHg. Water (10 mL), Et₂O (15 mL), and a saturated solution of K₂CO₃ (5 mL) were added. The layers were separated, and the aqueous one was subjected to extraction with Et₂O (2×20 mL). The combined organic layer was dried with Na₂SO₄, and evaporated. The residue was purified by chromatography (30 mL of silica gel 63/100 μm, CHCl₃MeOH 100:0→90:10). The product containing fractions were collected, concentrated under reduced pressure to yield the title compound (0.30 g, 37%). LC/MS data, 371.0, 372.0, 373.0 (M+H) (calculated for C₁₈H₂₄Cl₂N₂O₂ 371.31); ¹H NMR data (DMSO-d6): δ 7.57 (d, 1H, ArH, J=8.0 Hz), 7.43 (d, 1H, ArH, J=8.0 Hz), 5.59 (s, 1H, OH), 3.71 (s, 2H, CH2Ar), 2.88-2.97 (m, 2H), 2.86 (s, 3H NMe), 2.82 (s, 3H, NMe), 2.67-2.78 (m, 1H), 2.50-2.57 (m, 6H+DMSO), 1.67-1.77 (m, 4H). The HCl salt was made using HCl, ether. A 8 mL screw cap vial was charged with 3-[2,3-dichloro-4-(pyrrolidin-1-ylmethyl)phenyl]-3-hydroxy-N,N-dimethylcyclobutanecarboxamide (60 mg, 0.161 mmol) and 0.5 mL of MeOH. Then 0.2 mL of 2M HCl in ether was added, evaporated and dried to give oil, which was redissolved in 1 mL of DCM, evaporated and dried to give 62 mg of white hygroscopic solid of HCl salt. LCMS (M+H): 371.1; ¹H NMR (300 MHz, DMSO-d₈): δ 10.57 (br. s, 1H), 7.80 (d, J=8.1 Hz, 1H), 7.73 (d, J=8.2 Hz, 1H), 4.57 (d, J=5.64 Hz, 2H), 3.50-3.70 (m, 3H), 3.40-3.50 (m, 3H), 2.90-2.97 (m, 2H), 2.86 (s, 3H), 2.83 (s, 3H), 2.69-2.75 (m, 1H), 2.03-2.07 (m, 2H), 1.80-1.94 (m, 2H).

Intermediate 2

3-[2,3-dichloro-4-(pyrrolidin-1-ylmethyl)phenyl]-N,N-dimethylcyclobut-2-ene-1-carboxamide, trifluoroacetate

A solution of 3-[2,3-dichloro-4-(pyrrolidin-1-ylmethyl)phenyl]-3-hydroxy-N,N-dimethylcyclobutanecarboxamide (250 mg, 0.673 mmol) and TFA (1.04 ml, 13.5 mmol) in 5 ml of DCE was refluxed under argon for 6 h, then additional amount of TFA (1.04 ml, 13.5 mmol) was added and the mixture was refluxed for 20 h. The mixture was evaporated to dryness. According to LCMS data the reaction mixture contained up to 70% of the title compound (353, 354, 355 (M+H) (calculated for C₁₈H₂₂Cl2N₂O 353.29)). The mixture was used for the next step without additional purification.

EXAMPLE 2

trans-3-[2,3-Dichloro-4-(pyrrolidin-1-ylmethyl)phenyl]-N,N-dimethylcyclobutanecarboxamide, hydrochloride

To a solution of intermediate 2, 3-[2,3-dichloro-4-(pyrrolidin-1-ylmethyl)phenyl]-N,N-dimethylcyclobut-2-ene-1-carboxamide, trifluoroacetate (0.673 mmol) in 5 mL of ethanol was added chlorotris(triphenylphosphine)rhodium(I) (63 mg, 0.0673 mmol). The mixture was hydrogenated (40 psi H₂, 50%) for 3 h; the reaction was monitored by LCMS. The mixture was evaporated to dryness, then 5 ml of 1 N HCl was added to the residue and the solution was extracted with ethyl acetate (2×5 ml), the organic layers were discarded. 10N NaOH (1 mL) was added to the water layer and the water solution was extracted with ethyl acetate (3×5 mL). The organic layers were dried and evaporated in vacuum. The residue was purified by chromatography (SiO₂ 63/100 μm, 10 g, CHCl₃/hexane 80:20→100:0, CHCl₃/MeOH 100:0→90:10). Fractions containing the product were evaporated. The residue was dissolved in 2 ml of ether and 0.1 mL of 4N HCl/dioxane was added under stirring. The mixture was evaporated; the residue was dried in vacuum to afford the title compound (74 mg, 28%) as a white solid. LCMS data; 355, 356, and 357 (M+H)⁺ (calculated for C₁₈H₂₄Cl₂N₂O 355.31). ¹H NMR data (CD₃OD). δ7.69 (d, 1H, J=8.1 Hz), 7.58 (d, 1H, J=8.1 Hz), 4.62 (s, 2H), 3.88-3.98 (m, 1H), 3.54-3.63 (m, 2H), 3.35-3.45 (m, 1H), 3.25-3.34 (m, 2H+MeOH), 2.99 (s, 6H), 2.71-2.79 (m, 2H), 2.42-2.52 (m, 2H), 2.16-2.27 (m, 2H), 1.98-2.12 (m, 2H).

EXAMPLE 3 3-[2,3-dichloro-4-(pyrrolidin-1-ylmethyl)phenyl]-3-fluoro-N,N-dimethylcyclobutanecarboxamide hydrochloride

An 8 mL screw cap vial, equipped with a magnetic stirring bar and septum cap, was charged with Deoxo-Fluor (Aldrich, 85.5 mg, 0.387 mmol) and 3 mL of anhydrous DCM under nitrogen. Then the mixture was cooled to −75° C. and a solution of Example 1,3-[2,3-dichloro-4-(pyrrolidin-1-ylmethyl)phenyl]-3-hydroxy-N,N-dimethylcyclobutanecarboxamide (130 mg, 0.350 mmol) in 2 mL of anhydrous DCM was added. The mixture was stirred for 1 h at −75° C., then sampled for LCMS, which showed 60% conversion. Then another Deoxo-Fluor (Aldrich, 85.5 mg, 0.387 mmol) was added, stirred at −75° C. for 10 min, warmed to 0° C. and quenched with sat. Na₂CO₃ (2 mL). LCMS showed complete conversion. Then 1 mL of 2N NaOH was added and the DCM layer was separated, dried over Na₂SO₄, and evaporated. The crude oil was purified by column (from DCM 99%, NH₄OH 1% to DCM 98%, MeOH 1%, NH₄OH 1%, Rf=0.51 in DCM 99%, NH₄OH 1%) to give 117 mg (90%) of the product as colorless oil. This material was dissolved in 0.5 mL of MeOH, then 0.3 mL of 2M HCl in ether was added, evaporated and dried to give oil, which was redissolved in 1 mL of DCM, evaporated and dried to give the title compound (118 mg) of a white hygroscopic solid, HCl salt. LCMS (M+H ): 373.3; ¹H NMR (300 MHz, DMSO-d₆), δ 10.27 (br s, 1H), 7.81 (d, J=7.71 Hz, 1H), 7.65 (dd, J1=8.1 Hz, J2=2.07 Hz, 1H), 4.59 (d, J=5.64 Hz, 2H), 3.62-3.71 (m, 6H), 3.45-3.50 (m, 2H), 2.95 (s, 3H), 2.85-2.88 (m, 1H), 2.80 (s, 3H), 2.00-2.10 (m, 2H), 1.80-1.91 (m, 2H).

Intermediate 3

4-Bromo-1-(bromomethyl)-2-chlorobenzene

4-Bromo-2-chloro-1-methylbenzene (CAS 89794-02-5, 30 g, 0.15 mol) and N-bromosuccinimide (26 g, 0.15 mol) were mixed in CCl₄ (300 mL). Azobis(2-methylpropionitrile) (˜0.3 g) was added in portions under vigorous stirring and refluxing. The mixture was refluxed for 30 min and cooled. The precipitate was filtered off and discarded The filtrate was evaporated. The residue was distilled at 1 mmHg, bp 75° C. to give the title compound (28 g, 65%). ¹H NMR data (CDCl₃): δ 7.57 (d, J=1.9 Hz, 1H ArH), 7.39 (dd, 1H, J₁=1.9 Hz, J₂=8.1 Hz, ArH), 7.31 (d, 1H, J=1.9 Hz, ArH), 4.53 (s, 2H, ArCH₂).

Intermediate 4

(2R)-1-(4-bromo-2-chlorobenzyl)-2-methylpyrrolidine

Intermediate 3, 4-bromo-1-(bromomethyl)-2-chlorobenzene (15.4 g, 55 mmol) was added to a mixture of (2R)-2-methylpyrrolidine HBr (9.0 g, 55 mmol), potassium carbonate (18 g, 130 mmol), and 150 mL of dimethylformamide under ice cooling. The mixture was allowed to reach room temperature, and the stirring was continued overnight. The mixture was evaporated. Water (400 mL) was added followed by addition of 5M NaHSO₄ to attain pH˜2. The organic layer was separated. The aqueous phase was extracted with Et₂O (2×200 mL). The organic layers were discarded. The aqueous fraction was alkalized with K₂CO₃ to pH˜12 and subjected to extraction with Et2O (2×300 mL). The organic layer was washed with brine, dried over anhydrous Na₂SO₄ (100 g), and evaporated in vacuo. The residue was distilled at 1 mmHg, bp 95° C. to give the title compound (12.25 g, 79%). LC/MS data: 289.9 and 287.9 (M)⁺ (calculated for C₁₂H₁₅BrClN 288.6). 1H NMR data (DMSO-d6): δ 7.66 (d, 1H, J=1.9 Hz, Ar—H); 7.52 (dd, 1H, J₁=1.9 Hz, J₂=8.0 Hz, Ar—H), 7.43 (d, 1H, J=8.1 Hz, Ar—H), 3.91 (d, 1H, J=14.4 Hz), 2.28 (d, 2H, J=8.5 Hz), 2.78-2.85 (m, 1H) 2.42-2.49 (m, 1H); 2.11 (dd, 1H, J₁=8.8 Hz, J₂=17.6 Hz); 1.87-1.97 (m, 1H); 1.57-1.67 (m, 2H); 1.27-1.39 (m, 1H); 1.08 (d, 3H, J=5.9 Hz).

Intermediate 5

Pyrrolidine Hydrochloride

4N HCl/dioxane (70.5 mL) was added to a solution of pyrrolidine (20 g, 0.28 mol) in dioxane (20 ml). The reaction mixture was evaporated. The residue was recrystallized from Et₂O, separated by filtration, washed with ether, and dried to give the title compound (28.5 g, 96%) as white crystals. ¹H NMR data (DMSO-d6): δ 9.40 (br.s, 2H, NH⁺); 3.00-3.13 (m, 4H); 1.77-1.85 (m, 4H).

EXAMPLE 4 1-(3-Chloro-4-{[(2R)-2-methylpyrrolidin-1-yl]methyl}phenyl)-3-(pyrrolidin-1- ylcarbonyl)cyclobutanol

A 2.7 M solution of n-BuLi in heptane (3.6 mL, 9.6 mmol) was added for 5 min to a solution of intermediate 4, (2R)-1-(4-bromo-2-chlorobenzyl)-2-methylpyrrolidine. (2.52 g, 8.8 mmol) in absolute THF (20 ml) in a flow of argon at −78 to −80° C. The reaction mixture was stirred at −78 to −80° C. for 15 min. Then a solution of 3-oxocyclobutanecarboxylic acid (500 mg, 4.4 mmol) in absolute THF (4 mL) was added drop wise over 5 min at −80° C. The mixture was warmed to 0° C. for 1 h and evaporated to dryness. The residue was dissolved in DMF (10 mL), Intermediate 5, pyrrolidine HCl (520 mg, 4.8 mmol) was added. Then BOP (2.2 g, 4.8 mmol) was added in portions under cooling in an ice bath for 16 h at room temperature. The disappearance of the starting hydroxy acid was monitored by LC/MS. The reaction mass was evaporated to dryness under 1 mmHg. Water (100 mL), EtOAc (50 mL), and a saturated solution of K₂CO₃ (to pH 10) were added. The layers were separated, and the aqueous one was subjected to extraction with EtOAc (2×50 mL). The combined organic layer was washed with water (50 mL), brine, dried with Na₂SO₄, and evaporated. The residue was purified by chromatography (60 mL of silica gel 63/100 μm, hexane/CHCl₃ 20:80→0:100, then CHCl₃/MeOH 100:0→90:10). The product-containing fraction were collected and concentrated to give the title compound (1.03 g, 63%). LC/MS data: 377.2 and 379.2 (M)⁺ (calculated for C₂₁H₂₉ClN₂O₂ 376.93). 1H NMR data (DMSO-d6), δ 7.51 (s, 1H, Ar—H); 7.42-7.49 (m, 2H, Ar—H), 5.75 (s, 1H, OH); 3.96 (d, 1H, J=13.7 Hz), 3.25-3.35 (m, ?H+H₂O); 2.78-2.90 (m, 2H); 2.53-2.60 (m, 2H); 2.43-2.52 (m, ?H+DMSO); 2.06-2.16 (m, 1H); 1.88-1.97 (m, 1H); 1.80-1.87 (m, 2H); 1.72-1.79 (m, 2H); 1.57-1.67 (m, 2H); 1.29-1.40 (m, 1H); 1.11 (d, 3H, J=5.8 Hz, CH₃).

Intermediate 6

(2R)-1+{2-chloro4-[3-(pyrroidin-1-ylcarbonyl)cyclobut-1-en-1-yl]benzyl}-2- methylpyrrolidine, trifluoroacetate

A solution of Example 4, 1-(3-Chloro-4-{[(2R)-2-methylpyrrolidin-1-yl]methyl}phenyl)-3-(pyrrolidin-1-ylcarbonyl)cyclobutanol (400 mg, 1.06 mmol) and TFA (1.64 ml, 21.2 mmol) in 4 ml of DCE was refluxed under argon for 6 h, then additional amount of TFA (1.64 ml, 21.2 mmol) was added and the mixture was refluxed for 24 h. The mixture was evaporated to dryness. According to LCMS data the reaction mixture contained up to 80% of the title compound (359, 360, 361 (M+H) (calculated for C₂₁H₂₇ClN₂O 358.92)). The resulting mixture was used for the next step without additional purification.

EXAMPLE 5 (2R)-1-{2-Chloro-4-[trans-3-(pyrrolidin-1-ylcarbonyl)cyclobutyl]benzyl}-2- methylpyrrolidine hydrochloride

To a solution of Intermediate 6, (2R)-1-{2-chloro-4-[3-(pyrrolidin-1-ylcarbonyl)cyclobut-1-en-1-yl]benzyl}-2-methylpyrrolidine, trifluoroacetate (1.06 mmol) in 5 mL of ethanol was added chlorotris(triphenylphosphine)rhodium(I) (100 mg, 0.106 mmol). The mixture was hydrogenated (40 psi H₂, at 50° C.) for 3 h. The reaction was monitored by LCMS. The mixture was evaporated to dryness, then 5 ml of 1 N HCl was added to the residue and the solution was extracted with ethyl acetate (2×5 ml), the organic layers were discarded. 10 N NaOH (1 mL) was added to the water layer and the water solution was extracted with ethyl acetate (3×5 mL). The organic layers were dried and evaporated in vacuo. The residue was purified by chromatography (SiO₂ 63/100 μm, 10 g, CHCl₃/hexane 80:20→100:0, CHCl₃/MeOH 100:0→90:10). The product containing fractions were collected and concentrated under reduced pressure. The residue was dissolved in 2 ml of ether and 0.1 mL of 4 N HCl/dioxane was added under stirring. The solvent was evaporated; the residue was dried in vacuum to afford the HCl salt of the title compound (80 mg, 20%) as a dark yellow amorphous solid. LCMS data: 361 and 363 (M+H)⁺ (calculated for C₂₁H₂₉ClN₂O 360.93). ¹H NMR data (CD₃OD): δ 7.63 (d, 1H, J=7.5 Hz), 7.50 (s, 1H), 7.39 (d, 1H, J=7.5 Hz), 4.75 (d, 1H, J=13.3 Hz), 4.31 (d, 1H, J=13.3 Hz), 3.63-3.77 (m, 2H), 3.34-3.52 (m, 7H), 2.64-2.75 (m, 2H), 2.33-2.49 (m, 3H), 1.72-2.20 (m, 7H), 1.51 (d, 3H, J=6.3 Hz).

EXAMPLE 6 (2R)-1-{2-Chloro-4-[cis-1-fluoro-3-(pyrrolidin-1-ylcarbonyl)cyclobutyl]benzyl}-2- methylpyrrolidine HCl

A solution of Example 4, 1-(3-Chloro-4-{[(2R)-2-methylpyrrolidin-1-yl]methyl}phenyl)-3-(pyrrolidin-1-ylcarbonyl)cyclobutanol (250 mg, 0.66 mmol) in 2 ml CH₂Cl₂ was added over 5 min to a solution of Deoxo-fluor (282 mg, 1.27 mmol) in CH₂Cl₂ (1 ml) in a flow of argon at −78 to −80° C. The reaction mixture was stirred at −78 to −80° C. for 1 h. The mixture was allowed to warm to 0° C. After 2 h, water (50 mL) was added followed by addition of 10 N NaOH, pH˜10. The layers were separated, and the aqueous one was subjected to extraction with CH₂Cl₂ (2×30 mL). The combined organic layer was washed wtth brine, dried with Na₂SO₄, and evaporated. The residue was purified by chromatography (10 mL of silica gel 63/100 μm, hexane/CH₂Cl₂ 20:80→0:100, then CH₂Cl₂/i-PrOH 100:0 →95:5). The product containing fractions were collected and concentrated. The residue was dissolved in ether (3 mL) and then 4 N HCl/dioxane (0.125 mL) was added, evaporated and dried in vacuo to give the HCl salt of the title compound (158 mg, 57%) as a yellow oil. LC/MS data: 379.2 and 381.2 (M+H)⁺ (calculated for C₂₁H₂₈ClFN₂ 378.92). 1H NMR data (DMSO-d6): δ 7.68-7.73 (m, 2H, Ar—H); 7.55-7.60 (m, 1H, Ar—H); 4.34 (d, 1H, J=13.4 Hz), 3.63-3.75 (m, 2H), 3.34-3.53 (m, 7H); 2.77-2.92 (m, 4H); 2.36-2.46 (m, 1H); 2.10-2.19 (m, 1H); 1.96-2.07 (m, 3H): 1.87-1.94 (m, 2H); 1.72-1.84 (m, 1H); 1.52 (d, 3H, J=6.3 Hz, CH₃).

Intermediate 7

3-(Morpholin-4-ylcarbonyl)cyclobutanone

CDl (8.1 g, 50 mmol) was added to a solution of 3-oxo-cyclobutanecarboxylic acid (5 g, 44 mmol) under vigorous stirring and cooling with an ice bath at 0° C. for 5 min. The reaction mixture was heated to 25° C., stirred at this temperature for 1 h, cooled to ° C., and morpholine (4.5 mL, 50 mmol) was added. The reaction mixture was heated to 25° C., stirred at this temperature for 3 h, and evaporated in vacuo. The residue was subjected to chromatography on SiO₂ (600 mL, 40-63 μm, CCl₄→CHCl₃→5% i-PrOH) to give compound 4 (6.5 g, 81%) as a colorless oil which solidified in the refrigerator. LC MS—data: M⁺ 184.1 and 185.1 (calculated for C₁₉H₁₃NO₄ 183.21) ¹H-NMR (400 MHz)·data (dmso-d6), δ 3.54-3.60 (nm, 4H), 3.43-3.52 (m, 5H), 3.16-3.32 (m, 4H).

EXAMPLE 7 [3-Hydroxy-3-(4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-morpholin-4-yl- methanone

To a stirring solution of 1-(4-bromo-benzyl-pyrrolidine (1.6 g, 6.5 mmol) in THF (20 mL) at −78° C. (acetone/dry ice bath) was added slowly down the side of the flask a solution of nBuLi (2.6 mL, 6.5 mmol, 2.5 M THF). After 15 minutes a solution of Intermediate 7, 3-(morpholin-4l-ycarbonyl)cyclobutanone (1.0 g, 5.4 mmol, in 7 mL of THF) precooled to −78° C. was added slowy. After 30 minutes the reaction was quenched cold with 1 N HCl (20 mL). This mixture was diluted with EtOAc and then the layers were separated and the organic layer was discarded. The aqueous layer was basified with 1 N NaOH and extracted with CHCl₃/iPrOH (3:1). The organic layer was dried over MgSO₄, filtered and concentrated to give a yellow oil. This material was purified by flash column chromatography using a 40 g ISCO™ column, eluting with a gradient of 3%, 5%, 10%, 20%, 30% MeOH/CHCL₃ with 0.1% NH₄OH. The product containing fraction were collected and concentrated under reduced pressure to give the title compound (379 mg, 20% yield): R_(f)=0.3 (30% MeOH/CH₂Cl₂); LRMS m/z Calcd for C20 H28 N2 O3, 344.4, found, 345 (M+1) APCl: 400 MHz ¹H NMR (CDCl₃) δ 7.42 (d, J=8.3 Hz, 2H), 7.29 (d, J=8.3 Hz, 2H), 4.58 (brs, 1H), 3.62-3.55 (m, 8H), 3.34-3.32 (m, 2H), 2.87 (dddd, J=8.3, 8.3, 8.3, 8.3 Hz, 1H), 2.77-2.71 (m, 2H), 2.64-2.59 (m, 2H), 2.48-2.45 (m, 4H), 1.75-1.70 (m, 4H); 100 MHz ¹³C NMR (CDCl₃) δ 173.8, 144.4, 137.8, 129.3, 125.3, 72.9, 67.0, 66.9, 60.3, 54.2, 46.0, 42.6, 40.9, 28.1, 23.5.

Intermediate 8

1-(4-bromo-2-fluorobenzyl)pyrrolidine

A 4-L RB flask, equipped a magnetic stirring bar was charged with pyrrolidine (363 g, 426 mL, 5.1 mol) and acetonitrile (2750 mL). The mixture was cooled with an ice bath to 10° C., then solid 4-bromo-2-fluorobenzylbromide (MATRIX, Cat. #: 1707, 375 g, 1.4 mol) was added in 6 portions, keeping temperature below 20° C. The mixture was stirred at RT for 4 h. The solvent was removed under vacuum. Then 2 L of sat. Na₂CO₃ aq. and 500 mL of water was added, and the mixture was extracted with DCM (3×700 mL). The extract was dried over Na₂SO₄ and evaporated. The resulting light yellow oil was distilled under vacuum (˜1 mm, bp. 125° C.) to give 324.5 g (90%) of the product as a colorless oil. LCMS (M+H): 258.5.

¹H NMR (300 MHz, CDCl₃); δ 7.19-7.31 (m, 3H), 3.63 (m, 2H), 2.53 (m, 4H) 1.78 (m, 4H).

Intermediate 9

3-[3-fluoro-4-(pyrrolidin-1-ylmethyl)phenyl]-3-hydroxycyclobutanecarboxylic acid

A 2-L 3-neck RB flask, equipped a mechanical stirrer, addition funnel, thermometer and nitrogen gas inlet was charged with Intermediate 8, 1-(4-bromo-2-fluorobenzyl)pyrrolidine (69.86 g, 0.27 mol) and 700 mL of anhydrous THF. The system was flushed with nitrogen and cooled to −85° C. with liquid nitrogen with an ether/MeOH (1:1) bath. Then n-BuLi (10 M in hexane, 30 mL, 0.298 mol) was added dropwise through an addition funnel at T<˜80° C. The mixture was stirred at this temperature for an additional 15 min, then a solution of 3-oxocyclobutanecarboxylic acid (dried under vacuum for 2 days, 15.4 g, 0.135 mol) in 300 mL of anhydrous THF was added dropwise through an addition funnel keeping T<˜80° C. The mixture was allowed to warm to RT and evaporated. The residue was mixed with 500 mL of water and washed with ether (2×300 mL). Then the aqueous solution was acidified to pH 1 with conc. HCl and washed with ether (2×300 mL). Then the aqueous solution was neutralized to pH 6-5 with NaOH and coevaporated three times with iPrOH (300 mL each time). Then the mixture was coevaporated with THF (200 mL) and dried to give gummy residue containing the product with inorganic salts LCMS (M+H): 294.4

This material was used directly for the next step.

EXAMPLE 8 N-{2-fluoro-4-[1-hydroxy-3-(pyrrolidin-1-ylcarbonyl)cyclobutyl]benzyl}- pyrrolidine

A 2-L 3-neck RB flask, equipped a mechanical stirrer, addition funnel and nitrogen gas inlet was charged with Intermediate 9, 3-[3-fluoro-4-(pyrrolidin-1-ylmethyl)phenyl]-3-hydroxycyclobutanecarboxylic acid (0.135 mol, crude material fromn the above intermediate), 500 mL of anhydrous THF and DIEA (34.8 g, 0.27 mol). The initially insoluble mixture was stirred for 1.5 h until a uniform suspension formed. Then 2,4,6-tripropyl-1,3,5,2,4,6-trioxatriphosphonrinane-2,4,6-trioxide (50% solution in EtOAc, 104.5 mL, 0.164 mol) was added and stirred for 5 min. (NOTE: exotherm was observed, reached ˜45-50° C.). Then pyrrolidine (28.2 mL, 24.0 g, 0.337 mol) was added. (NOTE: more exotherm was observed, reached ˜70-80° C. The mixture was stirring at RT for 12 h, then evaporated. The residue was mixed vith 500 mL of sat. Na₂CO₃ and 200 mL of water. The mixture was extracted with DCM (5×300 mL), the extract was dried over Na₂SO₄, evaporated and dried to give 33.4 g (71% for two steps) of pure title compound (LCMS (M+H): 347.1. ¹H NMR (300 MHz, CDCL₃): δ 7.37 (t, J=7.37 Hz, 1H), 7.17-7.26 (m, 2H), 3.68 (s, 2H) 3.53 (t, J=6.78 Hz, 2H), 3.44 (t, J=6.58 Hz, 2H), 3.03-3.14 (m, 1H), 2.79-2.87 (m, 2H), 2.5-2.6 (m, 6H), 1.87-2.00 (m, 4H), 1.75-1.80 (m, 4H).

EXAMPLE 9 N-{2-fluoro-4-[1-fluoro-3-(pyrrolidin-1-ylcarbonyl)cyclobutyl]benzyl}-pyrrolidine hydrochloride

A 2-L 3-neck RB flask, equipped a magnetic stirring bar, thermometer, addition funnel and nitrogen gas inlet was charged with Example 8, N-{2-fluoro-4-[1-hydroxy-3-(pyrrolidin-1-ylcarbonyl)cyclobutyl]benzyl}-pyrrolidine (43.0 g, 0.124 mol) and 1 L of anhydrous DCM under nitrogen. The mixture was cooled to −75° C. with a dry ice/acetone bath, then Deoxo-Fluor (Aldrich, 33.0 g, 27.5 mL, 0.149 mol) was added dropwilse. The mixture w,4as warmed to 0° C. and stirred for 30 mtn at this temperature. Then the mixture was quenched with 350 mL of sat. Na₂CO₃, and extracted with DCM (3×300 mL). The extract ewas dried over Na₂SO₄ and evaporated. The resulting crude oil was purified by cournn (silica gel, ether 60%, hexane 30%, MeOH 5%, Et₃N 5%, Rf=0.37 in ether 60%, hexane 30%, MeOH 5%, NH₄OH 5%) to give 29.0 g (67%) of the title compound. ¹H NMR (300 MHz, CDCl₃): δ 7.39 (t, J=7.64 Hz, 1H), 7.21 (d, J=7.92 Hz, 1H), 7.14 (d, J=10.9 Hz, 1H), 3.67 (s, 2H), 3.40-3.61 (m, 5H), 2.66-3.00 (m, 4H), 2.50-2.55 (m, 4H), 1.80-2.00 (m, 4H), 1.75-1.80 (m, 4H).

The free base of the product (29.0 g) was dissolved in 500 mL of ether, then 83 mL of 2M HCl/ether was added dropwise, stirred for 30 min, filtered and dried under vacuum to give 32.5 g of hydrochloride salt (NMR: contains approximately 4.5% of cis isomer). Then this material was dissolved in 200 mL of water, basified with NaOH to pH 10, extracted with DCM (3×300 mL) evaporated and purified by column again to give 25.0 g of free base product (NMR: contains approximately 3.5% of cis isomer). Then this 25 g of free base was converted to HCl salt as above. The HCl salt was recrystallized by dissolving in 250 mL of EtOAc/50 mL MeOH at 60° C., cooling dovn to RT, and stirring for 2 h. The precipitate was collected by filtration and dried to give 8.0 g of first crop (NMR: contains approximately 3% of cis isomer). The remaining mother liquor was concentrated under vacuum to 100 mL, then 100 mL of EtOAc vwas added and stirred for 30 min. The precipitate was filtered, combined with the first crop and dried under vacuum for 2 days to give 18.86 g of HCl salt (NMR:—contains approximately 3% of cis isomer). (LCMS (M+H): 349.5. ¹H NMR (300 MHz, CDCl₃): δ 12.66 (br. s, 1H), 7.97 (t, J=7.81 Hz, 1H), 7.41 (d, J=8.1 Hz, 1H), 7.32 (d, J=10.9 Hz, 1H), 4.29 (d, J=5.25 Hz, 2H), 3.55-3.70 (m, 3H), 3.45-3.55 (m, 4H), 2.70-3.05 (m, 6H), 2.20-2.30 (m, 2H), 2.03-2.13 (m, 2H), 1.90-2.00 (m, 4H).

Intermediate 10

1-(4-Bromo-2-chloro-5-fluorobenzoyl)pyrrolidine

To a stirring solution of 4-bromo-2-chloro-5-fluorobenzoic acid (50 g, 0.25 mol) in 200 mL of EtOAc at 0° C. (ice/water bath) was added triethyl amine (237 mL, 0.50 mol), pyrrolidine (41.2 mL, 0.5 mol), followed by 2,4,6-tripropyl-1,3,5,2,4,6-trioxatriphosphorinane-2,4,6-trioxide (CAS # 68957-94-8) (237 mL, 0.37 mol, 50 wt %, EtOAc). After 1 hr, the reaction was quenched with a saturated solution of NaHCO₃, and extracted with EtOAc, and CH₂Cl₂. The combined organic layers were dried over MgSO₄, filtered and concentrated. Purification of this material was accomplished by flash column chromatography using a Biotage™ 75 L column, eluting with a gradient of 2%-50% EtOAc/hexanes. The product containing fractions were collected and concentrated to give the title compound (52 g, 68% yield) as a white solid: R₁ =0.23 (40% EtOAc/hexanes): LRMS m/z Calcd for C₁₁H₁₀BrClFNO, 306.6, found, 306, 308, 310 (M+1) APCl; ¹H NMR (300 MHz, CDCl₃): δ 7.53 (d, J=6.2 Hz, 1H), 7.02 (d J=7.9 Hz, 1H), 3.54 (apt t, J=6.6 Hz, 2H), 3.13 (apt t, J=6.6 Hz, 2H), 1.92-1.83 (m, 4H); 100 MHz ¹³C NMR (CDCl₃) δ 164.5, 158.1 (d, J_(C−F) =249.5 Hz), 138.2, 134.3, 126.0, 115.5 (d, J_(C−F) =25.5 Hz), 110.3 (d, J_(C−F) =22.5 Hz), 47.0, 45.8, 26.0, 24.6.

Intermediate 11

1-(4-Bromo-2-chloro-5-fluorobenzyl)pyrrolidine

To Intermediate 10, 1-(4-bromo-2-chloro-5-fluorobenzoyl)pyrrolidine (48.0 g, 156.5 mmol) in THF (200 mL) at rt was slowly added a solution of BH₃·THF comnplex (400 mL, 400 mmol, 1M THF). The resulting reaction was heated to 65° C. (oil bath) for 16 hr, and then the reaction was cooled to rt and slowly quenched with MeOH (dropwise addition). The reaction mixture was heated to reflux for 2 hr, cooled to rt, and concentrated under reduced pressure. This material was taken up in EtOAc and further quenched slowly with 6N HCl, then neutralized with aqueous NaOH (15%). The layers were separated and the aqueous layer was back extracted with EtOAc. The combined organic layers were dried over MgSO₄, filtered and concentrated under reduced pressure. Purification of this material was accomplished by flash column chromatography using a Biotage™ 75 L column, eluting with a gradient of 5%, 10% MeOH/CH₂Cl₂. The product containing fractions were collected and concentrated to give the title compound (43 g, 94% yield) as a light yellow oil: R₁=0.6 (10% MeOH/CH₂Cl₂); LRMS m/z Calcd for C₁₁H₁₂BrClFN, 292.6, found, 292, 294, 296 (M+1) APCl; ¹H NMR (300 MHz, CDCl₃): δ 7.51 (d, J=6.6 Hz, 1H), 7.33 (d, J=9.5 Hz, 1H), 3.66 (s, 2H), 2.59-2.55 (m, 4H), 1.82-1.79 (m, 4H): 100 MHz ¹³C NMR (CDCl₃) δ 158.3 (d, J_(C-F)=247.2 Hz), 139.3, 133.3, 128.9, 117.8 (d, J_(C−F)=24.9 Hz), 107.3 (d, J_(C−F)=22.6 Hz), 56.7, 54.4, 23.9

EXAMPLE 10 3-(5-Chloro-2-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy- cyclobutanecarboxylic Acid isobutyl-amide

To Intermediate 11, 1-(4-Bromo-2-chloro-5-fluorobenzyl)pyrrolidine (4.0 g 13.7 mmol) in THF (34 mL) at −78° C. (acetone/dry ice bath) was added a solution of nBuLi (5.5 mL, 13.7 mmol, 2.5 M THF). After 15 min, a precooled (−78° C.) solution of 3-oxocyclobutanecarboxylic acid (0.78 g, 6.8 mmol, in 5 mL of THF) was added via cannula. The reaction was allowed to slowly warm to rt overnight. After approximately 16 h, isobutylamine (1.4 mL, 13.7 mmol) was added, followed by 2,4,6-tripropyl-1,3,5,2,4,6-trioxatriphosphorinane-2,4,6-trioxide (50% solution in EtOAc, 6.6 mL, 10.2 mmol). After 1 hr the reaction was diluted vwth EtOAc and then quenched with 1N NaOH. The layers vere separated and the aqueous layer was back extracted with EtOAc. The combined organic layers were dried over MgSO₄, filtered and concentrated under reduced pressure. Purification of this material was accomnplished by flash column chromatography using a 120 g ISCO™column, eluting with 5% MeOH/CH₂Cl₂ with 0.1% NH₄OH. The product containing fractions were collected and concentrated to give the title compound (400 mg, 15% yield) as a yellow foam; R_(t)=0.23 (10% MeOH/CH₂Cl₂), LRMS m/z Calcd for C₂₀H₂₈Cl FN₂O₂, 382.9, found, 383, 385 (M+H) APCI; ¹H NMR (300 MHz, CDCl₃) δ 7.37 (d, J=7.0 Hz, 1H), 7.20 (d, J=11.6 Hz, 1H), 6.25-6.22 (bm, 2H), 3.68 (s, 2H), 3.09-2.84 (m, 5H), 2.57 (apt bs, 4H), 2.46-2.43 (m, 2H), 1.79-1.70 (m, 5H); 0.88 (d, J=6.6 Hz, 6H), 100 MHz ¹³C NMR (CDCL₃) δ 177.5, 159.5 (d, J_(C−F)=247.2), 138.3, 132.1, 128.2: 117.9 (d, J_(C−F)=24.7 Hz), 73.2, 56.5, 54.3, 47.4, 40.1, 34.6, 28.7, 23.8, 20.3.

EXAMPLE 11 3-(5-Chloro-2-fluoro-4-pyrolidin-1-ylmethyl-phenyl)-3-fluoro- cyclobutanecarboxylic acid isobutyl-amide

To 3 mL of CH₂Cl₂ at −78° C. (acetone/dry ice bath) was added BAST (251 uL, 1.4 mmol), followed by a solution of Example 10, 3-(5-chloro-2-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid isobutyl-amide (350 mg, 0.91 mmol in 2 mL of CH₂Cl₂). After 1 hr the reaction was quenched with a saturated solution of sodium bicarbonate and then diluted with EtOAc. The layers were separated and the aqueous layer was back extracted with EtOAc. The combined organic layers were dried over MgSO₄, filtered and concentrated under reduced pressure. Purification of this material was accomplished by flash chromatography using a 40 g ISCO™column, eluting with 5% MeOH/CH₂Cl₂. The product containing fractions were collected and concentrated to give the title compound as a mixture of isomers (223 mg, 63% yield) as a yellow oil: R_(t)=0.45 (10% MeOH/CH₂Cl₂); The mono HCl salt was made by dissolving the title compound in EtOAc and adding a 2N HCl ether solution (1.2 eq). The resulting solid was stirred 2 hr and then filtered and dried under reduced pressure to give the HCl salt of the title compound as a yellow solid: LRMS m/z Calcd for C₂₀H₂₇ClF₂N₂O, 384.9, found, 386, 388 (M+H) APCl: ¹H NMR mixture of isomers, diagnostic peaks major isomer (300 MHz, CD₃OD); δ 7.62 (dd, J=7.0, 1.6 Hz, 1H), 7.54 (d, J=11.2 Hz, 1H), 4.47 (s, 2H), 3.59-3.47 (m, 2H), 3.43 (apt pent, J=7.3 Hz, 1H), 3.31-3.02 (m) under MeOH, 3.01-2.77 (m, 6H), 2.24-2.20 (m, 2H), 2.06-2.00 (m, 2H), 1.78-1.71 (m, 1H), 0.89 (d, J=7.0 Hz, 6H).

EXAMPLE 12 3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic Acid Ethylamide

n-Butyllithium (2.5 M/hexanes, 251 mL, 0.628 mol) was added dropwise over 30 min to a −78° C. solution of 1-(4-bromo-2-fluorobenzyl)pyrrolidine (162.0 g, 0.63 mol) in THF (1.8 L). After stirring at −78° C. for 2 h, a −78° C. solution of 3-oxocyclobutanecarboxylic acid (35.8 g, 0.31 mol) in THF (400 mL) was cannulated over 25 min into the reaction mixture. The resulting dark orange solution was slowly warmed to room temperature over 16 h. LC/MS of the mixture showed the intermediate acid 294.2 (M+H). Ethylamine (2M in THF, 315 mL, 0.630 mol) and T3P (50% wt in EtOAc, 224 mL, 0.376 mol) were added with 200 mL of rinse THF. After stirring for 1 h at room temperature, sat. NaHCO₃ (1000 mL) was added followed by water (˜500 mL). The phases were separated and the aqueous phase was extracted with EtOAc (2×500 mL). The combined organics were washed with brine and dried over MgSO₄. Concentration yielded 161.8 g of orange oil that was split into 2 portions and purified by SiO₂ flash chromatography (4″×5.5″ columns packed with EtOAc). Each column was flushed with 3L EtOAc to remove higher Rf material and then the bulk of the desired product was obtained by elution with 3L 25% MeOH/EtOAc. Concentration of the product containing fractions from both columns afforded 48.8 g (49% yield) of 3-(3-f;uoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid ethylamide as a thick light orange oil which slowly crystallized to a waxy solid upon evacuation: ¹H NMR (CDCl₃) δ 7.35 (t, J=7.9 Hz, 1H), 7.20-7.14 (m, 2H), 5.67 (br s, 1H), 5.57 (br s, 1H), 3.66 (d, J=1.3 Hz, 2H) 3.37-3.30 (m 2H), 2.84-2.70 (m, 3H), 2.53-2.44 (m, 6H), 1.83-1.70 (m overlapping water, 4H), 1.16 (t, J=7.3 Hz, 3H), LRMS m/z Calcd for C₁₅H₂₅FN₂O₂, 320.4, found, 321.3(M+H) APCl.

EXAMPLE 13 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic Acid ethylamide

To Example 12, 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid ethylamide (17 g, 53.1 mmol) in 200 mL of DCE at rt was added TFA (80.7 mL, 1.1 mol) and then the reaction was heated to 80° C. (oil bath). After 15 h the reaction was concentrated to approximately 45 g and used without further purification. The crude TFA salt from the above reaction was diluted with EtOH (500 mL), placed in a Parr bottle, purged with N₂, and then 10% Pd/carbon (2.5 g, 14 wt %) was added. The resulting reaction mixture was hydrogenated with H₂ (45 psi) at rt. After 1.5 h the reaction was purged with N₂, then filtered through Celite™ and concentrated under reduced pressure. The resulting oil was diluted with EtOAc and then slowly quenched with a saturated solution of sodium bicarbonate. The layers were separated and the aqueous layer was back extracted with EtOAc. The combined organic layers were dried over MgSO₄, filtered and concentrated under reduced pressure. Purification of this material was accomplished by re-crystallization. Crude title compound was taken up in a minimal amount of warm EtOAc and allowed to cool to approximately 0° C. (refrigerator). The solid was filtered, dried under reduced pressure to give the title compound (4 g, 24% yield) as a white solid: R_(t)=0.21 (10% MeOH/CH₂Cl₂); LRMS m/z Calcd for C₁₈H₂₅FN₂O, 304,4, found, 305.3, ¹H NMR (300 MHz, CDCl₃): δ 7.30 (apt t J=7.9 Hz, 1H), 6.98 (dd, J=7.9, 1.6 Hz, 1H), 6.92 (dd, J=12.0, 1.6 Hz, 1H), 5.36 t,(bs 1H), 3.67 (s, 2H), 3.42-3.26 (m, 3H), 2.94-2.85 (m, 1H), 2.57-2.50 (m, 6H) 2.42-2.33 (m, 2H), 1.82-1.74 (m, 4H), 1.14 (t, J=7.5 Hz, 3H); structure confirmed by x-ray crystallography and was determined to be cis.

EXAMPLE 14 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic Acid ethyl-methyl-amide

n-Butyllithium (2.5M/hexanes, 140 mL, 0.350 mol) was added down the reaction flask walls over 25 min to a −78° C. solution of Intermediate 8, 1-(4-bromo-2-fluorobenzyl)pyrrolidine (90.0 g, 0.349 mol) in THF (1 L). After stirring at −78° C. for 2.5 h, a −78° C. solution of 3-oxocyclobutanecarboxylic acid (19.9 g, 174.5 mmol) in THF (200 mL) was cannulated over 15 min. into the reaction mixture. The resulting dark orange solution was slowly warmed to room temperature over 16 h. LC/MS of the mixture showed the intermediate acid 294.2 (M+H) Ethylmethylamine (30 mL, 0.349 mol) and T3P (50% wt in EtOAc, 125 mL, 0.210 mol) were added with 200 mL of rinse THF. After stirring for 1.5 h at room temperature, sat. NaHCO₃ (500 mL) was added followed by water (500 mL). The phases were separated and the aqueous phase was extracted with EtOAc (700 mL). The combined organics were washed with brine and dried over MgSO₄. Concentration yielded 89.0 g of orange oil that was purified by SiO₂ flash chromatography (4⁰×7⁰ column packed with EtOAc). The column was flushed with 4L EtOAc to remove higher Rf material and then the bulk of the desired product was obtained by elution with 4 L 25% MeOH/EtOAc. Concentration of the product containing fractions afforded 35.15 g (60%) of the title compound, 3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid ethyl-methyl-amide as a thick light orange oil which slowly crystallized to a waxy solid upon evacuation: NMR (CDCl₃) ˜1:1 mixture of rotamers, δ 7.35 (t, J=7.7 Hz, 1H), 7.23- 7.15 (m, 2H), 5.08 and 4.84 (broad singlet, 1H total), 3.65 (s, 2H), 3.45 and 3.30 (quartets, J=7.2 Hz, 2H total), 3.21-3.10 (m, 1H), 2.97 and 2.95 (singlet, 3H total), 2.84-2.77 (m, 2H), 2.57-2.52 (m, 6H), 1.79-1.72 (m, 4H), 1.18-1.04 (m, 3H)

Intermediate 12

N-ethyl-3-(3-fluoro-4-(pyrrolidin-1-ylmethyl)phenyl)-N-methylayclobut-2- enecarboxamide trifluoroacetate salt

3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid ethyl-methyl-amide (Example 14, 35.15 g, 105.1 mmol) was dissolved in a mixture of 1,2-dichloroethane (1L) and trifluoroacetic acid (150 mL) and refluxed for 16 h. The resulting dark brown solution was cooled and concentrated to yield a brown oil (94.46) of crude title compound, N-ethyl-3-(3-fluoro-4-(pyrrolidin-1-ylmethyl)phenyl)-N-methylcyclobut-2-enecarboxamide trifluoroacetate salt, with residual TFA, which was used in the next step without purification.

EXAMPLE 15 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethyl- methyl-amid

A hydrogenation vessel was rinsed with ethanol, purged with nitrogen and charged with 50 mL ethanol, 10% palladium on carbon (10 g) and a solution of crude Intermediate 12, N-ethyl-3-(3-fluoro-4-(pyrrolidin-1-ylmethyl)phenyl)-N-methylcyclobut-2-enecarboxamide trifluoroacetate salt (182.0 g) in ethanol (1.5L). This mixture was then shaken under hydrogen (˜45 psi) at room temperature for 1.5 h, filtered through a 2″ pad of diatomaceous earth and rinsed with ethanol (500 mL). The filtrate was concentrated to give an orange oil which was dissolved in EtOAc (500 mL) and washed with a solution of K₂CO₃ (60 g) in water (400 mL) and then brine (200 mL), dried over MgSO₄ and concentrated to afford 66.26 g of an orange oil. This material was purified by flash chromatography on a 4″×5.5″″ silica gel columnn (CH₂Cl₂ packed), flushing first with 2.5 L CH₂Cl₂ and then eluting with 3L 5% MeOH/CH₂Cl₂. The clean product fractions were concentrated to afford 41.75 g (58%) of >95% pure title compound. Concentration of the less pure fractions yielded another 9.98 g of ˜85-90% pure material: R_(f)=0.17 (20% MeOH/EtOAc); ¹H NMR (CDCl₃) ˜1:1 mixture of rotamers, δ 7.28-7.24 (m partially obscured by CHCl₃, 1H), 6.98-6.94 (nm, 1H), 6.92-6.88 (m, 1H), 3.62 (d, J=0.8 Hz, 2H), 3.42-3.14 (overlapping multiplets, 4H), 2.92 and 2.89 (singlets, 3H total), 2.58-2.35 (m, 8H), 1.80-1.70 (m, 4H), 1.14 and 1.08 (triplets, J=7.2 Hz, 3H total).

The cleaner material (41.75 g, 131.11 mmol) was dissolved in EtOAc (1L) and 2N HCl/diethylether (80 mL, 160 mmol) was added over ˜1 min with vigorous stirring. After 30 min, the light orange tinged precipitate was collected, rinsed with EtOAc and dried under nitrogen purge to yield the corresponding HCl salt (36.15 g). This material was combined with other lots (39.72 g total weight), and dissolved in a mixture of MeOH (30 mL) and EtOAc (50 mL) with gentle heating. Next, EtOAc (550 mL) was added dropwise over ˜15 min to the stirring mixture. After stirring an addiional 15 min at room temperature, the solids were filtered, rinsed with 200 mL EtOAc and dried under nitrogen to yield 32.98 g of the hydrochloride salt of the title comnpound as a white crystalline solid: mp 196-196.5° C.; ¹H NMR (CDCl₃) mixture of rotamers, δ 12.69 (br s, 1H), 7.79 (t, J=7.9 Hz, 1H), 7.07-7.00 (m, 2H), 4.20 (d, J=5.4 Hz, 2H), 3.64-3.57 (m, 2H) 3.47-3.53 (m, 2H), 3.30-3.16 (m, 2H) 2.91 and 2.88 (singlets, 3H total), 2.85-2.79 (m, 2H), 2.60-2.50 (m, 2H), 2.46-2.34 (m, 2H), 2.26-2.14 (m, 2H), 2.06-1.95 (m, 2H), 1.14 and 1.07 (triplets, J=7.1 Hz, 3H total); ¹³C NMR (CDCl₃) δ (mixture of rotamers) 173.29, 162.70, 160.24, 150.57, 150.49, 133.62, 133.59, 123.99, 123.97, 114.44, 114.30, 113.98, 113.77, 52.62, 49.92, 49.90, 44.07, 42.65, 35.33, 34.25, 33.45, 32.77, 32.40, 23.21, 14.07, 12.44; LRMS m/z Calcd for C₁₉H₂₇FN₂O, 318.4, found, 319.4 (M+H) APCl; Anal. Calculated for C₁₉H₂₇FN₂O HCl: C64.30, H 7.95, N 7.89. Found C 64.36, H 8.02, N 7.97.

EXAMPLE 16 3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethylamide

A solution of Example 12, 3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid ethylamide (48.7 g, 152.0 mmol) in CH₂Cl₂ (450 mL) was added over 50 min down the reaction flask walls to a −78° C. solution of bis(2-methoxyethyl)aminosulfur trifluoride (42.0 mL, 227.8 mnmol) in CH₂Cl₂ (375 mL). After stirring for 2.5 h at −78° C., the cooling bath was removed and the mixture was stirred for 16 h at room temperature. With stirring, aq. NaHCO₃ was carefully added in portions until all foaming subsided. Solid K₂CO₃ was then added to ensure that the pH was >8. The phases were separated and the aqueous phase was extracted with two additional 100 mL portions of CH₄Cl₂. The organic phases were combined, dried over MgSO₄ and concentrated to yield a dark orange-brown oil (50.2 g). This crude material was concentrated onto 100 g silica gel and then purified by flash chromatography on a 4⁰×6⁰ silica gel column packed with EtOAc. The column was eluted with 3L each EtOAc and 10% MeOH/EtOAc. The cleanest fractions were concentrated to afford 20.82 g of the title compound as an orange tinged solid which GC/MS showed to be ˜94% pure with ˜6% of the corresponding trans isomer. Re-purification of this lot and the less pure fractions was achieved by either repeating the MeOH/EtOAc column chromatography or by chromatography on a ChiralcelOD column (10 cm×50 cm) using 93:7 heptane:isopropyl alcohol with a flow rate of 460 mL/min. These re-chromatographed materials were then triturated with 10% ethyl ether/hexanes (˜8 mL/gram) to yield 28.21 g (58%) of 95+% pure title compound: R_(f)=0.23 (20% MeOH/EtCOAc); ¹H NRM (CDCl₃) δ 7.38 (t, J=7.7 Hz, 1H), 7.20 (d, J=8.3 Hz, 1H), 7.13 (dd, J=10.8, 1.6 Hz, 1H), 5.43 (br s, 1H), 3.66 (s, 2H), 3.33-3.18 (m, 3H), 2.92-2.78 (m, 2H), 2.76-2.64 (m, 2H), 2.58-2.48 (m, 4H), 1.81-1.70 (m, 4H), 1.13 (t, J=7.3 Hz, 3H).

The hydrochloride salt of the title compound was prepared by addition of 53 mL 2N HCl/ethyl ether to a stirring solution of freebase in 650 mL EtOAc. After stirring for ˜2h, the white precipitate was collected, wvashed with EtOAc and dried under a stream of nitrogen: mp 196.5-197.5° C.; ¹H NMR (MeOH-d₄) δ 7.60 (t J=7.7 Hz, 1H), 7.46-7.40 (m, 2H), 4.46 (s, 2H), 3.56-3.50 (m, 2H), 3.37 (p, J=8.5 Hz, 1H), 3.24-3.17 (m, 4H), 2.86-2.67 (m, 4H), 2.22-2.10 (m, 2H), 2.08-1.95 (m, 2H), 1.10 (t, J=7.3 Hz, 3H) ¹³C NMR (CDCl₃) δ 173.6, 161.3 (d, J_(C−F)=248.0 Hz), 147.1 (dd, J_(C−F)=24.0, 7.7 Hz), 134.0 (d, J_(C−F)=2.3 Hz), 121.9 (dd, J_(C−F)=8.3, 2.7 Hz), 116.4 (d, J_(C−F)=13.2 Hz), 112.6 (dd, J_(C−F)=24.1, 8.8 Hz), 96.7 (d, J_(C−F)=197.3 Hz), 52.83, 49.9 (d, J_(C−F)=3.0 Hz), 38.8 (d, J_(CF)=24.8 Hz), 34.8, 32.9, 23.3, 15.0, Anal. Calculated for C₁₈H₂₄F₂N₂O.HCl: C 60.25, H 7.02, N 7.81. Found C 60.15, H 7.32, N 7.60.

Intermediate 13

1-(4-bromo-3,5-difluorobenzyl)pyrrolidine

3,5-Difluorobenzaldehyde (2.0 mL, 18.24 mmol), pyrrolidine (1.8 mL, 21.56 mmol), and sodium triacetoxyborohydride (5.8 g, 27.4 mmol) were stirred in THF (50 mL) for 16 h at room temperature. Saturated aqueous NaHCO₃ (30 mL) was added and after stirring for 30 min, EtOAc (50 mL) was added. The organic phase was separated and washed with brine, dried over MgSO₄ and concentrated to afford 2.65 g (74%) of 1-(3,5-difluorobenzyl)pyrrolidine as a slightly cloudy oil: ¹H NMR (CDCl₃) δ 6.88-6.83 (m, 1H), 6.68-6.63 (m, 2H), 3.59 (s, 2H), 2.53-2.48 (m, 4H), 1.80-1.77 (m, 4H).

Intermediate 14

3-(2,6-difluoro-4-(pyrrolidin-1-ylmethyl)phenyl)-3-hydroxy-N-methylcyclobutanecarboxamide

2,2,6,6-Tetramethylpiperidine (1.86 mL, 11.0 mmol) was added to a −78° C. solution of n-butyllithium (2.5 M in hexanes, 4.4 mL, 11.0 mmol) in hexanes (12 mL) and THF (25 mL). The resulting mixture was stirred for 10 min and then intermediate 13, 1-3,5-difluorobenzyl)pyrrolidine (2.17 g, 11.0 mmol) in 3 mL THF was added down the flask walls over 1 min. After stirring for 2.5 h, a −78° C. solution of 3-oxocyclobutanecarboxylic acid (0.63 g, 5.5 mmol) in THF (10 mL) was cannulated into the reaction mixture. This mixture was allowed to slowly warm to room temperature and was stirred for 16 h. Methylamine (2.0 M in THF, 5.5 mL, 11.0 mmol) and T3P 50% wt in EtOAc, 3.9 mL, 6.55 mmol) were then added. After 2 h stirring, saturated aqueous NaHCO₃ was added and the mixture was extracted into EtOAc, dried over MgSO₄ and concentrated to yield a tan oil. Silica gel flash chromatography using first 3% then 15% MeOH/CH₂Cl₂ afforded 99 mg (5.5%) of the title compound, 3-(2,6-difluoro-4-(pyrrolidin-1-ylmethyl)phenyl-3-hydroxy-N-methylcyclobutanecarboxamide as a waxy white solid: R_(f)=0.036 (CH₂Cl₂); ¹H NMR (CDCl₃) δ 6.84-6.78 (m, 2H), 6.25-6.20 (br m, 1H), 3.52 (s, 2H), 3.01-2.95 (m, 2H), 2.90-2.84 (m, 1H), 2.79 (d, J=5.0 Hz, 3H), 2.58-2.54 (m, 2H), 2.48-2.44 (n, 4H), 1.77-1.73 (m, 4H); ¹³C NMR (CDCl₃) δ 222.6, 178.5, 161.2 (d, J_(C−F)=240.5 Hz), 142.2, 111.9 (dd, J_(C−F)=25.6, 6.8 Hz), 73.0, 59.7, 54 2, 40.8, 37.0, 26.8, 23.7.

EXAMPLE 17 3-(2,6-Difluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid methylamide

Bis(2-methoxyethyl)aminosulfur trifluoride (0.070 mL, 0.380 mmol) was added to a 0° C. solution of intermediate 14, 3-(2,6-difluoro-4-(pyrrolidin-1-ylmethyl)phenyl)-3-hydroxy-N-methylcyclobutanecarboxamide (0.099 g, 0.305 mmol) in CH₂Cl₂ (2 mL) and the resulting mixture was allowed to warm to room temperature and stir for 18 h. The reaction was poured into saturated aqueous NaHCO₃ and extracted with CH₂Cl₂ (2×15 mL), dried over MgSO₄ and concentrated to yield 92 mg of light yellow oil: R_(f)=0.21 (20% MeOH/EtOAc). Silica gel flash chromatography using EtOAc and then 5% and 10% MeOH/EtOAc for elution afforded 66 mg (67% yield) of the title compound: LRMS m/z Calcd for C₁₇H₂₁F₃N₂O, 326.4, found, 327.4 (M+H), 307.4 M+H−HF) APCl: ¹H NMR (CDCl₃) δ 6.87 (d, J=8.7 Hz, 2H), 5.42 (br s, 1H), 3.56 (m, 2H), 3.32 (P J=8.5 Hz, 1H), 3.06-2.78 (m, 7H), 2.50 (br s, 4H), 1.79 (br s, 4H).

Intermediate 15

3-(3-fluoro-4-(pyrrolidin-1-ylmethyl)phenyl)-3-hydroyxcyclobutanecarboxylic acid

n-Butyllithiumn (2.5 M/hexanes, 78 ml, 0.195 mol) was added down the reaction flask walls over 5 min to a −78° C. solution of 1-(4-bromo-2-fluorobenzyl)pyrrolidine (50.0 g, 0.194 mol) in THF (500 mL). After stirring at −78° C. for 1 h, a −78° C. solution of 3-oxocyclobutanecarboxylic acid (11.0 g, 96.4 mmol) in THF (150 mL) was cannulated over 10 min into the reaction mixture. The resulting dark orange solution was slowly warmed to room temperature over 16 h. LC/MS of the mixture showed the title compound 294.2 (M+H). This material was used as a crude solution without work-up, assuming ˜0.12 M concentration of the title compound.

EXAMPLE 18 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid methylamide

A THF solution of ˜0.12 M intermediate 15, 3-(3-fluoro-4-(pyrrolidin-1-ylmethyl)phenyl)-3-hydroxycyclobutanecarboxylic acid (160 mL, 19.3 mmol) was combined with methylamiine (2.0 M in THF, 20 mL, 40 mmol) and T3P (50% wt in EtOAc, 13.8 mL, 23.2 mmol) and stirred at room temperature for 20 h. The mixture was made basic with saturated aqueous NaHCO₃ and EtOAc (50 mL) was added. The phases were separated and the aqueous phase was extracted again with EtOAc. The combined organics were washed with brine, dried over MgSO₄ and concentrated to yield an orange oil (8.6 g). Flash chromatography on a 2⁰×4″ silica gel column flushing first with EtOAc (1L) and 10% MeOH/EtOAc (500 mL) to remove higher Rf impurnties followed by elution with an additional 500 mL of 10% MeOH/EtOAc and 500 mL 20% MeOH/EtOAc afforded 3.14 g (53%) of the title compound as a thick orange oil which slowly solidified to a waxy solid R_(f)=0.30, 20% MeOH/EtOAc; ¹H NMR (CDCl₃) δ 7.35 (t, J=7.7 Hz, 1H), 7.21-71.3 (m, 2H), 5.73 (br s, 1H), 3.66 (d, J_(H−F)=1.2 Hz, 2H), 2.86 (d, J=4.6 Hz, 3H), 2.85-2.73 (m, 3H), 2.55-2.45 (m, 6H), 1.79-1.60 (m overlapping water, 4H); ¹³C NMR (CDCl₃) δ 177.5, 161.3 (d, J_(C−F)=246.2 Hz). 147.3 (d, J_(C−F)=7.1 Hz), 131.6 (d, J_(C−F)=4.9 Hz), 124.2 (d, J_(C−F)=15.0 Hz), 120.6 (d, J_(C−F)=3.3 Hz), 112.3 (d, J_(C−F)=23.3 Hz), 74.0 (d, J_(C−F)=1.9 Hz), 54.04, 52,6 (d, J_(C−F)=1.5 Hz), 41.2, 32.9, 26.8, 23.6; LRMS m/z Calcd for C₁₇H₂₃FN₂O₂, 306.4, found, 307.4 (M+H) APCl.

EXAMPLE 19 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid dimethylamide

A THF solution of ˜0.12 M intermediate 15, 3-(3-fluoro-4-(pyrrolidin-1-ylmethyl)phenyl)-3-hydroxycyclobutanecarboxylic acid (160 mL, 19.3 mmol) was combined with dimethylamine (2.0 M in THF, 20 mL, 40 mmol) and T3P (50% in EtOAc, 13.8 mL, 23.2 mmol) and stirred at room temperature for 20 h. The mixture was made basic with saturated aqueous NaHCO₃ and EtOAc (50 mL) was added. The phases were separated and the aqueous phase was extracted again with EtOAc. The combined organics were washed with brine, dried over MgSO₄ and concentrated to yield an orange oil (8.6 g). Flash chromatography on a 2″×4″ silica gel column flushing first with EtOAc (1L) and 10% MeOH/EtOAc (500 mL) to remove higher Rf impurities followed by elution with an additional 500 mL of 10% MeOH/EtOAc and 500 mL 20% MeOH/EtOAc afforded 3.58 g (58%) of the title compound as a thick orange oil which slowly solidified to a waxy solid: R_(f)=0.17 (20% MeOH/EtOAc); ¹H NMR (CDCl₃) δ 7.36 (t, J=7.9 Hz, 1H), 7.23-7.15 (m, 2H) 4.70 (br s, 1H), 3.66 (s, 2H), 3.21-3.12 (m, 1H), 3.00 (3, 3H), 2.99 (s, 3H), 2.84-2.78 (m, 2H), 2.57-2.43 (m, 6H), 1.80-1.74 (m overlapping water, 4H), ¹³C NMR (CDCl₃) δ 175.8, 161.3 (d, J_(C−f)=246.2 Hz), 147.4 (d, J_(C−F)=7.1 Hz), 131.6 (d, J_(C−F)=4.5 Hz), 124.3 (d, J_(C−F)=15 Hz), 120.7 (d, J_(C−F)=3.0 Hz), 112.4 (d, J_(C−F)=23.3 Hz), 73.3 (d, J_(C−F)=1.1 Hz), 54.03, 52.6 (d, J_(C−F)−1.1 Hz), 41.1, 37.4, 36.2, 28.5, 23.6; LRMS m/z Calcd for C₁₈H₂₅FN₂O₂, 320.4, found, 321.4 (M+H) APCl.

EXAMPLE 20 3-(3-Fluoro-4pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid isobutyl-amide

A THF solution of crude ˜0.12 M internmediate 15, 3-(3-fluoro-4-pyrrolidin-1-ylmethyl)phenyl)-3-hydroxycyclobutanecarboxylic acid (160 mL, 19.3 mmol) was combined with isobutylamine (3.8 mL, 38.2 mmol) and T3P (50% wt in EtOAc, 13.8 mL, 23.2 mmol) and stirred at room temperature for 20 h. The mixture was made basic with saturated aqueous NaHCO₃ and EtOAc (50 mL) was added. The phases were separated and the aqueous phase was extracted again with EtOAc. The combined organics were washed with brine, dried over MgSO₄ and concentrated to yield an orange oil. Flash chromatography on a 2″×4″ silica gel column flushing first with EtOAc (1L) and 10% MeOH/EtOAc (500 mL) to remove higher Rf impurities followed by elution with an additional 500 mL of 10% MeOH/EtOAc and 500 mL 20% MeOH/EtOAc afforded 4.22 g (63%) of the title compound as an waxy, orange solid: R_(f)=0.3 (30% MeOH/EtOAc); ¹H NMR (CDCl₃) δ 7.34 (t, J=7.9 Hz, 1H), 7.20-7.13 (m, 2H), 5.84 (br s, 1H), 3.66 (d, J_(H−F)=1.3 Hz, 2H), 3.11 (t, J=6.4 Hz, 2H), 2.84-2.76 (m, 3H), 2.55-2.45 (m, 6H), 1.81-1.72 (m, 4H), 0.91-0.87 (d @ 0.90 (J=6.6 Hz, 6H) overlapping m (1H)); LRMS m/z Calcd for C₂₀H₂₉FN₂O₂, 348.5, found, 349.4 (M+H) APCl.

EXAMPLE 21 3-Fluoro-3-(3-fluoro-4-pyrroilidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide

Bis(2-methoxyethyl)aminosulfur trifluoride (0.29 mL, 1.57 mmol) was added to a 0° C., solution of Example 18, 3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid methylamide (0.40 g, 1.31 mmol) in CH₂Cl₂ (8 mL). This mixture was slowly warmed to room temperature and stirred for 18 h and then poured into saturated aqueous NaHCO₃. The organic phase was separated, dried over MgSO₄ and concentrated to yield an orange oil (0.40 g). Flash chromatography on a 1.5″×2″ silica gel column flushing first with 200 mL each EtOAc, 2% and 5% MeOH/EtOAc to remove higher Rf impurities followed by elution with 400 mL of 10% MeOH/EtOAc and 200 mL 20% MeOH/EtOAc afforded 0.244 g (61%) of the title compound as an orange oil: R_(f)=0.11 (20% MeOH/EtOAc).

The HCl salt of the title compound was prepared in EtOAc with 1.5 equivalent of 2N HCl/ethyl ether. The hygroscopic white solid was collected and dried under nitrogen: ¹H NMR (MeOH-d₄) δ 7.60 (t, J=7.9 Hz, 1H), 7.45-7.40 (m, 2H), 4.46 (s, 2H), 3.60-3.45 (m, 2H), 3.37 (p, J=8.7 Hz, 1H), 3.24-3.14 (m, 2H), 2.87-2.67 (s @ 2.72 (3H) overlapping a multiplet (4H)), 2.22-2.10 (m, 2H), 2.02-1.97 (m, 2H), ¹³C NMR (MeOH-d₄) δ 175.6, 161.5 (d, J_(C−F)=248.8 Hz), 147.4 (dd, J_(C−F)=24.1, 7.1), 133.1 (d, J_(C−F)=2.6 Hz), 121.4 (d, J_(C−F)=4.9 Hz), 117.6 (d, J_(C−F)=15.8 Hz); 112.4 (dd, J_(C−F)=23.3, 8.9 Hz), 96.4 (d, J_(C−F)=195.4 Hz), 54.0, 51.0, 38.3 (d, J_(C−F)=25.2 Hz), 32.2, 25.3, 22.7; LRMS m/z Calcd for C₁₇H₂₂F₂N₂O, 308.4, found, 309.4 (M+H) APCl.

EXAMPLE 22 3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid dimethylamide

A solution of example 19, 3-(3-fluoro-4-pyrroldin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid dimethylamide (0.40 g, 1.25 mmol) in CH₂Cl₂ (4 mL) was added to a −78° C. solution of bis(2-methoxyethyl)aminosulfur trifluoride (0.28 mL, 1.52 mmol) in CH₂Cl₂ (4 mL). After 1 h, an additional portion of bis(2-methoxyethyl)aminosulfur trifluoride (0.050 mL) was added and the solution was stirred for an additional 15 min then saturated aqueous NaHCO₃ was added and the mixture was stirred at room temperature for 16 h. The phases were separated and the aqueous layer was extracted with CH₂Cl₂. The combined organics were dried over MgSO₄ and concentrated to yield 296 mg of a light orange oil. Flash chromatography on a 1.5″×1.5″ silica gel column flushing first with 200 mL EtOAc to remove higher Rf impurities followed by elution with 200 mL of 20% MeOH/EtOAc afforded 0.244 g (61%) of the title compound as a light yellow oil: R_(f)=0.10 (20% MeOH/EtOAc).

The HCl salt of the title compound was prepared in EtOAc with 1.5 equivalents of 2N HCl/ethyl ether to afford a white solid: ¹H NMR (MeOH-d₄) δ 7.59 (t, J=7.9 Hz, 1H), 7.38-7.32 (m, 2H), 4.44 (s, 2H), 3.75 (p, J=8.7 Hz, 1H), 3.55-3.45 (m, 2H), 3.22-3.15 (m, 2H), 3.01 (s, 3H), 2.92 (s, 3H), 2.84-2.80 (m, 2H), 2.76 (d, J=8.7 Hz, 2H), 2.21-2.09 (m, 2H), 2.05-1.90 (m, 2H); ¹³C NMR (MeOH-d₄) δ 174.2, 161.5 (d, J_(C−F)=248.8 Hz), 147.4 (dd, J_(C−F)=24.1. 7.5 Hz), 133.1 (d, J_(C−F)=3.0 Hz), 121.3 (dd, J_(C−F)=7.9, 3.2 Hz), 117.6 (d, J_(C−F)=15.4 Hz), 112.3 (dd, J_(C−F)=23.3, 9.0 Hz), 95.9 (dd, J_(C−F)=197.3, 2.1 Hz) 53.9, 50.7 (d, J_(C−F)=3.0 Hz), 38.1 (d, J_(C−F)=24.8 Hz), 36.0, 34.8, 29.8, 22.6; LRMS m/z Calcd for C₁₈H₂₄F₂N₂O, 322.4, found, 323.4 (M+H) APCl.

EXAMPLE 23 3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethyl-methyl-amide

A solution of example 14, 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid ethyl-methyl-amide (0.40 g, 1.20 mmol) in CH₂Cl₂ (4 ml) was added to a −78° C. solution of bis(2-methoxyethyl)aminosulfur trifluioride (0.27 mL, 1.46 mmol) in CH₂CL₂ (4 mL). After 1 h, saturated aqueous NaHCO₃ (10 mL) was added and the mixture was stirred at room temperature for 16 h. The phases were separated and the aqueous layer was extracted with CH₂Cl₂. The combined organics were dried over MgSO₄ and concentrated to yield 296 mg of a light orange oil. Flash chromatography on a 1.5°×1.5″ silica gel column flushing first with 200 mL EtOAc to remove higher Rf impurities followed by elution with 200 mL each of 10% and 20% MeOH/EtOAc afforded 0.242 g (61%) of the title compound as a light yellow oil: R_(f)=0.24 (20% MeOH/EtOAc); LRMS m/z Calcd for C₁₉H₂₆F₂N₂O, 336.4, found, 337.4 (M+H), 317.4 (M+H−HF) APCl.

The HCl salt of the title compound was prepared in EtOAc with 1.5 equivalents of 2N HCl/ehtyl ether to afford a light yellow solid: ¹H NMR (MeOH-d₄) δ˜1:1 mixture of rotamers, 7.58 (t, J=7.7 Hz, 1H), 7.40-7.33 (m, 2H), 4.44 (s, 2H), 3.79-3.67 (m, 1H), 3.49 (br S, 2H), 3.42-3.34 (m, 2H), 3.21 (br s, 2H), 2.98 and 2.90 (singlets, 3H total), 2.86-2.74 (m, 4H), 2.23-1.90 (br m, 4H), 1.18 and 1.07 (triplets, J=7.1 Hz, 3H total); ¹³C NMR (MeOH-d₄) δ (mixture of rotamers) 173.87, 173.65, 162.73, 180.26, 147.56, 147.49, 147.33, 147.25. 133.11, 133.08, 121.34, 121.31, 121.26, 121.23, 117.74, 117.59, 112.48, 112.39, 112.25, 112.16, 97.12, 96.89, 95.18, 94.93, 53.88, 50.75, 50.72, 44.12, 42.75, 38.55, 38.30, 38.19, 37.94, 33.66, 32.21, 29.97, 29.46, 22,62, 12.86, 11.25; LRMS m/z Calcd for C₁₉H₂₈F₂N₂O, 336.4, found, 337.4 (M+H) APCl.

EXAMPLE 24 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-methoxy-cyclobutanecarboxylic acid ethyl-methyl-amide

Sodium hydride (60% wt, 0.040 g, 1.00 mmol) was added to a solution of Example 14, 3-(3Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid ethyl-methyl-amide (0.25 g, 0.748 mmol) in THF (5 mL). After stirring for 15 min, all gas evolution ceased and methyliodide (0.06 mL, 0.96 mmol) was added. The resulting mixture was stirred at room temperature for 16 h then quenched with water and extracted into EtOAc. The extract was washed with brine, dried over MgSO₄ and concentrated to give the title compound as a light yellow oil (0.13 g): R_(f)=0.16 (20% MeOH/EtOAc); LRMS m/z Calcd for C₂₀H₂₉FN₂O₂, 348.5, found, 349.4 (M+H) APCl.

The HCl salt of the title compound was prepared in EtOAc with 1.5 equivalents of 2N HCl/ethyl ether to afford a white solid: ¹H NMR (MeOH-d₄) δ ˜1:1 mixture of rotamers, 7.67 (t, J=7.7 Hz, 1H), 7.46 (d, J=8.3 Hz, 1H), 7.42 (dt, J=11.2, 1.9 Hz, 1H), 4.48 (s, 2H), 3.55 (br s, 2H), 3.42-3.18 (multiplets overlapping with MeOH, 4H), 3.02-2.95 (m, 1H), 2.94-2.90 (overlapping —OCH₃ singlet @2.94 and optometric —NCH₃ singlets @ 2.94 and 2.90, 6H total), 2.67-2.54 (m, 4H), 2.18 (br s, 2H), 2.03 (br s, 2H), 1.13 and 1.08 (triplets, J=7.3 Hz, 3H total); ¹³C NMR (MeOH-d₄) δ (mixture of rotamers) 173.93, 173.84, 163.09, 160.61, 148.64, 148.57, 133.20, 133.18, 123.02, 117.39, 117.20, 114.13, 114.08, 113.91, 133.86, 76.72, 76.63, 53.88, 50.80, 50.77, 49.78, 43.97, 42.76, 36.58, 36.24, 33.64, 32.17, 27.87, 27.32, 22.67, 12.87, 11.28; LRMS m/z Calcd for C₂₀H₂₉FN₂O₂, 348.5, found, 349.4 (M+H) APCl.

Intermediate 16

3-(3-fluoro-4-((pyrrolidin-1-yl)methyl)phenyl)-N-isobutylcyclobut-2-enecarboxamide

A solution of Example 20, 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid isobutyl-amide (3.71 g, 10.64 mmol) in trifluoroacetic acid (20 mL) and 1,2-dichloroethane (120 mL) was refluxed for 21 h and concentrated to give 8.6 g of crude 3-(3-fluoro-4-(pyrrolidin-1-ylmethyl)phenyl)-N-isobutylcyclobut-2-enecarboxamide trifluoroacetate salt and residual TFA as a dark red-brown oil. Diagnostic ¹H NMR signals: (CDCl₃) δ 7.48 (t, J=3 7.7 Hz, 1H), 7.23 (d, partially obscured by CHCl₃ signal, 1H), 7.09 (dd, J=10.3, 1.5 Hz, 1H), 6.38 (d J=0.8 Hz, 1H), 4.33 (d, J=5.0 Hz, 1H), 3.77 (br s, 2H), 3.68 (d, J=4.6 Hz, 1H), 3.20-3.07 (m, 3h), 2.98 (br s, 2H), 2.83 (dd, J=13.3, 1.7 Hz, 1H), 2.19-2.07 (m, 4H), 0.90 (d, J=7.1 H, 6H).

EXAMPLE 25 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid isobutyl-amide

Crude intermediate 16, 3-(3-fluoro-4-((pyrrolidin-1-yl)methyl)phenyl)-N-isobutylcyclobut-2-enecarboxamide prepared above was dissolved in EtOH (100 mL) and added to a hydrogenation bottle containing 10% palladium on carbon slurried in EtOH (˜5 mL). The mixture was shaken at room temperature under 45 psi of hydrogen for 2 h, filtered through diatomaceous earth with an EtOH rinse and concentrated to yield an orange oil. This was dissolved in EtOAc (150 mL) and washed with aqueous K₂CO₃ and brine, dried over MgSO₄ and concentrated to afford 3.29 g of waxy orange solid. Flash chromatography on a 2.5″×4″ silica gel columnn eluting with 1000 mL EtOAc and 500 mL 10% MeOH/EtOAc afforded 1.90 g (54%) of the title compound as a yellow tinged solid: R_(f)=0.26 (20% MeOH/EtOAc).

The HCl salt of the title compound was prepared by addition of ˜1.2 eq. of 2N HCl/ethyl ether to the free base in EtOAc solution. The resulting hygroscopic, glassy, light orange solid had; ¹H NMR (MeOH-d₄) δ 7.49 (t, J=8.1 Hz, 1H), 7.20-7.17 (m, 2H), 4.41 (s, 2H), 3.55-3.45 (m, 3H), 3.21-3.15 (m, 2H), 3.09 (p, 8.7 Hz, 1H), 2.98 (d, J=6.6 Hz, 2H), 2.57-2.50 (sym. mult., 2H), 2.31 (dq, J=9.7, 2.5 Hz, 2H), 2.22-2.10 (m, 2H), 2.05-1.95 (m, 2H), 1.75 (hept, J=6.8 Hz, 1H), 0.88 (d, J=6.6 Hz, 6Hz); ¹³C NMR (CDCl₃) δ 176.1, 161.64 (d, J_(C−F)=248.1 Hz), 150.7 (d, J_(C−F)=7.9 Hz), 132.8 (d, J_(C−F)=3.4 Hz), 123.2 (d, J_(C−F)=3.0 Hz), 115.7 (d, J_(C−F)=5.4 Hz), 113.8 (dd, J_(C−F)=21.8 Hz), 53.73, 50.9, 50.8, 35.2, 34.9, 32.5, 28.4, 22.6, 19.3; LRMS mn/z Calcd for C₂₀H₂₉FN₂O, 332.5, found, 333.5 (M+H) APCl.

EXAMPLE 26 3-aza-bicyclo[3.2.2]nonan-3-yl(3-(3-fluoro-4-(pyrrolidin-1-ylmethyl)phenyl-3- hydroxycyclobutyl)methanone

A THF solution of crude intermediate 15, 3-(3-fluoro-4-(pyrrolidin-1-ylmethyl)phenyl)-3-hydroxycyclobutanecarboxylic acid (˜5.3 mmol, ˜0.12M THF) was combined with 3-aza-bicyclo[3.2.2]nonane (1.00 g, 7.99 mmol) and T3P (50% wt in EtOAc, 3.8 mL, 6.38 mmol) and stirred at room temperature for 30 min. The mixture was made basic with saturated aqueous NaHCO₃ and then EtOAc (50 mL) was added. The phases were separated and the aqueous phase was extracted again with EtOAc. The combined organic layers were washed with brine, dried over MgSO₄ and concentrated to yield a thick orange oil (1.86 g). Flash chromatography on a 2″×5″ silica gel column flushing first with EtOAc (500 mL) followed by elution with 500 mL 25% MeOH/EtOAc afforded the title compound (0.59 g 28% yield) as a waxy yellow solid: LRMS m/z Calcd for C₂₄H₃₃FN₂O₂, 400.5, found, 401.1 (M+H) APCl; ¹H NMR (CDCl₃) δ 7.40 (t, J=7.5 Hz, 1H), 7.23-7.17 (m, 2H), 3.75 (d, J=4.6 Hz, 2H), 3.69 (s, 2H), 3.29 (d, J=3.7 Hz, 2H), 3.25-3.19 (m, 1H), 2.84-2.78 (m, 2H), 2.62-2.45 (m, 6H), 2.10-2.08 (m, 1H), 2.03-2.00 (m, 1H) 1.93-1.40 (m, 12H).

EXAMPLE 27 3-aza-bicyclo[3.2.2]nonan-3-yl(3-(3-fluoro-4-pyrrolidin-1- ylmethyl)phenyl)cyclobutyl)methanone

A solution of example 26, 3-aza-bicyclo[3.2.2]nonan-3-yl(3-(3-fluoro-4-(pyrrolidin-1-ylmethyl)phenyl)-3-hydroxycyclobutyl)methanone (0.59 g, 1.48 mmol) in trifluoroaceticacid (2.5 mL) and 1,2-dichloroethane (16 mL) was refluxed for 20 h and concentrated to give crude 3-aza-bicyclo[3.2.2]nonan-3-yl(3-(3-fluoro-4-(pyrrolidin-1-ylmethyl)phenyl)cyclobut-2-enyl)methanone trifluoroacetate salt as a dark purplish-brown oil, with residual TFA. This material was dissolved in EtOH (40 mL) and added to a hydrogenation bottle containing 10% palladium on carbon (93 mg) slurried in EtOH (˜3 mL). The mixture was shaken at room temperature under 48 psi of hydrogen for 2 h, filtered through diatomaceous earth with an EtOH rinse and concentrated to yield an orange oil. This was dissolved in EtOAc and washed with aqueous K₂CO₃ and brine, dried over MgSO₄ and concentrated to afford 0.38 g of light orange oil. Flash chromatography on a 2″×3.5″ silica gel column flushing with 2% MeOH/EtOAc (500 mL) and then eluting with 500 ml each 5% and 10% MeOH/EtOAc afforded 0.256 g (45%) of the title compound as a light orange oil: R_(t)=0.21 (20% MeOH/EtOAc).

The HCl salt was prepared by addition of ˜1.5 eq. of 2N HCl/ethyl ether to the free base in EtOAc solution. The resulting white solid was collected, and dried to give the HCl salt of the title compound: ¹H NMR (CDCl₃) δ 12.68 (br s, 1H), 7.80 (t, J=7.7 Hz, 1H), 7.19-6.97 (m, 2H), 4.21 (d, J=4.2 Hz, 2H), 3.80-3.56 (m, 4H), 3.55-3.37 (m, 3H), 3.28 (p, J=8.9 Hz, 1H), 2.83 (br s, 2H), 2.61-2.54 (m, 2H), 2.47-2.38 (m, 2H), 2.32-2.12 (m, 2H), 2.12-1.92 (m, 4H), 1.77-1.54 ( 8H); ¹⁸C NMR (CDCl₃) δ 173.4, 161.5 (d, J_(C−F)=248.4 Hz), 150.6 (d, J_(C−F)=7.5 Hz), 133.6 (d, J_(C−F)=2.3 Hz), 124.0 (d, J_(C−F)=3.0 Hz), 114.3 (d, J_(C−F)=13.9 Hz), 113.8 (d, J_(C−F)=21.8 Hz), 54.3, 52.7, 50.3, 50.03, 50.0, 35.2, 33.9, 33.0, 30.4, 30.0, 25.0, 24.7, 23.3; LRMS m/z Calcd for C₂₄H₃₃FN₂O, 384.5, found, 385.5 (M+H) APCl.

Intermediate 17

3-(3-chloro-4-((pyrrolidin-1-yl)methyl)phenyl)-3-hydroxycyclobutanecarboxylic acid

A 2.5 M solution of n-BuLi in hexanes (101 mL, 254 mmol) was added over 15 min to a solution of 1-(4-bromo-2-chlorobenzyl)pyrrolidine (69.6 g, 254 mmol) in absolute THF (450 ml) under a flow of nitrogen at −78° C. The reaction mixture was stirred at −78° C. for 30 min. Then a −78° C. chilled solution of 3-oxocyclobutanecarboxylic acid (14.4 g, 126.7 mmol) in absolute THF (150 ml) was added drop wise for 10 min at −78° C. The mixture was warmed to RT slowly and left stirring for 18 hrs and the resulting solution was used. LRMS m/z Calcd for C₁₈H₂₀NClO₃, 309.8, found, 308.1 (M−H) APCl.

EXAMPLE 28 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid methylamide

To a crude solution of intermediate 17, 3-(3chloro-4-((pyrrolidin-1-yl)methyl)phenyl)-3-hydroxycyclobutanecarboxylic acid (˜666 mL, ˜121.5 mmol) was added 2.0 M methylamine (95 mL, 190 mmol, in THF) and T₃P (50 wt % solution in EtOAc, 96.6 mL, 152 mmol). The resulting reaction mixture was stirred at RT for 1 hr and then 300 ml of 1N NaOH and 400 mL of EtOAc were added and the layers were separated. The aqueous layer was subjected to EtOAc extraction (2×500 ml) again and the combined organic layers were dried over MgSO₄ and evaporated. The residue wvas purified by flash column chromatography using a 75 L Biotage™ column, eluting with gradients of 5%, 8%, 10%, 15% MeOH/CH₂Cl₂ with 0.25% NH₄OH. The product containing fractions were collected and concentrated under reduced pressure to give the title compound (18.9 g, 48% yield). R_(f)=0.35 (20% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C17 H23 Cl N2 O2, 322.2, found, 323.1 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) δ 7.47-7.45 (m, 2H), 7.33 (dd, J=7.9, 1.7 Hz. 1H) 5.94 (brs, 1H), 5.67 (brs, 1H), 3.75 (s, 3H), 2.85 (d, J=4.9 Hz, 3H), 2.86-2.73 (m, 3H), 2.62-2.56 (m, 4H), 2.54-2.47 (m, 2H), 1.84-1.76 (m 4H); 100 MHz ¹³C NMP (CDCl₃) δ 177.6, 146.0, 135.5, 134.0, 130.8, 126.3, 123.6, 74.3, 56.8, 54.3, 41.1, 33.3, 26.9, 23.7.

EXAMPLE 29 AND EXAMPLE 30 Trans-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide and Cis-3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)- cyclobutanecarboxylic acid methylamide

TFA (48 m, 627 mmol) was added to a DCE solution (202 ml) of example 28, 3-(3-chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid methylamide (10 g, 31.4 mmol) and the resulting mixture was heated to 75° C. for 18 hrs and concentrated down to obtain the TFA salt of 3-(3-chloro-4-(pyrrolidin-1-yl) methyl) phenyl)-N-methylcyclobut-2-enecarboxamide. This was redissolved in absolute EtOH (130 ml), Wilkinson's catalyst (1.5 g) was then added and the reaction mixture was subjected to hydrogenation at 60° C. using 45 psi H₂. After 2 hr reaction time, it was concentrated down and the residue was redissolved into 1N HCl (100 ml) and extracted twice with EtOAc (2×100 ml). The aqueous layer was then basified with 1N NaOH (100 ml) and extracted with EtOAc (2×500 ml). The combined organic phases were dried over MgSO₄, and concentrated down to obtain crude material. This was purified by flash chromatography using a 120 g ISCO™ column eluting with a gradient of 5%, 10% and 15% MeOH/CH₂Cl₂ with 0.25% NH₄OH. The product containing fractions were combined and concentrated under reduced pressure to give a mixture of cis and trans isomers (4.6 g, 48% yield). The isomers were separated by preparative chromatography on a Chiralcel OD (10 cm×50 cm) column at a flow-rate of 295 ml/min and using Heptane/IPA (90/10) as eluent to recover trans (3.6 g) and cis (0.52 g) isomers.

EXAMPLE 29

Trans-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide: R_(f)0.50 (20% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C17 H23 Cl N2 O, 306.8, found, 307.4 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) δ 7.42 (d, J=7.8 Hz, 1H), 7.20 (d, J=1.6 Hz, 1H), 7.09 (dd, J=7.8, 1.2 Hz, 1H), 5.76 (brs, 1H), 3.83 (s, 2H), 3.76-3.67 (m, 1H), 2.98-2.90 (m, 1H), 2.82 (d, J=5.1 Hz, 3H), 2.80-2.62 (m, 6H), 2.36-2.26 (m, 2H), 1.87-1.78 (m, 4H); 100 MHz ¹³C NMR (CDCl₃) δ 146.7, 134.2, 131.3, 176.1, 127.5, 125.3, 56.3, 54.3, 54.1, 36.5, 36.3, 32.1, 26.5, 23.6.

EXAMPLE 30

Cis-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide; R_(f)=0.50 (20% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C17 H23 Cl N2 O, 306.8, found, 307.4 (M+1) APCl, 400 MHz ¹H NMR (CDCl₃) δ 7.30 (d, J=7.9 Hz, 1H), 7.15 (d, J=1.2 Hz, 1H), 7.03 (dd, J=7.8, 1.2 Hz 1H), 6.48 (brs, 1H), 3.63 (s, 2H), 3.30-3.19 (m, 1H), 2.94-2.84 (m, 1H), 2.70 (d, J=5.0 Hz, 3H), 2.52-2.26 (m, 8H), 1.74-1.66 (m, 4H).

EXAMPLE 31 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid dimethylamide

To a solution of crude intermediate 17, 3-(3-chloro-4-((pyrrolidin-1-ylmethyl)phenyl)-3-hydroxycyclobutanecarboxylic acid (˜4.3 mL ˜0.65 mmol) was added dimethylamnine in THF (0.65 ml, 1.29 mmol, 2.0M THF) and T₃P (50 wt % solution in EtOAc, 0.62 m, 0.97 mmol). The resulting reaction mixture was stirred at RT for 1 hr and then 25 ml of 1N NaOH and 100 ml of EtOAc were added and the layers were separated. The aqueous layer was subjected to EtOAc extraction (2×60 ml) again and the combined organic layers were dried over MgSO₄ and evaporated. The residue was purified by flash column chromatography using a 40 g ISCO™ column, eluting with 5% MeOH/CH₂Cl₂ containing 0.25% NH₄OH. The product containing fractions were collected and concentrated under reduced pressure to give the title compound (112 mg, 52% yield). R_(f)=0.65 (20% MeOH/CH₂CH₂+0.2% NH₄OH); LRMS m/z Calcd for C18 H25 Cl N2 O2, 336.8, found, 337.1 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) δ (d, J=1.7 Hz, 1H), 7.46, 7.40 (d, J=7.9 Hz, 1H), 7.33 (dd, J=7.9, 1.7Hz, 1H), 3.68 (s, 2H), 3.06-2.96 (m, 1H); 2.92 (s, 6H), 2.78-2.68 (m, 2H), 2.62-2.46 (m, 6H): 1.76-1.68 (m, 4H); 100 MHz ¹³C NMR (CDCl₃) δ 175.5, 146.2, 135.5, 133.9, 130.7, 126.5, 123.7, 72.9, 56.8, 54.3, 40.9, 37.4, 36.1, 28.5, 23.7.

EXAMPLE 32 [3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutyl]-piperidin-1- yl-methanone

To a solution of crude intermediate 17, 3-(3-chloro-4-((pyrrolidin-1-yl)methyl)phenyl)-3-hydroxycyclobutanecarboxylic acid (˜4.3 mL ˜0.65 mmol) was added piperidine (0.13 ml, 1.29 mmol) and T₃P (50 wt % solution in EtOAc, 0.62 ml, 0.97 mmol). The resulting reaction mixture was stirred at RT for 1 hr and then 25 ml of 1N NaOH and 100 ml of EtOAc were added and the layers were separated. The aqueous layer was subjected to EtOAc extraction (2×60 ml) again and the combined organic layers were dried over MgSO₄ and evaporated. The residue was purified by flash column chromatography using a 40 g ISCO™ column, eluting with 5% MeOH/CH₂Cl₂ containing 0.25% NH₄OH. The product containing fractions were collected and concentrated under reduced pressure to give the title compound (113 mg, 46% yield). R_(f)=0.80 (20% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C21 H29 Cl N2 O2, 376.9, found, 377.1 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) δ 7.47 (d, J=2.1 Hz, 1H), 7.42 (d, J=7.9 Hz, 1H), 7.34 (dd, J=7.9, 1.7 Hz, 1H), 3.70 (s, 2H), 3.54-3.48 (m, 2H), 3.32-3.26 (m, 2H), 3.02-2.93 (m, 1H), 2.75-2.67 (m, 2H), 2.65-2.51 (m, 6H), 1.78-1.71 (m, 4H), 1.63-1.44 (m, 6H), 100 MHz ¹³C NMR (CDCl₃) δ 173.5, 146.3, 135.4, 133.9, 130.8, 126.5, 123.7, 72.9, 56.7, 54.3, 46.8, 43.5, 41.0, 28.4, 26.8, 25.8, 24.7, 23.7.

EXAMPLE 33 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid isobutyl-methyl-amide

To a solution of crude intermediate 17, 3-(3-chloro-4-((pyrrolidin-1-yl)methyl)phenyl)-3-hydroxycyclobutanecarboxylic acid (˜4.3 mL ˜0.65 mmol) was added N-methylisobutylamine (0.15 ml, 1.29 mmol) and T₃P (50 wt % solution in EtOAc, 0.62 ml, 0.97 mmol). The resulting reaction mixture was stirred at RT for 1 hr and then 25 ml of 1N NaOH and 100 ml of EtOAc were added and the layers were separated. The aqueous layer was subjected to EtOAc extraction (2×60 ml) again and the combined organic layers were dried over MgSO₄ and evaporated. The residue was purified by flash column chromatography using a 40 g ISCO™ column, eluting with 5% MeOH/CH₂Cl₂ containing 0.25% NH₄OH. The product containing fractions were collected and concentrated under reduced pressure to give the title compound (108 mg, 44% yield). R_(f)=0.80 (20% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C21 H31 Cl N2 O2, 378.9, found, 379.1 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) 1:1 mixture of rotomers, δ 7.47-7.45 (m, 1H), 7.42 (d, J=7.9 Hz, 1H), 7.36-7.30 (m, 1H), 3.71 (s, 2H), 3.20-3.02 (m, 3H), 2.92 & 2.90 (2s, 3H total), 2.80-2.70 (m, 2H), 2.60-2.50 (m, 6H), 1.95-1.80 (m, 1H), 1.78-1.70 (m, 4H), 0.76-0.58 (m, 6H); 100 MHz ¹³C NMR (CDCl₃) 1:1 mixture of rotomers, line list δ 176.6, 175.8, 146.4, 135.2, 135.1, 134.0, 130.9, 130.8, 126.4, 123.7, 123.6, 73.5, 73.3, 57.9, 56.7, 55.6, 54.3, 41.4, 41.0, 36.1, 35.0, 29.0, 28.2, 27.9, 26.9, 23.7, 20.2, 20.1.

EXAMPLE 34 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid cyclopropylmethyl-amide

To a solution of crude intermediate 17, 3-(3-chloro-4-((pyrrolidin-1yl)methyl)phenyl)-3-hydroxycyclobutanecarboxylic acid (˜4.3 mL ˜0.65 mmol) was added aminomethylcyclopropane (0.112 ml, 1.29 mmol) and T₃P (50% solution in EtOAc, 0.62 ml, 0.97 mmol). The resulting reaction mixture was stirred at RT for 1 hr and then 25 ml of 1N NaOH and 100 ml of EtOAc were added and the layers were separated. The aqueous layer was subjected to EtOAc extraction (2×60 ml) again and the combined organic layers were dried over MgSO₄ and evaporated. The residue was purified by flash column chrotmatography using a 40 g ISCO™ columnn, eluting with 5% MeOH/CH₂Cl₂ containing 0.25% NH₄OH. The product containing fractions were collected and concentrated under reduced pressure to give the title compound (101 mg, 43% yield). R_(f)=0.80 (20% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C20 H27 Cl N2 O2, 362.9, found, 363.2 (M+1) APCl; 400 MH_(z) ¹H NMR (CDCl₃) δ 7.44-7.38 (m, 2H), 7.28 (dd, J=7.9, 2.6 Hz, 1H), 6.40 (br apt t, J=5.4 Hz, 1H), 3.69 (s, 2H), 3.10-3.04 (m, 2H), 2.76-2.68 (m, 3H), 2.57-2.42 (m, 6H), 1.78-1.69 (m, 4H), 0.94-0.84 (m, 1H), 0.48-0.40 (m, 2H), 0.18-0.12 (m, 2H), 100 MHz ¹³C NMR (CDCl₃) δ 176.7, 146.1, 135.3, 133.9, 130.8, 126.3, 123.6, 74.0, 56.7, 54.3, 44.9, 41.1, 40.7, 33.1, 23.7, 10.8, 3.7.

EXAMPLE 35 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid methyl-(tetrahydro-pyran-4-ylmethyl)-amide

To a solution of crude intermediate 17, 3-(3-chloro-4-((pyrrolidin-1-yl)methyl)phenyl)-3-hydroxycyclobutanecarboxylic acid (˜4.3 mL ˜0.65 mmol) was added methyl-(tetrahydro-pyran-4-ylmethyl-amine hydrochloride (200 mg, 1.21 mmol), triethylamine (0.108 ml, 0.78 mmol) and T₃P (50% solution in EtOAc, 0.62 ml, 0.97 mmol). The resulting reaction mixture was stirred at rt for 1 hr and then 25 ml of 1N NaOH and 100 ml of EtOAc were added and the layers were separated. The aqueous layer was subjected to EtOAc extraction (2×60 ml) again and the combined organic layers were dried over MgSO₄ and evaporated. The residue was purified by flash column chromatography using a 40 g ISCO™ column, eluting with 5% MeOH/CH₂Cl₂ containing 0.25% NH₄OH. The product containing fractions were collected and concentrated under reduced pressure to give the title comnpound (87 mg, 32% yield). R_(f)=0.80 (20% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C23 H33 Cl N2 O, 420.9, found, 421.3 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) mixture of rotomers, diagnostic peaks, δ 3.98-3.88 (m, 2H), 3.73 (s, 2H), 2.95 (s, 3H), 2.62-2.54 (m, 6H), 1.70-1.46 (m, 2H), 1.38-1.17 (m, 2H).

EXAMPLE 36 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid cyclopropylmethyl-methyl-amide

To a solution of crude intermediate 17, 3-(3-chloro-4-((pyrrolidin-1-yl)methyl)phenyl)-3-hydroxycyclobutanecarboxylic acid (˜4.3 mL ˜0.65 mmol) was added cyclopropylmethyl-methyl-amine hydrochloride (61 mg, 0.51 mmol) triethylamine (0.18 ml, 1.29 mmol) and T₃P (50% solution in EtOAc, 0.62 ml, 0.9 mmol). The resulting reaction mixture was stirred at RT for 1 hr and then 25 ml of 1N NaOH and 100 ml of EtOAc were added and the layers were separated. The aqueous layer was subjected to EtOAc extraction (2×60 ml) again and the combined organic layers were dried over MgSO₄ and evaporated. The residue was purified by flash column chromatography using a 40 g ISCO™ column, eluting 5% MeOH/CH₂Cl₂ containing 0.25% NH₄OH. The product containing fractions were collected and concentrated under reduced pressure to give the title compound (71 mg, 29% yield). R_(f)=0.50 (15% MeOH/CH₂]Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C21 H29 Cl N2 O2, 376.9, found, 377.2 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) 1:1 mixture of rotomers, diagnostic peaks, δ 3.75 (s, 2H), 3.02 & 3.01 (2 singlets, 3H total), 2.62-2.54 (m, 6H), 1.82-1.74 (m, 4H); 100 MHz ¹³C NMR (CDCl₃) 1:1 mixture of rotomers, peak list δ 175.9, 175.7, 146.4, 146.3, 135.2, 134.0, 130.9, 130.8, 126.5, 126.4, 123.7, 73.7, 73.5, 56.7, 54.6, 54.3, 52.4, 41.3, 41.0, 35.5, 34.5, 29.0, 28.5, 23.7, 10.5, 9.5, 3.8, 3.6.

EXAMPLE 37 [3-(3-Chloro-4-pyrrolidin-1-methyl-phenyl)-3-hydroxy -cyclobutyl[-(2,3-dihydro- 5H-benzo[f],[1,4]oxazepin4+L-YL)-METHANONE

To a solution of crude intermediate 17, 3-(3-chloro-4-((pyrrolidin-1-yl)methyl)phenyl)-3-hydroxycyclobutanecarboxylic acid (˜4.3 mL ˜0.65 mmol) was added 2,3,4,5-tetrahydro-benzo[f][1,4]oxazepine hydrochloride (239 mg, 1.29 mmol), triethylamine (0.18 ml, 1.29 mmol) and T₃P (50% solution in EtOAc, 0.62 ml, 0.97 mmol). The resulting reaction mixture was stirred at RT for 1 hr and then 25 ml of 1N NaOH and 100 ml of EtOAc were added and the layers were separated. The aqueous layer was subjected to EtOAc extraction (2×60 ml) again and the combined organic layers were dried over MgSO₄ and evaporated. The residue was purified by flash column chromatography using a 40 g ISCO™ column, eluting with 5% MeOH/CH₂Cl₂ containing 0.25% NH₄OH. The product containing fractions were collected and concentrated under reduced pressure to give the title compound 104 mg, 37% yield. R_(f)=0.50 (15% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C25 H29 Cl N2 O3, 440.9, found, 441.2 (M+1) APCl; 100 MHz ¹³C NMR (CDCl₃) 1:1 mixture of rotomers, diagnostic peaks, δ 175.4, 173.9, 159.5. 159.3, 134.2, 134.1, 131.4, 131.3, 122.0, 121.3, 121.0, 74.9, 72.6, 72.2, 56.3, 54.1, 53.9, 49.3, 48.7, 41.2, 41.0, 28.8, 28.5, 23.7, 23.6.

EXAMPLE 38 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid methyl-(3-methyl-pyridin-2-ylmethyl)-amide

To a solution of crude intermediate 17, 3(3-chloro-4-((pyrrolidin-1-yl)methyl)phenyl)-3-hydroxycyclobutanecarboxylic acid (˜4.3 mL ˜0.65 mmol) was added methyl-(3-methyl-pyridin-2-ylmethyl)-amine (176 mg, 1.29 mmol) and T₃P (50% solution in EtOAc, 0.62 ml, 0.97 mmol). The resulting reaction mixture was stirred at RT for 1 hr and then 25 ml of 1N NaOH and 100 ml of EtOAc were added and the layers were separated. The aqueous layer was subjected to EtOAc extraotion (2×60 ml) again and the combined organic layers were dried over MgSO₄ and evaporated. The residue was purified by flash column chromatography using a 40 g ISCO™ columnn, eluting with 5% MeOH/CH₂Cl₂ containing 0.25% NH₄OH. The product containing fractions were collected and concentrated under reduced pressure to give the title compound 127 mg, 46% yield). R_(f)=0.30 (15% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C24 H30 Cl N3 O2, 427.9, found, 428.2 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) 2:1 mixture of rotomers, diagnostic peaks, δ 4.72, 4.53 (s, 2H), 3.72 & 3.70 (s, 2H), 2.97, 2.93 (s, 3H), 1.76-1.74 (m, 4H); 100 MHz ¹³C NMR (CDCl₃) 2:1 mixture of rotomers, diagnostic peaks δ 177.6, 175.7, 154.7, 153.6, 146.8, 146.6, 126.5, 126.4, 122.8, 122.7, 73.4, 73.2, 56.7, 56.6, 54.3, 54.2, 51.1, 41.4, 40.9, 35.6, 35.4, 28.9, 28.7, 23.7, 23.6, 18.3, 18.2.

EXAMPLE 39 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid dimethylamide

TFA (9.9 ml, 128 mmol) was added to a DCE solution (64 ml) of example 19, 3-(3-fluoro-4-pyrrolidin-1-yl-methyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid dimethylamide (2.05 g, 6.4 mmol) and the mixture was heated to 75° C. for 18 hrs and concentrated down to obtain TFA salt of intermediate 3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobut-2-enecarboxylic acid dimethylamide. This was redissolved in absolute EtOH (64 ml), Wiilkinson's catalyst (296 mg) was then added and this mixture was subjected to hydrogenation at 60° C. using 45 psi H₂. After 1 hr 45 min reaction time, it was concentrated down and the residue was redissolved into 1HCl (50 m) and extracted twice with EtOAc (2×120 ml). The aqueous layer was then basified With 15% aq. NaOH (40 ml) and extracted with EtOAc (3×200 ml). The comnbined organic phases were dried over MgSO₄, and concentrated down to obtain crude material. This was purified by flash chromatography using a 120 g ISCO™ column and 4% MeOH/CH₂Cl₂ with 0.1% NH₄OH. The product containing fractions were combined and concentrated under reduced pressure to give the title compound (1.0 g, 51% yield). R_(f)=0.40 (10% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C18 H25 F N2 O, 304.4, found, 305.4 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) δ 7.10 (t, J=7.8 Hz, 1H), 6.78 (dd, J=1.2, 6.6 Hz, 1H), 6.72 (dd, J=1.7, 11.2 Hz, 1H), 3.4 (s, 2H), 3.48-3.38 (m, 1H), 3.13-3.04 (m, 1H), 2.79 (s, 3H), 2.70 (s, 3H), 2.58-2.50 (m, 2H), 2.37-2.30 (m 4H), 2.22-2.12 (m, 2H), 1.60-1.52 (m, 4H); 100 MHz ¹³C NMR (CDCl₃) δ 174.3, 161.2 (d, J_(C−F)=245.7 Hz), 147.0, 131.4, 123.4 (d, J_(C−F)=15.0 Hz), 121.9, 113.0 (d, J_(C−F)=22.5 Hz), 54.0, 52.5, 36.7, 35.8, 35.5, 33.3, 31.6, 23.5.

EXAMPLE 40 [3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl- methanone

TFA (10.2 ml, 133 mmol) was added to a DCE solution (66 ml) of Example 8, N-{2-fluoro-4-[1-hydroxy-3-(pyrrolidin-1-ylcarbonyl)cyclobutyl]benzyl}-pyrrolidine, (2.3 g, 6.7 mmol) and the mixture was heated to 75° C. for 18 hrs and concentrated down to obtain the TFA salt of intermediate [3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobut-2-enyl]-pyrrolidin-1-yl-methanone. This was redissolved in absolute EtOH (67 ml), Wilkinson's catalyst (308 mg) was then added and the mixture was subjected to hydrogenation at 60° C. using 45 psi H₂. After 1 hr 45 min reaction time, it was concentrated down and the residue was redissolved into 1N HCl (50 ml) and extracted twice with EtOAc (2×120 ml). The aqueous layer was then basified with 15% aq. NaOH (40 ml) and extracted with EtOAc (3×200 ml). The combined organic phases were dried over MgSO₄, and concentrated down to obtain crude material. This was purified by flash chromatography using a 120 g ISCO™ column and 4% MeOH/CH₂Cl₂ with 0.1% NH₄OH. The product containing fractions were combined and concentrated under reduced pressure to give the title compound (1.1 g, 50% yield). R_(f)=0.40 (10% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C20 H27 F N2 O, 330.4, found, 331.4 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) δ 7.30 (t, J=7.6 Hz, 1H), 6.97 (dd, J=1.3, 7.9 Hz, 1H), 6.91 (dd J=1.3, 11.2 Hz, 1H), 3.66 (s, 2H), 3.73-3.63 (m, 1H), 3.50 (t, J=6.7 Hz, 2H), 3.31 (t, J=6.5 Hz, 2H), 3.22-3.14 (m, 1H), 2.77-2.69 (m, 2H), 2.58-2.51 (m, 4H), 2.38-2.28 (m, 2H), 1.97-1.81 (m, 4H), 1.80-1.74 (m, 4H); 100 MHz ¹³C NMR (CDCl₃) δ 173.4, 161.4 (d, J_(C−F)=246.5 Hz), 147.4, 131.6 (d, J_(C−F)=4.50 Hz), 122.1, 113.2 (d, J_(C−F)=22.6 Hz), 112.5, 54.1, 52.7, 46.2, 46.1, 36.1, 34.6, 31.5, 26.3, 24.5, 23.6.

EXAMPLE 41 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid isobutyl-amide

TFA (14 ml, 184 mmol) was added to a DCE solution (80 ml) of Example 20, 3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid isobutyl-amide (3.2 g, 9.2 mmol) and the mixture was heated to 75° C. for 18 hrs and concentrated down to obtain the TFA salt of intermediate 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobut-2-enecarboxylic acid isobutyl-amide. This was redissolved in absolute EtOH (90 ml). Wilkinson's catalyst (424 mg) was then added and the mixture was subjected to hydrogenation at 60° C. using 45 psi H₂. After 2 hr reaction time, it was concentrated down and the residue was redissolved into 1N HCl (60 ml) and extracted twice with EtOAc (2×120 ml). The aqueous layer was then basified with 15% NaOH (40 ml) and extracted with EtOAc (3×250 ml). The combined organic phases were dried over MgSO₄, and concentrated down to obtain crude material. This was purified by flash chromatography using a 120 g ISCO™ column and 4% and 86% MeOH/CH₂Cl₂ with 0.1% NH₄OH. The product containing fractions were combined and concentrated under reduced pressure to give the title compound (885 mg, 26% yield). R_(f)=0.30 (10% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C20 H29 F N2 O, 332.4, found, 333.5 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) δ 7.22 (t, J=7.9Hz, 1H), 6.87 (dd, J=0.8, 8.7 Hz, 1H), 6.82 (dd, J=1.2, 16.2 Hz, 1H), 6.08 (m) 3.72-3.61 (m, 1H), 3.57 (s, 2H), 3.04 (t, J=6.3 Hz, 2H), 3.02-2.82 (m, 1H), 2.65-2.56 (m, 2H), 2.50-2.43 (m, 4H), 2.32-2.22 (m, 2H), 1.78-1.66 (m, 5H), 0.86 (s, 3H), 0.84 (s, 3H); 100 MHz ¹³C NMR (CDCl₃) δ 175.5, 161.4 (d, J_(C−F)=246.4 Hz), 147.1, 131.5, 123.4 (d, J_(C−F)=14.3 Hz) 122.1, 113.1 (d, J_(C−F)=22.5 Hz), 54.1, 52.7, 47.1, 36.6, 36.3, 32.3, 28.8, 23.6, 20.3.

EXAMPLE 42 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethylamide

TFA (13 ml, 169 mmol) was added to a DCE solution (71 ml) of Example 12, 3-(3-Fluoro-4-pyrrolidin-1-ylmethy-phenyl)-3-hydroxy-n-.yrobutiecarboxylic acid ethylamide (2.7 g, 8.4 mmol) and the mixture was heated to 75° C. for 18 hrs and concentrated down to obtain the TFA salt of intermediate 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)cyclabut-2-enecarboxylic acid ethylamide. This was redissolved in absolute EtOH (84 ml). Wilkinson's catalyst (390 mg) was then added and the mixture was subjected to hydrogenation at 60° C. using 45 psi H₂. After 2 hr reaction time, it was concentrated down and the residue was redissolved into 1N HCl (60 ml) and extracted twice with EtOAc (2×120 ml). The aqueous layer was then basified with 15% NaOH (40 ml) and extracted with EtOAc (3×250 ml). The combined organic phases were dried over MgSO₄, and concentrated down to obtain crude material. This was purified by flash chromatography using a 120 g ISCO™ column and 4% and 8% MeOH/CH₂Cl₂ with 0.1% NH₄OH. The product containing fractions were combined and concentrated under reduced pressure to give the title compound (1.0 g, 39% yield). R_(f)=0.30 (10% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C18 H25 F N2 O, 304.4, found, 305.5 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) δ 7.13 (t, J=7.9 Hz, 1H), 6.79 (dd, J=1.2, 7.9 Hz, 1H), 6.82 (dd, J=11.2, 0.8 Hz, 1H), 6.68 (m, 1H), 3.64-3.53 (m, 1H), 3.48 (s, 2H), 3.20-3.10 (m, 2H), 2.94-2.84 (1H), 2.58-2.50 (m, 2H), 2.44-2.32 (m, 4H), 2.22-2.12 (m, 2H), 1.66-1.56 (m, 4H), 1.00 (t, J=24.5 Hz, 3H); 100 MHz ¹³C NMR (CDCl₃) δ 175.4, 161.2 (d, J_(C−F)246.5 Hz), 147.1, 131.4, 123.3 (d, J_(C−F)=15.0 Hz), 121.9, 113.0 (d, J_(C−F)=21.8 Hz), 54.0, 36.5, 36.1, 34.5, 34.2, 32.1, 23.6, 15.0.

EXAMPLE 43 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic, acid ethyl- methyl-amide

TFA (13.5 ml, 17 mmol) was added to a DCE solution (87 ml) of Example 14, 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid ethyl-methyl-amide (2.9 g, 8.74 mmol) and the mixture was heated to 75° C. for 18 hrs and concentrated down to obtain the TFA salt of intermediate 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobut-2-enecarboxylic acid ethyl-methyl-amide. Thts was redissolved in absolute EtOH (87 ml). Wilkinson's catalyst (404 mg) was then added and the mixture was subjected to hydrogenation at 60° C. using 45 psi H₂. After 2 hr reaction time, it was concentrated down and the residue was redissolved into 1N HCl (60 ml) and extracted twice with EtOAc (2×120 ml). The aqueous layer was then basified with 15% NaOH (40 ml) and extracted with EtOAc (3×250 ml). The combined organic phases were dried over MgSO₄, and concentrated down to obtain crude material. This was purified by flash chromatography using a 120 g ISCO™ column and 4% and 8% MeOH/CH₂Cl₂ with 0.1%. NH₄OH. The product containing fractions were combined and concentrated under reduced pressure to give the title compound (1.4 g, 44% yield). R_(f)=0.30 (10% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C19 H27 F N2 O, 318.4, found, 319.5 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) δ 7.19 (t, J=7.9 Hz, 1H), 6.85 (dd, J=1.3, 7.9 Hz, 1H), 6.79 (dd, J=1.3, 11.2 Hz, 1H), 3.58-3.44 (m, 1H), 3.52 (s, 2H), 3.33 (q J=2.9 Hz, 1H), 3.20-3.07 (m, 2H), 2.83 (s, 3H), 2.76 (s, 3H), 2.66-2.56 (m, 2H), 2.45-2.36 (m, 4H), 2.28-2.18 (m, 2H), 1.68-1.58 (m, 4H), 1.02 (q, J=7.1 Hz, 3H); 100 MHz ¹³C NMR (CDCl₃) 1:1 mixture of rotomers, δ 174.2, 173.9, 161.2 (d, J_(C−F)246.5 Hz), 147.1, 131.4, 123.3 (d, J_(C−F)=15.0 Hz), 121.9, 113.1 (d, J_(C−F)=21.8 Hz, 54.0, 52.6, 43.9, 42.6, 36.0, 35.8, 34.1, 33.6, 33.0, 32.7, 31.8, 31.6, 23.6, 13.8, 12.4.

EXAMPLE 44 [3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutyl]-piperidin-1-yl- methanone

A solution of example 32, [3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutyl]-piperidin-1-yl-methanone (106 mg, 0.28 mmol) in dichloromethane (5 ml) was chilled to −78° C. and added drop wise to a −78° C. chilled solution of BAST (0.1 ml, 0.31mmol) in dichloromethane (5 ml) under nitrogen. After 1 hr stirring at −78° C., the reaction mixture was poured into saturated aq. NaHCO₃ (15 ml) and stirred for 15 min. The layers were separated and after two more extractions of the aqueous phase with CH₂Cl₂ (2×20 ml), the combined organic extracts were dried over MgSO₄ and concentrated under reduced pressure to obtain a residual oil. This was purified by flash column chromatography using a 12 g ISCO™ column and 2% MeOH/CH₂Cl₂ to recover the title compound (28 mg, 26% yield). R_(f)=0.30 (5% MeOH/CH₂Cl₂), LRMS m/z Calcd for C21 H28 Cl F N2 l O, 378.9, found, 379.4, 359.4 (M+1) & (M+1−HF) APCl; 400 MHz ¹H NMR (CDCl₃) δ 7.50-7.46 (m, 1H), 7.40 (bs, 1H),

7.28 (dd, J=1.3, 7.9 Hz, 1H), 3.74 (s, 2H), 3.70-3.52 (m, 3H), 3.38-3.32 (m, 2H), 2.96-2.80 (m, 2H), 2.78-2.66 (m, 2H), 2.64-2.54 (m, 4H), 1.84-1.74 (m, 4H), 1.68-1.60 (m, 2H), 1.58-1.50 (m, 4H).

EXAMPLE 46 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid isobutyl-methyl-amide

A solution of Example 33, 3-(3-chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid isobutyl-methyl-amide (102 mg, 0.27 mmol) in dichloromethane (5 ml) was chilled to −78° C. and added drop wise to a −78° C. chilled solution of BAST (0.06 ml, 0.30 mmol) in dichloromethane (5 ml ) under nitrogen. After 1 hr stirring at −78° C., the reaction mixture was poured into saturated aq. NaHCO₃ (15 ml) and stirred for 15 min. The layers were separated and after two more extractions of the aqueous phase with CH₂Cl₂ (2×20 ml), the combined organic extracts were dried over MgSO₄ and concentrated under reduced pressure to obtain a residual oil. This was purified by flash column chromatography using a 12 g ISCO™ column and 2% MeOH/CH₂Cl₂ to recover the title compound (24 mg, 23% yield).

R_(f)=0.35 (5% MeOH/CH₂Cl₂); LRMS m/z Calcd for C21 H30 Cl F N2 O, 380.9, found, 381.4, 361.4 (M+1) & (M+1−HF) APCl; 400 MHz ¹H NMR (CDCl₃) 1:1 mixture of rotomers, diagnostic peaks, δ 3.74 (s, 2H), 3.56-3.51 (m, 1H), 3.21 (d, J=7.9 Hz., 1H), 3.07 (d, J=7.5 Hz, 1H), 2.62-2.54 (m, 4H), 2.00-1.88 (m, 1H), 1.85-1.75 (m, 4H); 100 MHz ¹³C NMR (CDCl₃) 1:1 mixture of rotomers, δ 173.7, 173.4, 136.8, 134.0, 130.8, 125.8, 125.8, 121.3, 123.2, 57.2, 56.8, 55.5, 54.4, 35.6, 34.2, 31.0, 39.2, 27.6, 26.9, 23.8, 20.2, 20.1.

EXAMPLE 46 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid cyclopropylmethyl-amide

A solution of Example 34 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid cyclopropyl methyl-amide (92 mg, 0.25 mmol) in dichlodromethane (5 ml) was chilled to −78° C. and added drop wise to a −78° C. chilled solution of BAST (0.05 ml, 0.28 mmol) in dichloromtethane (5 ml) under nitrogen. After 1 hr stirrinq at −78° C., the reaction mixture was poured into saturated aq. NaHCO₃ (15 ml) and stirred for 15 min. The layers were separated and after two more extractions of the aqueous phase with CH₂Cl₂ (2×20 ml), the combined organic extracts were dried over MgSO₄ and concentrated under reduced pressure to obtain a residual oil. This was purified by flash column chromatography using a 12 g ISCO™ column and 2% MeOH/CH₂Cl₂ to recover the title compound (47 mg, 51% yield). R_(f)=0.35 (5% MeOH/CH₂Cl₂); LRMS m/z Calcd for C20 H26 Cl F N2 O, 364.9, found, 365.4, 345.4 (M+1) & (M+1−HF) APCl; 400 MHz ¹H NMR (CDCl₃) δ 7.48 (dd, J=1.2, 7.9 Hz, 1H), 7.43 (bs, 1H), 7.33 (dd, J=1.3, 7.9 Hz, 1H), 5.69 (bs, 1H), 3.73 (s, 2H), 3.32-3.22 (m, 1H), 3.15-3.10 (2H), 2.95-2.80 (m, 2H), 2.78-2.63 (m, 2H), 2.62-2.52 (m, 4H), 1.85-1.75 (m, 4H), 1.00-0.88 (m, 1H), 0.54-0.46 (m, 2H), 0.22-0.15 (m, 2H); 100 MHz ¹³C NMR (CDCl₃) 1:1 mixture of rotomers, peak list δ 173.6, 142.0, 141.8, 134.0, 130.7, 125.8, 123.3, 56.9, 51.4, 44.8, 38.9, 38.6, 33.4, 23.8, 10.9, 3.6.

EXAMPLE 47 AND EXAMPLE 48 cis 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide and trans 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)- cyclobutanecarboxylic acid methylamide

TFA (34.7 ml, 450 mmol) was added to a DCE solution (150 ml) of example 18, 3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid methylamide (10 g, 31.4 mmol) and the mixture was heated to 75° C. for 18 hrs and concentrated down to obtain the TFA salt of intermediate 3-(3-fluoro-4- (pyrrolidin-1-yl)methyl)phenyl-cyclobut-2-enecarboxylic acid methylamide. This was redissolved in absolute EtOH (79 ml), Wilkinson's catalyst (1000 mg) was then added and the mixture was subjected to hydrogenation at 60° C. using 45 psi H₂. After 2 hr reaction time, it was concentrated down and the residue was redissolved into 1N HCl (100 ml) and extracted twice with EtOAc (2×100 ml). The aqueous layer was then basified with 1N NaOH (100 ml) and extracted with EtOAc (2×50 ml). The combined organic phases were dried over MgSO₄, and concentrated down to obtain crude material. This was purified by flash chromatography using a 120 g ISCO™ column and 5%, 10% and 15% MeOH/CH₂Cl₂ with 0.25% NH₄OH. The product containing fractions were combined and concentrated under reduced pressure to give a mixture of cis, trans isomers (4.8 g, 74% yield). The isomers were separated by preparative chromatography on a Chiralcel OD (10 cm ×50 cm) column at a flow-rate of 295 ml/min and using Heptane/EtOH (95/5) as eluent to recover trans 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide (2.5 g) and cis 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)cyclobutanecarboxylic acid methylamide (0.23 g).

EXAMPLE 47

cis 3-(3Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide. R_(f)=0.50 (25% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C17 H23 F N2 O, 290.4, found, 291.1 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) δ 7.19 (t, J=7.8Hz, 1H), 6.89 (dd, J=1.3, 8.2 Hz, 1H), 6.83 (dd, J=1.3, 10.9 Hz, 1H), 3.54 (s, 2H), 3.30-3.19 (m, 1H), 2.94-2.84 (m, 1H), 2.68 (d, J=4.7 Hz, 3H), 2.48-2.36 (m, 6H), 2.34-2.24 (m, 2H), 1.72-1.64 (m, 4H); 100 MHz ¹³C NMR (CDCl₃) δ 175.1, 161.2 (d, J_(C−F)=245.5 Hz), 146.4, 146.2, 131.4, 122.1 (d, J_(C−F)=15.0 Hz), 113.4 (d, J_(C−F)=22.8 Hz), 54.0, 52.7, 35.7, 35.4, 32.9, 26.4, 23.5.

EXAMPLE 48

trans 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide: R_(f)=0.50 (25% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C17 H23 F N2 O, 290.4, found, 291.1 (M+1) APCl; 400 MHz ¹H NMR (CDCl₃) δ 7.16 (t, J=7.8 Hz, 1H), 6.85-6.74 (m, 2H), 4.03 (bs, 1H), 3.62-3.50 (m, 1H), 3.55 (s, 2H), 3.25 (s, 3H), 2.68-2.40 (m, 7H), 2.24-2.13 (m, 2H), 1.70-1.62 (m, 4H); 100 MHz ¹³C NMR (CDCl₃) δ 176.7, 161.2 (d, J_(C−F)=245.5 Hz), 147.7, 147.5, 131.8, 122.1 (d, J_(C−F)=15.0 Hz), 113.2 (d, J_(C−F)=22.8 Hz), 53.7, 52.3, 50.0, 35.6, 32.0, 26.4, 23.3.

EXAMPLE 49 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid methyl-tetrahydro-pyran-4-ylmethyl)-amide

A solution of Example 35, 3-(3-Chloro-4-pyrroldin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid methyl-(tetrahydro-pyran-4-ylmethyl)-amide (70 mg, 0.17 mmol) in dichloromethane (5 mL) was chilled to −78° C. and added drop wise to a −78° C. chilled solution of BAST (0.034 ml, 0.18 mmol) in dichloromethane (5 ml) under nitrogen. After 1 hr stirring at −78° C., the reaction mixture was poured into saturated aq. NaHCO₃ (15 ml) and stirred for 15 min. The layers were separated and after two more extractions of the aqueous phase with CH₂Cl₂ (2×20 ml), the combined organic extracts were dried over MgSO₄ and concentrated under reduced pressure to obtain a residual oil. This was purified by flash column chromatography using a 12 g ISCO™ column and 2% MeOH/CH₂Cl₂ to recover a mixture of cis/trans isomers of the title compound (40 mg, 57% yield).

R_(f)=0.30 (10% MeOH/CH₂Cl₂); LRMS m/z Calcd for C23 H32 Cl F N2 O2, 422.9, found, 423.4 (M+1) & 403.4 (M+1−HF).

EXAMPLE 50 3-(3-Choro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid cyclopropylmethyl-methyl-amide

A solution of Example 36, 3-(3-chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarbolxylic acid cyclopropylmethyl-methyl-amide (75 mg, 0.20 mmol) in dichloromethane (5 ml) was chilled to −78° C. and added drop wise to a −78° C. chilled solution of BAST (0.074 ml, 0.4 mmol) in dichloromethane (5 ml) under nitrogen. After 1 hr stirring at −78° C., the reaction mixture was poured into saturated aq. NaHCO₃ (15 ml) and stirred for 15 min. The layers were separated and after two more extractions of the aqueous phase with CH₂Cl₂ (2×20 ml), the combined organic extracts were dried over MgSO₄ and concentrated under reduced pressure to obtain a residual oil. This was purified by flash column chromatography using a 12 g ISCO™ column and 2% MeOH/CH₂Cl₂ to recover a mixture of cis/trans isomers of the title compound (75 mg, 99% yield). R_(f)=0.60 (15% MeOH/CH₂Cl₂); LRMS m/z Calcd for C21 H28 Cl F N2 O, 378.9, found, 379.4 (M+1) & 359.4 (M+1−HF)

EXAMPLE 51 [3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutyl]-(2,3-dihydro- 5H-benzo[f][1,4]oxazepin-4-yl)-methanone

A solution of Example 37, [3-(3-chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutyl]-(2,3-dihydro-5H-benzo[f][1,4]oxazepin-4-yl)-methanone (82 mg, 0.19 mmol) in dichloromethane (5 ml) was chilled to −78° C. and added drop wise to a −78° C. chilled solution of BAST (0.069 ml, 0.37 mmol) in dichloromethane (5 ml) under nitrogen. After 1 hr stirring at −78° C., the reaction mixture was poured into saturated aq. NaHCO₃ (15 ml) and stirred for 15 min. The layers were separated and after two more extractions of the aqueous phase with CH₂Cl₂ (2×20 ml), the combined organic extracts were dried over MgSO₄ and concentrated under reduced pressure to obtain a residual oil. This was purified by flash column chromatography using a 12 g ISCO™ column and 204 MeOH/CH₂Cl₂ to recover a mixture of cis/trans isomers of the title compound (80 mg, 99% yield). R_(f)=0.65 (15% MeOH/CH₂Cl₂); LRMS m/z Calcd for C25 H28 Cl F N2 O2, 442.9, found, 443.9 (M+1) & 423.9 (M+1−HF)

EXAMPLE 52 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid methyl-(3-methyl-pyridin-2-ylmethyl)-amide

A solution of example 38, 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl-3-hydroxy-cyclobutanecarboxylic acid methyl-(3-methyl-pyridin-2-ylmethyl)amide (116 mg, 0.27 mmol) in dichloromethane (5 ml) was chilled to −78° C. and added drop wise to a −78° C. chilled solution of BAST (0.1 ml, 0.54 mmol) in dichloromethane (5 ml) under nitrogen. After 1 hr stirring at −78° C., the reaction mixture was poured into saturated aq. NaHCO₃ (15 ml) and stirred for 15 min. The layers were separated and after two more extractions of the aqueous phase with CH₂Cl ₂ (2×20 ml), the combined organic extracts were dried over MgSO₄ and concentrated under reduced pressure to obtain a residual oil. This was purified by flash column chromatography using a 12 g ISCO™ column and 2% MeOH/CH₂Cl₂ to recover the title compound (88 mg, 76% yield).

R_(f)=0.45 (15% MeOH/CH₂Cl₂); LRMS m/z Calcd for C₂₄H₂₈ClFN₃O, 429.96, found, 430.4 (M+H) APCl; representative ¹H-NMR peaks: (CDCl₃) δ 4.70 (s, 2H), 3.75 (s, 2H), 1.78 (s, 4H).

Intermediate 18a

4-Bromo-2,6-fluorobenzaldehyde

n-BuLi (2.7 M solution in heptane, 134 mL, 0.36 mol) was added drop wise at −75° C. to a solution of (i-Pr)₂NH (51 ml, 0.36 mol) in THF (300 mL) and the mixture was stirred at the same temperature for 5 min. 1-Bromo-3,5-difluorobenzene (CAS 461-91-1) (70 g, 0.36 mol) in THF (100 ml) was added to the mixture at −80° C. and the mixture was stirred at the same temperature for 2h. DMF (28 mL, 0.36 mol) was added to the mixture at −80° C., and the mixture was stirred at the same temperature for 15 min. A solution of AcOH in Et₂O (1:1, 100 ml) was added to attain pH˜45 at −80° C., and the reaction mixture was stirred at RT for 15 min. Water (500 mL) was added, and the layers were separated. The aqueous layer was extracted Et₂O (300 mL). The combined organic phases were washed with water, brine, dried with anhydrous Na₂SO₄ (100 g), evaporated and recrystallized from hexane to give the title compound (53.5 g, 67%, 0.24 mol) as white crystals. GC/MS data: 219 and 221 (M−H)⁺; 220 and 222 (M)+ (calculated for C₇H₃BrF₂O 221). 1H NMR data (DMSO-6) δ 10.15 (s, 1H, CHO), 7.71-7.65 (m, 2H, Ar—H).

Intermediate 18

1-(4-Bromo-2,6difluorobenzyl)pyrrolidine

Pyrrolidine (25 mL, 0.30 mol) and sodium triacetoxyborohydride (64 g, 0.30 mol) were added in portions to a stirred solution of Intermediate 18a, 4-Bromo-2,6-difluorobenzaldehyde (53.5 g, 0.24 mol) in dichloromethane (500 mL) on ice bath. The reaction mixture was intensively stirred for 12 h at RT. Water (400 mL) was added followed by addition of 5 M aq. NaHSO₄ to attain pH˜2. The organic layer was separated. The aqueous one was extracted with CH₂Cl₂ (2×200 mL). The organic layers were discarded. The aqueous fraction was alkalized with K₂CO₅ to pH˜1o, and extracted with CHCl₃ (2×300 mL). The organic extract was washed with brine, dried over anhydrous Na₂SO₄ (100 g) and evaporated in vacuo to give the title compound (52.5 g, 79%, 0.19 mol). LC/MS data: 275.9 and 277.9 (M+H)⁺ (calculated for C₁₁H₁₂BrF₂N, 276.13). 1H NMR data (DMSO-d6): 7.40-7.48 (m, 2H Ar—H), 3.66 (s, 2H, Ar—CH₂), 2.38-2.46 (m, 4H pyrrolidine (CH₂)₂N), 1.61-1.71 (m, 4H, CH₂CH₂CH₂CH₂).

EXAMPLE 53 [3-(3,5-Difluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hyroxy-cyclobutyl]- pyrrolidin-1-yl-methanone

A 2.5 M solution of n-BuLi in hexanes (4.34 ml, 10.9 mmol) was added over 15 min to a solution of intermediate 18, 1-(4-bromo-2,6-difluoro-benzyl)-pyrrolidine (3.0 g, 10.9 mmol) in absolute THF (20 ml) under a flow of nitrogen at −78° C. The reaction mixture was stirred at −78° C. for 30 min. Then a −78° C. chilled solution of 3-oxocyclobutanecarboxylic acid (0.62 g, 5.43 mmol) in absolute THF (6 ml) was added drop wise at −78° C. The mixture was warmed to RT slowly and left stirring for 18 hrs. Pyrrolidine (0.674 ml, 8.15 mmol) and T ₃P (3.8 ml, 5.97 mmol, 50% solution in EtOAc) were added and stirred for 30 min and the reaction was then quenched with 1N NaOH (25 ml) and extracted with CH₂Cl₂ (3×100 ml) to recover 2.8 g of crude product. This was purified by flash column chromatography using a 120 g ISCO™ column and 5% and 8% MeOH/CH₂Cl₂ with 0.2% NH₄OH to obtain the title compound (520 mg, 26% yield). R_(f)=0.75 (20% MeOH/CH₂Cl₂+0.2% NH₄OH); LRMS m/z Calcd for C₂₀H₂₆F₂N₂O₂, 364.2, found, 365.4 (M+H) APCl; ¹H-NMR (CDCl₃)δ 7.03 (ddd, J=8.7, 3.7 2.5 Hz, 2H), 3.76 (s, 2H), 3.51 (t, J=6.8 Hz, 2H), 3.47-3.40 (m, 4H), 3.13-3.06 (m, 1H), 2.80-2.73 (m, 2H), 2.60-2.50 (m, 5H), 2.00-1.90 (m, 2H), 1.90-1.83 (m, 2H), 1.76-1.72 (m, 4H).

EXAMPLE 54 [3-(3,5-Difluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutyl]-pyrrolidin- 1-yl-methanone

A solution of Example 53, [3-(3,5-Difluoro-4-pyrrolidin-1-ethyl-phenyl)-3-hydroxy-cyclobutyl]-pyrroldin-1-yl-methanone (250 mg, 0.68 mmol) in dichloromethane (5 ml) was chilled to −78° C. and added drop wise to a −78° C. chilled solution of BAST (0.25 ml, 1.37 mmol) in dichloromethane (5 ml) under nitrogen. After 1 hr stirring at −78° C., the reaction mixture was poured into saturated aq. NaHCO₃ (20 ml) and stirred for 15 min. The layers were separated and after two more extractions of the aqueous phase with CH₂Cl₂(2×50 ml), the combined organic extracts were dried over MgSO₄ and concentrated under reduced pressure to obtain a residual oil. This was purified by flash column chromatography using a 12 g ISCO™ column and 2% MeOH/CH₂Cl₂ to give the title compound (130 mg, 52% yield). R_(f)=0.50 (15% MeOH/CH₂Cl₂); LRMS m/z Calcd for C₂₀H₂₅F₃N₂O, 366.2, found, 367.4 (M+H) APCl; ¹H-NMR (CDCl₃) δ 6.94 (ddd, J=7.9, 4.6, 2.5 Hz, 2H), 3.68 (s, 2H), 3.51-3.25 (m, 5H), 2.84-2.59 (m, 4H), 2.47 (br s, 4H), 1.93-1.84 (m, 2H), 1.83-1.75 (m, 2H), 1.69-1.54 (m, 4H); ¹³C-NMR (CDCl₃) δ 171.2, 161.9 (dd, J_(C−F)=248.7, 9.0 Hz), 144.4-143.9 (multiplet), 113.7-113.4 (multiplet), 107.8 (dd, J_(C−F)=27.8, 8.7 Hz), 96.7 (d, J_(C−F)=196.1 Hz), 53.4, 46.3, 46.1, 38.4 (d, J_(C−F)=24.8 Hz), 31.2, 26.2, 24.4, 23.6.

EXAMPLE 20—ALTERNATIVE PREPARATION 3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid isobutyl-amide

A 2.5 M solution of n-BuLi in hexanes (155 ml, 388 mmol) was added over 30 min to a solution of 1-(4-bromo-2-fluoro-benzyl)-pyrrolidine (100 g, 388 mmol) in THF (600 ml) in a 2 L round bottomed flask under a flow of nitrogen at −78° C. The reaction mixture was stirred at −78° C. for 60 min. Then a −78° C. chilled solution of 3-oxocyclobutanecarboxylic acid (22 g, 194 mmol) in THF (264 ml) was cannulated under nitrogen and at −78° C. The mixture was warmed to RT slowly and left stirring for 18 h. Isobutylamine (38.5 mL 388 mmol) and T₃P (148 ml, 233 mmol, 50 wt % solution in EtOAc) were added. The mixture was stirred for 60 min and then quenched with 1N NaOH (800 ml) and diluted with another 800 ml of EtOAc. The layers were separated and the aqueous phase was extracted with EtOAc (2×1 L) to recover crude product. This was purified by flash column chromatography using a 75 L Biotage™ columnn elutirng with 100% EtOAc, followed with 25%, 30%, and 40% MeOH/EtOAc. The fractions containing the product were combined and concentrated under reduced pressure to obtain a semi solid which was triturated in Et₂O and filtered to obtain the title compound as a white solid (43 g, 61% yield).

EXAMPLE 55 3-Fluoro-3-(3-fiuoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid isobutyl-amide

A slurry of Example 20, 3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-l3-hydroxy-cyclobutanecarboxylic acid isobutyl-amide (32.0 g, 91.8 mmol) in anhydrous THF (1.2 L') was chilled to −78° C. and cannulated into a 2 L round bottomed flask containing a −78° C. chilled solution of BAST (33.8 ml, 183.5 mmol) in anhydrous THr (500 mL) under nitrogen. The resulting reaction slurry was slowly warmed to RT and left stirring for 18 hrs evhen it turned clear. The reaction mixture was poured into saturated aq. NaHC0₃ (1 L) and diluted with 1.5 L of EtOAc and stirred for 30 mmn. 7The layers were separated and after two more extractions of the aqueous phase With EtOAc (2×1 L), the combined organic extracts were dried over MgSO₄ and concentrated tinder reduced pressure to obtain a residual oil (35 g). This was purified by flash column chrominatography using a 75 L Biotage™ column and CH₂Cl₂, 5%/ and 10% MeOH/CH₂Cl₂ to recover a cis:trans mixture of the title compound (32 g, 91% yield). Cis:trans isomers were separated using Chirapak™ AS column (10 cm×50 cm) and 90/10 Heptane/IPA with 0.2% diethylamine azs an eluent and at a fow rate of 450 ml/min to obtain the title compound (27 g. 85% yield): R_(f)=0.25 (10% MeOH/CH₂CHCl₂). LRMS mlz Calcd for C₂₀H₂₈F₂N₂O, 350.2, found, 351.4 (M+H) & 331.4 (,M+H−HF) APCI; ¹H-NMR (CDCl₃) δ 7.35 (t, J=7.7 Hz, 1H), 7.18 (d, J=7.9 Hz, 1H), 7.11 (d. J=10.8 Hz, 1H) 5.96 (brs, 1H), 3.63 (s, 2H), 3.27 (p, J=8.5 Hz, 1H), 3.05 (t, J=6.4 Hz, 2H), 2.90-2.77 (m, 2H), 2.72-2.61 (m, 2H), 2.49 (br s, 4H), 1.80-1.67 (m, 5H), 0.86 (d, J=6.6 Hz, 6H); ¹³C-NMR (CDCl₃) δ 173.9, 161.1, (d, J_(C-F)=246.5 Hz), 143.0 (dd, J^(C-F)=24.1, 7.1 Hz), 131.5 (d, J=4.5 Hz), 125.9 (d, J_(C-F)=14.3 Hz), 120.3 (dd. J_(C-F)=7.5, 3.0 Hz), 111.9 (dd, J_(C-F)=24.0, 9.0 Hz), 97.2 (d, J_(C-F)=193.9 Hz), 54.1, 52.7, 47 22, 38 8 (d, J_(C-F)=25.6 Hz, 33.24, 28.7, 23.6, 20 3.

EXAMPLE 9 (ALTERNATIVE PREPARATION) [3-Fluoro-3-(3-fuoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl-methanone EXAMPLE 56 [3-Fluoro-3-(3-fuoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl-methanone

A solution of Example 8, [3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenlyl)-3-hydroxy-cyclobutyl]-pyrrolidin-1-yl-methanone (1.2 g, 3.5 mmol) in dichloroomethane (10 ml) was chilled to −78° C. and added drop wise to a −7 8° C. chilled solution of BAST (0.96 ml, 5.2 mmol) in dichlorornethane (7.5 ml) under nitrogen. After lhrstirmng at −78° C., the reaction miuxttre was poured into saturated aq. NaHC0₃ (50 mL) and stirred for 15 min. The layers were separated and after two more extractions of the aqueous phase witth CH₂Cl₂ (2×75 ml), the combined organic extracts were dried over MGSO₄ and concentrated under reduced pressure to obtain a residual oil. This vtas punfied by flash column chro,matography using a 220 g ISCO™ column and 20% MeOH/EtOAc to recover a mixture of cisttrans isomers of the title compound (660 nmg, 54% yield). This was purnfied using chromatography on a Chiralcel™ OJ (2.1 cm×25 cm) column using 95/5 Heptane/EtOH with 0.1% DEA as an eluent at a flow rate of 20 ml/min to obtain 370 mg of the Example 9 and 45 mg of Example 56.

EXAMPLE 56

[3-Fluoro-(3-(-fuoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl-methanone: R_(f)=0.30 (20% MeOH/EtOAc); LRMS m/z Calod for C₂₉H₂₆F₂N₂O, 348.2, found, 349.4 (M+H) & 329.4 (M+H−HF) APCI, ¹H-NMR (CDCl₃) δ 7.43 (t, J=7.7 Hz, 1H) 7.20 (d, J=7.9 Hz, 1H), 7.13 (d, J=10.8 Hz, 1H), 3.68 (s, 2H), 3.46 (t, J=6.8 Hz, 2H) 3.32 (t, J=6.8 Hz, 2H), 3.04-2.93 (m, 2H), 2.79-2.65 (m, 3H), 2.55 (br s, 4H), 1.95-1.88 (m, 2H), 1.86-1.80 (m, 2H), 1.79-1.72 (m, 4H), ¹³C-NMR (CDCl₃) δ 171.2, 161.26 (d, J_(C-F)=2472 Hz), 143.1-142.6 (multpllet), 131.8 (d, J_(C-F)=4.5 Hz), 126.06, 120.4. 112.2 (dd, J_(C-F)=24.1, 6.8 Hz), 91.6 (d, J_(C-F)=159.3 Hz). 54.19, 52.61, 46.2. 38.5, 38.3, 28.2 (d, J_(C-F)=13.5 Hz), 26.2, 24.4, 23.7.

INTERMEDIATE 19 (4-Bromo-2-chloro-phenyl)-pyrrolidin-1-yl-methanone

4-Bromo-2-chloro-benzoic acid (30g, 127.4 mmol) was placed in a 3 L round bottom flask and 1.5 L of EtOAc was transferred into it. Triethylamine (25.8 g, 255 mmol), pyrrolidine (18 g, 255 mrnol), and T₃P (48.6 g, 152.9 mmol, 50 wt % in EtOAc) were then added, After 1 hr, the reaction was quenched with 200 mL of 1N NaOH and stirred for 10 min. The layers were separated and after 2 more extractions of the aqueous phase with EtOAc (2×500 ml), the combined organic extracts were dried over MgSO₄ and fitered. The filtrate was concentrated under reduced pressure to obtain a viscous oil. Flash chromatography using a 330 g ISCO™ column and 50%, 80% EtOAc/Hexanes yielded the title compound as a light yellow colored viscous oil (35.4 g. 96 % yield) R_(f)=0.25 (50% EtOAc/Hexanes), LRMS m/z Cald for C₁₁H₁₁BrClNO, 288.6, found, 289.9 (M+H) APIC, 1H-NMR (CDOCl₃) & 7.56 (d, J=1.7 Hz, 1H), 7.43 (dd, J=1.7, 8.3 Hz. 1H) 7.17 (d, J=8.3 Hz, 1H), 3.63 (b aptt, J=6.6 Hz, 2H), 3.17 (B apt t, J=6.6 Hz, 2H), 2.00-1.84 (m, 4H). ¹³C-NMR (CDCl₃) δ 166.0, 13.6, 132.6, 131.3, 130.7, 128.9, 123.3, 48.0, 45.8, 261, 24.7.

INTERMEDIATE 20 1-(4-bromo-2-chlorobenzyl) pyrrolidine

To a dry THF solution (120 ml) of 4-Bromo-2-chloro-phenyl)-pyrrojidin-1-yl-methanone (35.3g, 122.3 nmoml) was added drop wise 1.0M BH₃/THF (367 ml, 376 mmcol) under nitrogen and the resulted reaction mixture was left stirring at rt for 21 hrs. The reaction w,vas quenched with 120 ml of MeOH and heated to 80° C. for 18 hrs. It was then cooled to rt and concentrated under reduced pressure to obtain a residual which was purified by flash chromatography using a 330 g ISCO™ column and 50% EtOAc/hexanes to recover the title compound as a colorless viscous oil (24.4 g, 74% yield). Rf=0.25 (60% EtOAc/Hexanes), LPMS m/z Calcd for C₁₁H₁₃BrClN, 274.6, found, 276.0 (M+H) APCI; ¹H-NMR (CDCl₃) δ 7.47 (bs, 1H), 7.38-7.33 (m, 2H), 3.66 (s, 2H), 2.58-2.54 (m, 4H), 1.80-1.76 (m, 4H); ¹³C-NMR (CDCl₃) δ 136.4, 134.8, 132.0, 131.9, 130.0, 120.7, 56.6, 54.4, 23.8.

INTERMEDIATE 17 3-(3-chloro-4-((pyrrolidin-1-yl)methyl)phenyl)-3-hydroxycyclobutanecarboxylic acid

A 2.5 M solution of n-BuLi in hexanes (60 ml, 150 mmol) was added over 15 min to a solution of intermediate 20, 1-(4-bromo-2chlorobenzyl) pyrrolidine (41.2 g, 150 mmol) in THF (350 ml) under a flow of nitrogen at −78° C. The reaction mixture was stirred at −78° C. for 30 min. Then a −78° C., chilled soltution of 3-oxocyclobutanecarboxylic acid (8.6 g, 75 mmol) in THF (100 ml) was added drop wise for 10 min at −78° C. The mixture was warnmed to RT slowly Aand left stirring for 18hrs and the resulting solution w,as used as an internediate.

EXAMPLE 57 3-(3-Chloro-4-pyrrolidin-l-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid ethylamide

To a crude solution of intermediate 17, 3-j3-chioro-4-((pyrrolidin-1-yl)methyl)phenyl)-3-hydroxycyclobutanecarboxyiic acid (−30 mL, −34 mmol) was added 2.0M ethylarmine in THF (34.5 mL, 69 mnmol) and T₃P (50% solution in EtOAc, 33 ml, 51.8 mnmol). The resulting reaction mixture was stirred at PT for 1 hr and then 300 ml of 1N NaOH and 400 ml of EtOAc were added and the layers wvere separated. The aqueous layer was subjected to EtOAc extraction (2×500 ml) again and the combined organic layers wvere dried over MgSO₄ and evaporated. The residue was purified by flash column chromatography using a 75M Biotage™ column, eluting with a gradient of 5%, 8%, 10%, 15% MeOH/CH₂Cl₂ with 0.25% NH₄OH, The product containing fractions were colected and concentrated under reduced pressure to give the title compound (4.0 g, 35% yield). Rf=0.40 (15% MeOH/CH₂Cl₂+0.2% NH₄OH), LRMS m/z Calcd for C₁₅H₂₅ClN₂O₂, 336.9, found. 337.4 (M+H) APCI; ¹H-NMR (CCDl₃) δ 7.54 (dd, J=8.3, 2.7 Hz, 1H), 7.49-7.45 (m, 1H), 7.33 (d, J=7.9 Hz, 1H), 6.52-6.35 (br m, 1H), 3.86 (s, 2H), 3.29-3.22 (m, 2H), 2.81-2.60 (m, 7H), 2.48 (d, J=8.3 Hz, 2H), 1.83 (br s, 4H), 1.11 (dt, J=7.3, 2.9 Hz, 3H) ¹³C-NMR (CDCl₃) δ 176.7, 147.0, 134.2, 133.3, 131.4, 126.5, 123,8, 73.8, 73.9, 56.1, 54.1, 41.2, 35.0, 33.0, 23.6, 14.9.

EXAMPLE 58 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid ethylamide

A solution of Example 57, 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid ethylamide (52.2 g. 155 mmol) in dichloromethane (400 ml) wvas chilled to −78° C. and added drop Rase to a −78° C. solution of BAST (43 ml, 233 mmol) in dichloromethane (150 tml) under nitrogen. This wias slaowvy warmed to rt and left stirring for 18 hrs after which the reaction mixture was poured into saturated aq, NaHC0₃ (1 L) and stirred for 15 min. The layers were separated and after two more extractions of the aqueous phase with CH₂Cl₂ (2×1 L), the combined organic extracts were dried over MgSO₄ and concentrated under reduced pressure to obtain a residual oil. This was purified by flash column chromatography using a 75 L BiotageBiotage™ column and 5%, 10%, 20% MeOH/EtOAc to recover a mixture of cis/trans isomers of the title compound. This was further purified by chromatography using a Chiralcel™ OD (10 cm×50 cm) column with 93/7 Heptane/IPA as an eluent at a flow rate of 435 ml/min to obtain the title compound (31.7 g, 60% yield). Rf=0.30. (15% MeOH/EtOAc), LRMS m/z Calcd for C₁₈H₂₄ClFN₂O, 338.9, found, 339.4 (M+H) APCI; ¹H-NMR (CDCl₃) δ 7.46 (d, J=7.9 Hz, 1H), 7.42 9s, 1H), 7.32 (dd, J=7.1, 1.5 Hz, 1H), 5.76 (br s, 1H), 3.71 (s, 2H), 3.32-3.20 (m, 3H), 2.92-2.77 (m, 2H), 2.73-2.62 (m, 2H), 2.57-2.52 (m, 4H), 1.80-1.73 (m, 4H), 1.12 (t, J=7.3 Hz, 3H); ¹³C-NMR (CDCl₃) δ 173.7, 141.8 (d J_(C-F)=23.3 Hz), 137.0. 134.0. 130.7, 125.8 (d, J_(C-F)=9.0 Hz), 123.2 (d, J_(C-F)=7.5 Hz), 97.2 (d, J_(C-F)=194.6 Hz), 56.9, 54.4, 38.7 (d, J_(C-F)=24.8 Hz). 34.8. 33.4, 23.8 15.1. The structure was confirmed by x-ray crystallography and determined to be (1S,3R)-N-ethyl-3-fluoro-3-(3-fuoro-4-(((S)-2-mnethylpyrrolidin-1-yl)methyl)phenyl)cyclobutanecarboxamnide.

INTERMEDIATE 21 trans-3-[4-(chloromethyl)-3-fluorophenyl]-N-ethvyl-3-fluorocyclobutanecarboxamnide

Ethylchloroformate (0.505 ml, 5.28 mmol) was added to a solution of Example 16, 3-fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethylamide (1.7 g, 5.28 mmol) in DCE (50 ml) After lhr stirring at rt. the reaction was quenched with saturated aq. NaHCO₃ (150 ml) and extracted with CH₂Cl₂ (3×100 ml) to recover a residual oil. This wNas purified by flash column chromatography using a 120 g ISCO™ cartridge and 35% and 40% EtOAc/hexanes to obtain an intermediate 3-(4-chlorornethyl-3-fluoro-phenyl)-3-fluoro-cyclobutanecarboxylic acid ethylamide (1.1 g, 75% yield).

Rf=0.50, (EtOAc/hexanes), LRMS m/z Calcd for C₁₄H₁₆ClF₂NO, 287.7, found, 288.3 (M+H) APCI; ¹H-NMR (CDCl₃) δ 7.30 (t, J=7.9 Hz, 1H), 7.18 (d, 1H), 7.12 (dd, J - 10.8, 1.2 Hz, 1H), 6.85 (br s, 1H), 4.49 (s, 2H), 3.35-3.26 (m, 1H), 3.25-3.15 (m, 2H), 2.85-2.70 m, 2Hl, 2.68-2.52 (m, 2H), 1.03 (t, J=10.9 Hz, 3H), ¹³C-NMR (CDCl₃) δ 173.9, 160.2 (d, J_(C-F)=241.2 Hz), 145.2 (dd, J_(C-F)=24.0, 7.8 Hz), 131.1, 124.5, 120.9, 112.4, (dd, J_(C-F)=23.3, 9.4 Hz, 96.2 (d, J_(C-F)=196.0 Hz), 60 6, 38.8, 34.7, 32.8, 14.8.

EXAMPLE 59 3-Fluoro-3-[3-fuoro-4-((S)-2-methyl-pyrrolidin-1-ylmethyl)-phenyl]-cyclobutanecarboxylic acid ethylamide

To Intermediate 21, trans-3-[4-(chloromethyl)-3-fluorophenyl]-N-ethyl-3-fluorocyclobutanecarboxamide (0.482 g, 1.67 mmol) in DCE (16 ml) was added triethylamine (0.69 ml, 5.01 mmol) and 2-S-methyl pyrrolidine hydrobrornide (0.56 g, 3.35 mmol), This mixture was heated to 50° C. for 3 hrs. The reaction was cooled to d and quenched with saturated aq. NaHCO3 (200 ml) and extracted with CH₂Cl₂ (3×200 ml) to recover 600 mg of crude matrerial. This was purified by flash column chromatography using a 40 g ISCO™ column and 5% and 10% MeOH/EtOAc to obtain the title comnpound (400 mg, 71% yield). Rf=0.50 (15% MeOH/EtOAc); LRMS m/z Calcd for C₁₉H₂₆F₂N₂O, 336.4, found, 337.2 (M+H) APCI; ¹H-NMR (CDCl₃) δ 7.29 (t, J=7.7 Hz, 1H), 7.13 (d, J=7.9 Hz, 1H), 7.06 (d, J=10.8 Hz, 1H), 6.46 (br s, 1H), 3.86 (d. J=13.3 Hz, 1H), 3.29-3.15 (m, 4H), 2.86-2.71 (m, 3H), 2.66-2.54 (m, 2H), 2.36-2.27 (m, 1H), 2.06 (q, J=8.9 Hz, 1H), 1.87-1.78 (m, 1H), 1.67-1.40 (m, 2H), 1.39-1.29(m, 1H), 1.09-1.03 (m, 6H); ¹³C-NMR (CDCl₃) δ 173.9. 161.2 (d, J_(C-F)=246.5 Hz), 142.8 (dd, J_(C-F)=24.0, 7.5 Hz), 131.7 (d, J_(C-F)=4.5 Hz), 126.0 (d, J_(C-F)=15.0 Hz), 120.2 (dd, J_(C-F)=7.5, 3.0 Hz ) 111.8 (dd, J_(C-F)=24.0, 9.0 Hz) 97.1 (d, J_(C-F)=193.9 Hz), 59.4, 54.0, 50.2, 38.6 (dd, J_(C-F)=24.9, 6.4 Hz), 34.7, 33.1, 32.9, 21.7, 19.3, 14.9.

EXAMPLE 60 3-Fluoro-3-[3-fluoro-4-((R)-2-mnethyl-pyrrolidin-1-ylmethyl)-phenyl]-cyclobutanecarboxylic-acid etlylamide

To intermediate 21, trans-3-[4-(chloromethyl -3-fluorophenyl]-N-ethyl-3-fluorocyclobutanecarboxamide (0.48 g, 1.67 mmol) in DCE (16 ml) was added triethylamine (0.69 ml, 5.01 mmol) and 2-R-mnethyl pyrrolidine hydrobromide (0.56 g, 3.35 mmol). This mixture was heated to 50° C. for 3 hrs. The reaction w',as cooled to rt and quenched with saturated aq. NaHOO₃ (200 ml) and extracted with CH₂Cl₂ (3×200 ml) to recover 610 mg of crude material. This ewas purified by ffash colu-imn chromatography using a 40 g ISCO™ column and 5% and 10% MeOH/EtOAc to obtain the title compound (406 mg, 72% yield): Rf=0.50 (15 % MeOH/EtOAc); LRMS m/z Calcd for C₁₉H₂₆F₂N₂O, 336.4, found, 337.2 (M+H), APCI; ¹H-NMR (CDCl₃) δ 7.29 (t, J=7.7Hz, 1H), 7.12 (d, J=7.9 Hz, 1H), 7.05 (dd, J=10.8, 1.3 Hz, 1H), 6.49 (br t, J=5.0 Hz, 1H), 3.86 (d, J=13.2 Hz, 1H), 3.48-3.15 (m, 4H), 2.86-2.71 (m, 3H), 2.65-2.54 (m, 2H), 2.36-2.27 (m, 1H), 2.05 (q, J=8.7 Hz, 1H), 1.86-1.78 (m, 1H), 1.67-1.40 (m, 2H), 1.38-1.27 (m, 1H), 1.10-1.00 (m, 6H); ¹³C-NMR (CDCl₃) δ 173 9, 161.2 (d, J_(C-F)=246.5 Hz), 142.8 (dd, J_(C-F)=23.3, 7.5 Hz), 131.7 (d, J_(C-F)=4.5 Hz), 126.0 (d, J_(C-F)=15.0 Hz), 120.2 (dd, J_(C-F)=7.5. 3 0 Hz ), 111.8 (dd, J_(C-F)=24.0, 9.0 Hz) 97.1 (d, J_(C--F)=194.6 Hz), 59.4, 54.0, 50.2, 38.6 (dd. J_(C-F)=25.6, 6.4 Hz), 34.7, 33.0, 32.9, 21.7, 19.3, 14.9.

EXAMPLE 61 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid dimnethylamide

To a stirring solution of BAST (0.072 g, 0.327 mmol) at −78° C. in dry CH₂Cl₂ (2 ml) was added a solution of Example 31, 3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-hydroxy-cyclobutanecarboxylic acid dimethylamide (0.1 g, 0.296 mmol) in dry CH₂Cl₂ (5 ml) drop wise. After 1 hr, the reaction was quenched cold with sazt. aq. NaHCO₃ (10 ml) and diluted with CH₂Cl₂. The layers were separated and the aqueous phase was once again extracted with CH₂Cl₂ (25 ml) and the combined organic phases were dried over MgSO₄ and concentrated under reduced pressure to obtain a residual oil. This was purified by flash chromatography using a log ISCO™ column and 2.5% MeOH/CH₂Cl₂ to obtain the title compound (56 mg, 56% yield). Rf=0.30, (10% MeOH/CH₂Cl₂): LRMS m/z Calcd for C₁₈H₂₄ClFN₂O, 338.9, found, 339.4 (M+H), 319.4 (M+H−HF) APCI; ¹H-NMR (CDCl₃) δ 7.46 (d, J=7.9 Hz, 1H), 7.39 (bs, 1H), 7.28 (m, 1H), 3.72 (s, 2H), 3.68-3.57 (m, 1H), 2.97 (s, 3H), 2.95 (s, 3H), 2.96-2.66 (m, 4H), 2.60-2.52 (m, 4H), 1.82-1.74 (m. 4H); ¹³C-NNMR (CDCl₃) δ 173.3, 141.8 (dd. J_(C-F)=23.5 Hz), 137.0, 134.0, 130.7, 125.8 (d, J_(C-F)=8.3 Hz), 123.2 (d, J_(C-F)=8.0 Hz), 97.5 (d, J_(C-F)=194.0 Hz), 56.9, 54.4, 38.6, 38.4, 36.9, 35.8, 30.1, 23.8.

Where cis and trans isomers are possible for an embodiment of the inventive compound of formula I, both cis and trans isomers are within the scope of the invention. Rotomrers are possible for an embodiment of the inventive compound of formula I and are within the scope of the invention.

Conventional techniques for the preparationlisolation of individual enantiomners include chiral synthesis from a suitable optically pure precursor or resolution of the racernate (or the racemate of a salt or derivative) using, for example, chiral high pressure liquid chromatography (HPLC).

Alternatively, the racemate (or a racemic. precursor) may be reacted with a suitable optically active compound, for example, an alcohol, or, in the case where the compound of formula I contains an acidic or bazsic moiety, an acid or base such as tartaric acid or 1-phenylethyamine. The resulting diastereomeric mixture may be separated by chromatography andlor ftractional crystallization and one or both of the diastereoisomers converted to the corresponding pure enantiomer(s) by mreans well known to a skilled person.

Chiral compounds of the invention tand chiral precursors thereof) may be obtained in enantiomericallyenrinched form using chromatography, typically HPLC, on an asymmetrno resin wfth a tnobile phase consisting of a hydrocarbon, typically heptane or heaxane, containing from 0 to 50% by 'volume of isopropanol, typically from 2% to 20%, and from 0 to 5% by volume of an alkylamine, typically 0.1% diethylamine. Concentration of the eluate affords the enriched mixture.

Stereolsomeric conglomerates may be separated by conventional techniques known to those skilled in the art—see, for example, “Stereochemistr of Organic Compounds∞ by E. L. Eliel (Wiley, New York, 1994).

The composition of the present invention may be formulated in a conventional manner using one or more pharmaceutically acceptable carriers. The composition may be formulated for oral, buccal, intranasal. parenteral (e.g., intravenous, intramuscular. intraperitoneal, or subcutaneous or through an implant) nasal, vaginal, sublingual. rectal or topical administration or in a form suitable for administration by inhalation or insufflation.

Pharmaceutically acceptable salts of compounds of formula I may be prepared by one or more of three mnethods: (i) by reacting the corpound of formula I with the desired acid or base; (ii) by removing an acid or base-labie protecting group from a suitable precursor of the compound of formula I or by ring-opening a suitable cyclic precursor, for example, a lactone or lactam, using the desired acid or base; or (iii) by converting one salt of the compound of formula I to another by reaction with an appropriate acid or base or by means of a suitable ion exchange column.

All three reactions are typically carried out in solution. The resulting salt may precipitate out and be collected by filtration or may be recovered by evaporation of the solvent, The degree of ionisation in the resulting salt may vary from completely ionised to almost non-ionised.

Also included within the scope of the invention are metabolrtes of compounds of formula I, that is, compounds formed in vivo upon administration of the drug. Some examples of mnetaboiftes in accordance Mth the invention include: (i) where the coinpound of formula (I) contains a methyl group, an hydroxymethyl derivative thereof (—CH₃→—CH₂OH); (ii) where the compound of formula (I) contains an alkoxy group, an hydroxy derivative thereof (—OR→—OH); (iii) wthere the compound of formula (I) contains A tertiary amino group, A secondary amino derivative thereof (—NR^(a)R^(b)→—NHR¹ or —NHR^(b)); (iv) where the cormpouncd of formula (I) contains a secondary amino group, a primary derivative thereof (—NHR^(a)→—NH₂); (v) where the compound of formula (I) contains an amide group, a carboxyli3c acid derivative thereof (—CONR^(c)R^(d)→COOH).

Isotopically labeled compounds of formula I of this invention can generally be prepared by carrying out the procedures disclosed in the preceeding Schemes andior in the Examples and Preparations, by substituting a readily available isotopically labeled reagent for a non-isotopically labeled reagent.

For oral administration, the pharmaceutical composition may take the form ot, for example, tablets or capsules prepared by conventional mneans with pharmaceutically acceptable excipients such as binding agents such as pregelatinized maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose; fillers such as lactose, microcrystalline cellulose or calcium phosphate: lubricants such as magnesium stearate, talc or silica: disintegrants such as potato starch or sodium starch glycolate; or wetting agents such as sodium lauiryl sulphate. The tablets may be coated by methods weil knowvn in the art. Liquid preparations for oral administration may take the form of, for example, solutions, syrups or suspensions, or they may be presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations may be prepared by conventional means with pharrmaceutically acceptable additives such azs suspending agents such as sorbitol syrup, methyl cellulose or hydrogenated edible fats, emulsifying agents such as lecithin or acacia, non-aqueous vehicles such as almond oil, oily esters or ethyl alcohotl and preservatives such as methyl or propyl p-hydroxybenzoates or sorboc acid.

For buccal administration, the composition may take the form of tablets or lozenges formulated in conventional manner.

The composition of the invention mnay be fornulated for parenteral admininstration by injection, including using conventional catheterization techniques or infusion. Formulations for injection may be presented in unit dosage form, for example, in ampoules or in multi-dose containers, with an added preservative. The composition may take such forns as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formuilating agents such as suspending, stabilizing andfor dispersing agents. Alternatively, the active ingredient or ingredients in a composition may be in powder form for reconstitution with a suitable vehicle, for exaymple sterie pyrogen-free water, before use. The termu “active ingredient” as used herein refers to a compound of the formula I, a histamiine H₁ antagonist, or a neurotransmnitter re-upptake blocker.

The composition of the invention may also be fornulated in a rectal composition such as suppositories or retention enemas, for example, containing conventional suppository bases such as cocoa butter or other glycerides. A composition for vaginal administration is preferably a suppository that may contain: in addition to the active ingredient or ingredients, excipients such as cocoa butter or a suppository wax. A composition for nasal or sublingual administration is also prepared with standard excipients wVell known in the art.

For intranasal administration or administration by inhalation, the composition may be conveniently delivered in the form of a solution or suspension from a pu-mp spray container that is squeezed or pumped by the patient or as an aerosol spray presentation from a pressurized container or a nebulizer, with the use of a suitable propellant, for example, dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol, the dosage unit tmay be determined by providing a valve to deliver a metered amount. The pressurized container or nebulizer may contain a solution or suspension of the active ingredient or ingredients. Capsules and cartridges, made, for example, from gelatin, for use in an inhaler or insufflator may be fornmulated containing a powder mix of an active ingredient or ingredients and a suitable powder base such as lactose or starch The active ingredient or ingredients in the composition may range in size from nanopalicles to micropalicles.

An exemplary dose of the composition of the invention comprising a compound of formula I for oral, parenteral or buccal administration to the average adult human for the treatment of the conditions referred to herein is about 0.01 to about 1000 mg of the compound of formula I per unit dose which could be administered, for example, 1 to 3 times per day.

An exemplary dose of the composition of the invention comprising a coompound of formula I and a histamine H₁ antagonist or a neurotransmitter re-uptakle blocker for oral, parenterai or buccal administration to the average adult human for the treatment of the conditions referred to herein is about 0.01 to about 500 mg of the compound of forinula I and of about 0.01 mng to about 500 mng of the histamine H₁ antagonist or the neturotransmitter re-uptake blocker per unit dose wvhich could be Administered, for examnple, 1 to 3 times per day.

Aerosol formulations for treatment of the conditions referred to herein in the average adult human are preferably arranged so that each metered dose or “puff” of aerosol contains about 20 μg to about 1000 μg of the compound of formula I. The overall daily dose with an aerosol wfill be within the range about 100 μg to about 10 mg. Administration may be several times daily. for example 2, 3, 4 or 8 times, giving for example, 1, 2 or 3 doses each time. Aerosol formulations containing a compound of formula I and a histamine H₁ antagonist or a neurotransmitter re-uptake blocker are preferably arranged so that each metered dose or “puff” of aerosol contains about 100 μg to about 10,000 μg of the compound of formula I and about 100 μg to about 30,000 μg of the histamine H₁ antagonist or the neurotransnitter re-uptake blocker. Administration may be several times daily, for example 1, 3, 4 or 8 times, giving for example, 1, 2 or 3 doses each time. The composition of the invention comprising a compound of formula I and a histamine H₁ antagonist or a neurotransmitter re-uptake blocker may optionally contain a pharmaceutically acceptable carrier and may be administered in both single and multiple dosages as a variety of different dosage forms, such as tablets, capsules, lozenges, troches, hard candies, powders, sprays, aqueous suspension, injectable solutions, elixirs, syrups, and the like. The pharmaceutically acceptable carriers include solid diluents or fillers, sterile aqueous media and various non-toxic organic solvents, etc. Oral pharrnaceutical formtuiations can be suitably sweetened andfor flavored by means of various agents of the type commonly employed for such purposes. In general, the compound of formlula u s present in sUch dosage form-s ait concentration levels ranging from about 0.1% to about 99.9% by weight of the total composition, i.e., in amounts which are sufficient to provide the desired unit dosage, and the histamine H, antagonist or the neulrotransmitter re-uptake blooker is present in sucih dosage forms at concentration levels ranging from about 0.1% to about 99.9% by wevight of the total composition, i.e., in amounts which are sufficient to provide the desired unit dosage.

The compound of formula I and the histamine H₁ antagonist may be administered together or separately When administered separately, the compound of formula I and the histamine H₁ antagonist may be administered in either order, provided that after administration of the first of the tevo active ingredients, the second active ingredient is administered Witn 24 hours or less, preferably 12 hours or less.

The compound of formula I and the neurotransiritter reuptake blocker may be administered together or separately. When administered separately, the coompound of formrula I and the neulrotransmttter re-uptake blocker may be administered in either order, provided that after administration of the first of the tbo active ingredients, the second active ingredient is administered within 24 hours or less, preferably 12 hours or less.

A preferred dose ratio of compound of forrnula I to the histamine H₁ antagonist or to the neurotransmitter re-uptake blocker for oral, parenteral or buccal administration to the average adult human for the treatment of the conditions referred to herein is from about 0.001 to about 1000, preferably from about 0.01 to about 100.

The composition may be homogeneous, wherein by homogeneous it is meant that the active ingredient or ingredients are dispersed evenly throughout the composition so that the composition may be readily subdivided into equally effective unit dosage forms such as tablets, pills and capsules. This solid composition is then subdivided into unit dosage forms of the type described herein containing from about 0.1 to about 1000 mg of the active ingredient or ingredients. Typical unit dosage forms contain from about I to about 300 mg, for example about 1, 2, 5, 10, 25, 50 or 100 mg, of the active ingredient or ingredients. The tablets or pills of the novel composition can be coated or otherwtise compounded to provide a dosage form affording the advantage of prolonged action. For example, the tablet or pill can comprnse an inner dosage and an outer dosage cornponent, the latter being in the form of an envelope over the former, The two components can be separated by an enteric layer which senies to resist disintegration in the stomach and permits the inner component to pass intact into the duodenum or to be delayed in release. A variety of materials can be used for such enteric layers or coatings, such materials including a number of polymeric acirds and mixtures of polymeric acids with such materials as shellac, cetyl alcohol and cellulose acetate.

The dosage of the active ingredient or ingredients in the composition and methods of this invention may be varied; however, it is necessary that the amount of the active ingredient or ingredients in such a composition be such that a suitable dosage forrn is obtained. The selected dosage depends upon the desired therapeutic effect, on the route of administration. the particular compounds administered, the duration of the treatment, and other factors. All dosage ranges and dosage levels mentioned herein refer to each active ingredient present in the pharmaceutical composition of the present invention, as wvell as those used in the methods of the present invention. Generally, dosage levels of between about 0.01 and about 100 mg/kg of body weight daily are administered to humans and other mammals. A preferred dosage range in humans is about 0.1 to about 50 mg/kg of body weight daily which can be administered as a single dose or divided into multiple doses. A preferred dosage range in mammals other than humans is about 0.01 to about 10.0 mg/kg of body weight daily which can be administered as a single dose or divided into multiple doses, A more preferred dosage range in mammals other than humans is about 0.1 to about 5.0 mg/kg of body veight daily which can be administered as a single dose or divided into multiple doses.

The pharmaceutical composition comprising the compound of formufla I and the histamine H₁ antagonist or the neurotransmitter re-tptake blocker may be administered at dosages of a therapeutically effe ative amount of the compound of formula I and of the second active ingredient in single or divided doses.

The specific therapeutically effective dose level for any particular patient will depend upon a variety of factors including the disorder being treated and the severity of the disorder, activity of the specific compound employed; the specific composition employed; the age. However some variation in dosage will necessarily occur depending upon the condition of the subject being treated. The person responsible for administration will, in any event. determine the appropriate dose for the individual subject.

The dosage amounts set forth in this description and in the appended claims may be used, for example, for an average hurman subject having a weight of about 65 kg to about 70 kg. The skilled practitioner will readily be able to determine any variation in the dosage amnount that may be required for a subject whose weight falls outside the about 65 kg to about 70 kg range, based upon the medical history of the subject. The pharmaceutical combinations may be administered on a regimen of up to 6 times per day, preferably 1 to 3 times per day, such as 2 times per day or once daily.

Cermination of Biological Activity

The in vitro affinity of the compounds in the present invention at the rat or human histamine H3 receptors can be determined a-cording to the following procedure. Frozen rat frronta brain or frozen human post-mortem frontal brain is homogenized in 20 volumes of cold 50 mkM Tris HCl containing 2 mM MgCl₂ (pH to 7.4 at 4° C.). The homogenate is then centrifuged at 45,000 G for 10 minutes, The supernatant is decanted and the membrane pellet resuspended by Polytron in cold 50 mM Tris HCl containing 2 mM MgCl2 (pH to 7.4 at 4° C.) and centrifuged again. The final pellet is resuspended in 50 mM Tris HCl containing 2 mM MgCl2 (pH to 7.4 at 25° C.) at a concentration of 12 mg/mL. Dilutions of compounds are made in 10% DMSO/50 mM Tris buffer (pH 7.4) (at 10× final concentration, so that the final DMSO concentration is 1%). Incubations are initiated by the addition of membranes (200 microliters) to 96 well V-bottoim polypropylene plates containing 25 microliters of drug dilutions and 25 microliters of radioligand (1 nM final concentration 3H—N-methyl-histamine). After a 1 hour incuibation, assay samples are rapidly filtered through Whatnian GF/B filters and rinsed wAth ice cold 50 mM Tris buffer (pH 7.4) using a Skatron cell harvester, Radioactivity is quantified using a BetaPlate scintillation counter. The percent inhibition of specific binding can then be calculated.

A person of ordinary skiil in the art could adapt the above procedure to other assays. TABLE 1 Rat Histamine H3 Receptor Binding Example # rH3 K_(i) (nM) 9 18.9 11 24.1 13 10.1 15 44.1 16 20.7 23 28.8 46 10.9 50 32.3 55 10.1 59 21.4 

1. A compound of formula I

or a pharmaceutically acceptable salt thereof, wherein. Rhu 1 and R² are each independently selected from the group consisting of hydrogen, C₁-C₈ alkyl optiornally substituted with 1 to 4 hlalogens; C₁-C₄ alkyl group optionally substituted with a substituent selected from the group consisting of OH, one to fOLur C₁-C₄ alkyl, C₃-C₇ cycloalkyl C₁-C₄ dialkylatmino C₆-C₁₄ aryl optionally substitued with a halogen and optionally substituted with C₆-C₁₀ aryloxy optionally substituted with one to two halogens, and 5-10membered heteroaryl optionally substituted with a C₆-C₁₀ aryl group and optionally substituted with one to three C₁-C₄ alkyl groups; C₃-C₇ cycloalkyl; C₈-C₁₄ aryl; -(C₀-C₃)alkyl-O-(C₁-C₃)alkyl optionally substituted with (C₁-C₃)alkyl; -(C₁-C₃)alkyl-C(═O)O-(C₁-C₃)alkyl; 3-8-membered heterocycloalkyl optionally substituted with one or more C₁-C₄ alkyl-carbonyl groups; C⁸-C₁₀arylsulfonyl optionally substituted with one or more C₁-C₂ alkyl: 5-10-membered heteroaryl; and C₈-C₁₄ aryl-C₀-C₄ alkylene-O-C₀-C₄ alkyl, wherein each C₀-C₄ alkyl and each C₀-C₄ alkylene is optionally subtstituted with one to four (C₁-C₄ alkyl; or optionally R¹ and R², together with the nitrogen to which they are attached, form a 4-, 5-, 6-, or 7-membered saturated or unsaturated aliphatic ring wherein one of the carbons in said aliphatic ring is optionally replaced by O, S, NR³, or CO, and wherein said ring is optionally fused to a C₈-C₁₀ arylene and is optionally substituted at a ring carbon with a substituent selected from the group consisting of —OH, 5-10-membered heteroaryl optionally substituted with one or more halogens and optionally substituted kit}h one or more C₁-C₂ alkyl, C₁-C₄ alkoxy optionally substituted with one or more C₁-C₂ alkoxy and optionally substituted with one or more C₁-C₄ dialkylaminocarbonyl, and one or two C₄-C₄ alkyl optionally and independently substituted with one or more C₁-C₂ alkoxy; wherein R³ is hydrogen, C₁-C₈ alkyl optionally substituted with 1 to 4 halogens; 5-10-membered heteroaryl optionally substituted with a substituent selected from the group consisting of halogen, C₁-C₄ alkyl, C₁-C₂ alkoxy, C₆-C₁₀ aryl, C₁-C₄ alkylaminocarbonyl, and cyano; C₁-C₄ alkyl group optionally substituted with a substituent selected from the group consisting of C₁-C₂ alkoxycarbonyl, 5-10-membered heteroaryl optionally substituted with one or more C₁-C₂ alkyl, one to four C₁-C₄ alkyl, C₃-C₇ cycloalkyl, and C₈-C₁₄ aryl; C₈-C₁₀ aryl optionally substituted with one or C₁-C₂ alkyl; C₁-C₄ alkylcarbonyl; or C₆-C₁₄ aryl-C₀-C₄ alkylene-O-C₀-C₄ alkyl, wherein each C₀-C₄ alkyl and each C₀-C₄ alkylene is optionally substituted with one to four C₁-C₄ alkyl; R⁴ is independently selected from the group consisting of hydrogen, C₁-C₄ alkyl: C₁-C₄ alkoxyl, halogen, nitnie, —SO₂C₁-C₄, —SO₂NHC₁-C₄ and —C(═O)NHC₁-C₄; n is 0, 1, 2, 3, or 4; R⁵ is OH, —O(C₁-C₃)alkyl, halogen or hydrogen; R⁶ is hydrogen, C₁-C₄ alkyl optionally substituted with 1 to 4 halogens, or C₃-C₇ cycloalkyl-C₀-C₄ alkyl; R⁷ is hydrogen, C₁-C₆ alkyl optionally substituted with 1 to 4 halogens, or C₃-C₇ cycloalkyl-C₀-C₄ alkyl, wherein each C₀-C₄ is optionally substituted with one to four C₁-C₄ alkyl and; R⁸ is hydrogen: C₁-C₈ alkyl optionally substituted with 1 to 4 halogens, or C₃-C₇ cycloalkyl-C₀-C₄ alkyl; or optionally R⁷ and R⁸ together with the nitrogen to which they are attached, form a 4-, 5-, 6-, or 7-membered heterocyclic ring, wherein said heterocyclic ring is optionally substituted with one or two C₁-C₄ alkyl; and wherein one of the carbons of said heterocyclic ring that is separated by at least two atoms from said nitrogen in said heterocyclic ring is optionally replaced by O, S, NR⁹ or C═O wherein R⁹ is hydrogen, C₁-C₈ alkyl optionally substituted with 1 to 4 halogens, or C₃-C₇ cycloalkyl-C₀-C₄ alkyl, and wherein each C₀-C₄ alkyl is optionally substituted with one to four C₁-C₄ alkyl.
 2. A compound of formula I, according to claim 1, wherein R⁷ and R⁸, together with the nitrogen to which they are attached form a 4-, 5-, 6- or 7-membered heterocyclic ring, wherein said heterocyclic ring is optionally substituted with one or two C₁-C₄ alkyl; and wherein one of the carbons of said heterocyclic ring that is separated by at least two atoms from said nitrogen in said heterocyclic ring is optionally replaced by O, S, NR⁹, or C═O, wherein R⁹ is hydrogen, C₁ -C₈ alkyl optionally substituted with 1 to 4 halogens, or C₃-C₇ cycloalkyl-C₀-C₄ alkyl, and wherein each C₁-C₄ alkyl is optionally substituted with one to four C₁-C₄ alkyl.
 3. A compound of formula I, according to claim 2, wherein R⁷ and R⁸, together with the nitrogen to which they are attached, form a 5- or 6-membered saturated heterocycle.
 4. A compound of formula I, according to claim 3, wherein said saturated heterocycle is a pyrrolidinyl group.
 5. A compound of formula I, according to claim 2, wherein R¹ is hydrogen; R⁴ and R⁵ are independently hydrogen or F; R⁶ is hydrogen or C₁-C₆ alkyl.
 6. A compound of formula 1, According to claim 1, wherein R⁵ is H.
 7. A compound of formula 1, according to claim 1, wherein R⁵ is F.
 8. A compound of formula 1, according to claim 2, wherein R⁵ is H.
 9. A compound of formula 1, according to claim 2, wherein R⁵ is F.
 10. A cis cyclobutyl isomer of formula I, according to claim
 1. 11. A trans cyclobutyl isomer of formula I, according to claim
 1. 12. A cis cyclobuLtyl isomer of formula I, according to claim
 2. 13. A trans cyclobutyl isomer of formula I, according to claim
 2. 14. A compound of formula I, or a pharmaceutically acceptable salt thereof, according to claim 1, wherein the compound is selected from the group consisting of: cis-3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecaboxylic acid dimethylamide; cis-[3(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl-methanone; cis-3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethyl-methyl-amide; cis-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide; cis-3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide; cis-{3-[3-Chloro-4-((R)-2-methyl-pyrrolidin-1-ylmethyl)-phenyl]-cyclobutyl}-pyrrolidin-1-yl-methanone; cis-3-(2,3-Dichloro-4-pyrrolidin-1-ylnethyl-phenyl)-cyobutanecarboxylic acid dimethylamide; cis-3-(3-Fluoro-4-pyrroldin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethylamide; cis-3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic aced isobutyl-amide; cis-(3-Aza-bicyclo[3.2.2]non-3-yl)-[3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-methanone; trans-3-(3-Fluoro-4-pyrrolidin-ylmethyl-phenyl)acyclobutanecarboxylic acid dimethyliamide; trans-[3-(3-Fluoro-4-pyrroldin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl-methanone; trans-3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethyl-methyl-amide; trans-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide; trans-3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide; trans-{3-[3-Chloro-4-((R)-2-methyl-pyrrolidin-1-ylmethyl)-phenyl]-cyclobutyl}-pyrroiidin-1-yl-methanone; trans-3-(2,3-Dichloro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid dimethylamide; trans-3-( 3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanLecarboxylic acid ethylamide; trans-3-(3-Fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxyic acid isoblutyl-amide; and trans-(3-Aza-bicyclo[3.2.2]non-3-yl)-[3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-methanone.
 15. A compound of formula I, or a pharmaceutically acceptable salt thereof, according to claim 1, wherein the compound is selected from the group consisting of: cis-[3- Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cycobutyl]-pyrrolidin-1-yl-methanone; cis-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid dimethylarimde; cis-[3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutyl]-piperidin-1-yl-methanone; cis-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxyic acid isobutfyl-methyl-amide; cis-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid cyclopropylmethyl-amide; cis-[3-(3,5-Difluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutyl]-pyrrolidin-1-yl-methanone; cis-3(2,6-Difluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxyic acid methylamide; cis-3-(5-Chloro-2-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid isobutyl-amide; cis-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid ethylamide; cis-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid methyl-(tetrahydro-pyran-4-ylmethyl)-amide; cis-3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide; cis-{3-[3-Chloro-4-((R)-2-methyl-pyrrolidin-1-ylmnethyl)-phenyl]-3-fluoro-cyclobutyl}-pyrrolidin-1-yl-methanone; sis-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid cyclopropylmethyl-methyl-amide; cis-3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid dimethylamide; cis-3-Fluoro-3-[3-fluoro-4-((S)-2-methyl-pyrrolidin-1-ylmethyl)-phenyl]-cyclobutanecarboxylic, acid ethylamide; cis-[3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutyl]-(2,3-dihydro-5H-benzo[f][1,4]oxazepin-4-yl)-methanone; cis-3-Fluoro-3-(3-fluoro-4-pyrrlidin-1-ylmethyl-phenyl)-cyclobutanecarhoxylic acid ethylamide; cis-3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarxyic acid ethyl-methyl-amide; cis-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid methyl(3-methyl-pyridin-2-ylmethyl)-amide; cis-3-Fluoro-3-[3-fluoro-4-((R)-2-methyl-pyrrolidin-1-ylmethyl)-phenyl]-cyclobutanecarboxylic acid ethylamide; cis-3-Fluoro-3-(3-fluoro-4-pyroliddn-1-ylmethyl-phenyl)-cyclobutanecarboxyidc acid isobutyl-amide; cis-[3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl-cyclobutyl]-pyrrolidin-1-yl-methanone; cis-[3-Fluoro-3-(3-flucro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl-methanone; cis-3-(2,3-Dichloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarbaoxyiic acid dimethylamide; trans-[3-Fluoro-3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl-methanone; trans-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid dimethylamide; trans-[3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutyl]-piperidin-1-yl-methanone; trans-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid isobutyl-methyl-amide; trans-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid cyclopropylmethyl-amide; trans-[3-(3,5-Difluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutyl]-pyrroldin-1-yl-methanone; trans-3-(2,6-Difluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid methylanmide; trans-3-(5-Chloro-2-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid isobutyl-amide; trans-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid ethylamide; trans-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid methyl-(tetrahydro-pyran-4-ylmethyl)-amide; trans-3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid methylamide; trans-{3-[3-Chloro-4-((R)-2methyl-pyrrolidin-1-ylmethyl)-phenyl]-3-fluoro-cyclobutyl}-pyrrolidin-1-yl-methanone; trans-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid cyclopropylmethyl-methyl-amide; trans-3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ymethyl-phenyl)-cyclobutanecarkboxylic acid dimethylamide; trans-3-Fluoro-3-[3-fluoro-4-((S)-2-methyl-pyrrolidin-1-ylmethyl)-phienyl]-cycrlobutanecarboxylic acid ethylamide; trans-[3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutyl]-(2,3-dihydro-5H-benzo[f][1,4]oxazepin-4-yl)-methanone; trans-3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethylamide; trans-3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxylic acid ethyl-methyl-amide; trans-3-(3-Chloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboyidc acid methyl-(3-methyl-pyridin-2-ylmethyl)-amide; trans-3-Fluoro-3-[3-fluoro-4-((R)-2-methyl-pyrroldin-1-ylmethyl)-phenyl]-cyclobutanecarboxylic acid ethylamide; trans-3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutanecarboxyiic acid isobutyl-amide; trans-[3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl-methanone; trans-[3-Fluoro-3-(3-fluoro-4-pyrrolidin-1-ylmethyl-phenyl)-cyclobutyl]-pyrrolidin-1-yl-methanone; and trans-3-(2,3-Dichloro-4-pyrrolidin-1-ylmethyl-phenyl)-3-fluoro-cyclobutanecarboxylic acid dimethylamide.
 16. A pharmaceutical composition for treating a disorder or condition that may be treated by antagonizing histamine-3 receptors, the composition comprising a compound of formula I as described in claim 1, and optionally a pharmaceutically acceptable carrier.
 17. A method of treatment of a disorder or condition that may be treated by antagonizing histamine-3 receptors, the method comprising administering to a mammal in need of such treatment a compound of formula I, or a pharmaceutically acceptable salt thereof, as described in claim
 1. 18. The method of claim 17 selected from the group consisting of depression. mood disorders, schizophrenia, anxiety disorders, cognitive disorders, Alzheimer's disease, attention-deficit disorder, attention-deficit hyperactivity disorder, psychotic disorders, sleep disorders, obesity, dizziness, epilepsy, motion sickness, respiratory diseases, allergy, allergy induced airway responses, allergic rhinitis, nasal congestion, allergic congestion, congestion, hypotension, cardiovascular disease, diseases of the GI tract, hyper and hypo motility and acidic secretion of the gastro intestinal tract, the method comprising administering to a mammal in need of such treatment a compound of formula I as described in claim
 1. 19. The method of claim 18, wherein the disorder or condition is selected from the group consisting of anxiety disorders, attention-deficit hyperactivity disorder, attention-deficit disorder, respiratory diseases, obesity, cognitive disorders, and psychotic disorders.
 20. The method of claim 18, wherein the disorder or condition is a respiratory disease selected from the group consisting of adult respiratory distress syndrome, acute respiratory distress syndrome, bronchitis, chronic bronchitis, chronic obstructive pulmonary disease. cystic fibrosis. asthma, emphysema. rhinitis and chronic sinusitis.
 21. A pharmaceutical composition for treating allergic rhinitis, nasal congestion or allergic congestion comprising: (a) an H3 receptor antagonist compound of formula I; or a pharmaceutically acceptable salt thereof; (b) an H1 receptor antagonist or a pharmaceutically acceptable salt thereof; and (c) a pharmaceutically acceptable carrier, wherein the active ingredients (a) and (b) above are present in amounts that render the composition effective in treating allergy rhinitis, nasal congestion or allergic congestion.
 22. The pharmaceutical composition according to claim 21, wherein said H1 receptor antagonist is selected from the group consisting of cetirizine chlorpheniramine, loratidine, fexofenadine, and desloradine.
 23. A pharmaceutical composition for treating attention-deficit disorder, attention-deficit hyperactivity disorder, depression, mood disorders, or cognitive disorders comprising: an H3 receptor antagonist compound of Formula I or a pharmaceutically acceptable salt thereof; a neurotransmitter re-uptake blocker or a pharmaceutically acceptable salt thereof; a pharmaceutically acceptable carrier; wherein the active ingredients (a) and (b) above are present in amounts that render the composition effective in treating depression, mood disorders, and cognitive disorders.
 24. The pharmaceutical composition according to claim 23, wherein the neurotransmitter re-uptake blocker is selected from the group consisting of sertraline, fluoxetine and paroxetine.
 25. A process for the preparation of a compound according to formula I in claim 1, wherein the process comprises the step of reacting a compound of the formula 4,

with an organo metallic reagent derived from a compound of formula 2,

followed by the direct amide formation to yield a compound of the formula I. 